LOCUS       BBa_J24722              2763 bp    DNA     linear       02-May-2024
DEFINITION  42. 15(BBa_J24693)==27(BBa_J24707)
ACCESSION   BBa_J24722
VERSION     BBa_J24722.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1102
                     /label=araC
     misc_feature    1103..1135
                     /label=LVA
     misc_feature    1142..1166
                     /label=Help:Barcodes
     CDS             227..1166
                     /label=BBa_C0080
     misc_feature    1..1166
                     /label=BBa_J24693
     misc_feature    1179..1182
                     /label=conserved
     RBS             1175..1186
                     /label=BBa_B0034
     CDS             1196..1812
                     /label=tetR
     misc_feature    1813..1846
                     /label=SsrA
     misc_feature    1853..1877
                     /label=Help:Barcodes
     CDS             1193..1877
                     /label=BBa_C0040
     misc_feature    1175..1877
                     /label=BBa_J24688
     misc_feature    1890..1893
                     /label=conserved
     RBS             1886..1897
                     /label=BBa_B0034
     CDS             1904..2611
                     /label=mCherry
     misc_feature    2255..2255
                     /label=C->T (removing PstI site)
     misc_feature    2612..2620
                     /label=LVA
     misc_feature    1904..2626
                     /label=BBa_J06505
     stem_loop       2646..2689
                     /label=stem_loop
     misc_feature    2635..2714
                     /label=BBa_B0010
     terminator      2635..2714
                     /label=BBa_B0010
     stem_loop       2730..2749
                     /label=T7 TE
     misc_feature    2756..2756
                     /label=stop
     polyA_site      2750..2763
                     /label=polya
     misc_feature    2723..2763
                     /label=BBa_B0012
     terminator      2723..2763
                     /label=BBa_B0012
     misc_feature    1886..2763
                     /label=BBa_J24702
     misc_feature    1175..2763
                     /label=BBa_J24707
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca
      241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc
      301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
      361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
      421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca
      481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca
      541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc
      601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg
      661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga
      721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta
      781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg
      841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag
      901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc
      961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt
     1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa
     1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata
     1141 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatgtccag
     1201 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga
     1261 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg
     1321 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca
     1381 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc
     1441 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac
     1501 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca
     1561 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg
     1621 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac
     1681 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga
     1741 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg
     1801 tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata
     1861 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga
     1921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     1981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2221 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa
     2281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     2401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     2461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     2521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     2581 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc
     2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2701 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2761 ata
//