##gff-version 3
##sequence-region BBa_J24724 1 3127
BBa_J24724	.	engineered_region	1	1022	.	+	0	ID=BBa_J24694;Name=BBa_J24694
BBa_J24724	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24694
BBa_J24724	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24724	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24724	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24724	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24724	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24724	.	CDS	83	1022	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24694
BBa_J24724	.	CDS	83	958	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24724	.	tag	959	991	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24724	.	engineered_tag	998	1022	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24724	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24694
BBa_J24724	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24724	.	engineered_region	1031	3127	.	+	0	ID=BBa_J24712;Name=BBa_J24712
BBa_J24724	.	engineered_region	2211	3127	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24712
BBa_J24724	.	terminator	2999	3078	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24724	.	stem_loop	3010	3053	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24724	.	engineered_region	2999	3078	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24724	.	terminator	3087	3127	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24724	.	stem_loop	3094	3113	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24724	.	polyA_site	3114	3127	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24724	.	stop_codon	3120	3120	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24724	.	engineered_region	3087	3127	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24724	.	ribosome_entry_site	2211	2222	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24724	.	conserved_region	2215	2218	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24724	.	engineered_region	2229	2990	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24724	.	sequence_alteration	2470	2470	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24724	.	engineered_region	2229	2990	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24724	.	stop_codon	2985	2990	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24724	.	tag	2946	2984	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24724	.	CDS	2229	2990	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24724	.	engineered_region	1031	2202	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24712
BBa_J24724	.	ribosome_entry_site	1031	1043	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24724	.	ribosome_entry_site	1031	1043	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24724	.	ribosome_entry_site	1037	1040	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24724	.	engineered_region	1031	1043	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24724	.	CDS	1050	2177	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24724	.	engineered_tag	2178	2202	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24724	.	engineered_region	1050	2177	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24724	.	tag	2139	2177	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24724	.	CDS	1050	2177	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_J24724
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctac
tagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccga
ttgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcg
cggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagag
attcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggc
atgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcag
cgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgag
caattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtc
agtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcg
tctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaag
ctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgc
gagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgt
caagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctct
tatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatgg
cggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctc
cagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtg
gtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggc
tgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttct
tgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctg
gtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggct
ggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttca
acaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgca
atgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagct
catgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgca
actctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaa
agaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggc
gacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttca
tctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccg
ctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttc
ttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctga
agggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatat
catggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctc
gccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagt
ccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactct
cggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata