LOCUS       BBa_J24719              2764 bp    DNA     linear       24-Dec-2024
DEFINITION  39. pLacI=M RBS=AraC=S RBS=TetR=Strong RBS=mCherry(+LVA)=terminator
ACCESSION   BBa_J24719
VERSION     BBa_J24719.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..1103
                     /label=araC
     misc_feature    1104..1136
                     /label=LVA
     misc_feature    1143..1167
                     /label=Help:Barcodes
     CDS             228..1167
                     /label=BBa_C0080
     misc_feature    1..1167
                     /label=BBa_J24692
     misc_feature    1180..1183
                     /label=conserved
     RBS             1176..1187
                     /label=BBa_B0034
     CDS             1197..1813
                     /label=tetR
     misc_feature    1814..1847
                     /label=SsrA
     misc_feature    1854..1878
                     /label=Help:Barcodes
     CDS             1194..1878
                     /label=BBa_C0040
     misc_feature    1176..1878
                     /label=BBa_J24688
     misc_feature    1891..1894
                     /label=conserved
     RBS             1887..1898
                     /label=BBa_B0034
     CDS             1905..2612
                     /label=mCherry
     misc_feature    2256..2256
                     /label=C->T (removing PstI site)
     misc_feature    2613..2621
                     /label=LVA
     misc_feature    1905..2627
                     /label=BBa_J06505
     stem_loop       2647..2690
                     /label=stem_loop
     misc_feature    2636..2715
                     /label=BBa_B0010
     terminator      2636..2715
                     /label=BBa_B0010
     stem_loop       2731..2750
                     /label=T7 TE
     misc_feature    2757..2757
                     /label=stop
     polyA_site      2751..2764
                     /label=polya
     misc_feature    2724..2764
                     /label=BBa_B0012
     terminator      2724..2764
                     /label=BBa_B0012
     misc_feature    1887..2764
                     /label=BBa_J24702
     misc_feature    1176..2764
                     /label=BBa_J24707
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc
      241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc
      301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
      361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
      421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc
      481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc
      541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag
      601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag
      661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg
      721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt
      781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt
      841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
      901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc
      961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat
     1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
     1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1141 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatgtcca
     1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
     1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg
     1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg
     2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2761 tata
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