LOCUS       BBa_J24720              2765 bp    DNA     linear       02-May-2025
DEFINITION  40. 15(BBa_J24693)==25(BBa_J24705)
ACCESSION   BBa_J24720
VERSION     BBa_J24720.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1102
                     /label=araC
     misc_feature    1103..1135
                     /label=LVA
     misc_feature    1142..1166
                     /label=Help:Barcodes
     CDS             227..1166
                     /label=BBa_C0080
     misc_feature    1..1166
                     /label=BBa_J24693
     misc_feature    1181..1184
                     /label=conserved
     RBS             1175..1188
                     /label=BBa_B0031
     CDS             1198..1814
                     /label=tetR
     misc_feature    1815..1848
                     /label=SsrA
     misc_feature    1855..1879
                     /label=Help:Barcodes
     CDS             1195..1879
                     /label=BBa_C0040
     misc_feature    1175..1879
                     /label=BBa_J24686
     misc_feature    1892..1895
                     /label=conserved
     RBS             1888..1899
                     /label=BBa_B0034
     CDS             1906..2613
                     /label=mCherry
     misc_feature    2257..2257
                     /label=C->T (removing PstI site)
     misc_feature    2614..2622
                     /label=LVA
     misc_feature    1906..2628
                     /label=BBa_J06505
     stem_loop       2648..2691
                     /label=stem_loop
     misc_feature    2637..2716
                     /label=BBa_B0010
     terminator      2637..2716
                     /label=BBa_B0010
     stem_loop       2732..2751
                     /label=T7 TE
     misc_feature    2758..2758
                     /label=stop
     polyA_site      2752..2765
                     /label=polya
     misc_feature    2725..2765
                     /label=BBa_B0012
     terminator      2725..2765
                     /label=BBa_B0012
     misc_feature    1888..2765
                     /label=BBa_J24702
     misc_feature    1175..2765
                     /label=BBa_J24705
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca
      241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc
      301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
      361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
      421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca
      481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca
      541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc
      601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg
      661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga
      721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta
      781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg
      841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag
      901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc
      961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt
     1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa
     1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata
     1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaaccta ctagatgtcc
     1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
     1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc
     1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac
     2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag
     2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2761 ttata
//