##gff-version 3
##sequence-region BBa_J24725 1 3126
BBa_J24725	.	engineered_region	1031	3126	.	+	0	ID=BBa_J24713;Name=BBa_J24713
BBa_J24725	.	engineered_region	1031	2201	.	+	0	ID=BBa_J24685;Name=BBa_J24685;Parent=BBa_J24713
BBa_J24725	.	ribosome_entry_site	1031	1042	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24725	.	conserved_region	1035	1038	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24725	.	CDS	1049	2176	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24725	.	engineered_region	1049	2176	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24725	.	engineered_tag	2177	2201	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24725	.	CDS	1049	2176	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24725	.	tag	2138	2176	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24725	.	engineered_region	2210	3126	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24713
BBa_J24725	.	terminator	2998	3077	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24725	.	stem_loop	3009	3052	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24725	.	engineered_region	2998	3077	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24725	.	ribosome_entry_site	2210	2221	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24725	.	conserved_region	2214	2217	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24725	.	terminator	3086	3126	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24725	.	polyA_site	3113	3126	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24725	.	stop_codon	3119	3119	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24725	.	stem_loop	3093	3112	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24725	.	engineered_region	3086	3126	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24725	.	engineered_region	2228	2989	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24725	.	engineered_region	2228	2989	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24725	.	CDS	2228	2989	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24725	.	stop_codon	2984	2989	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24725	.	tag	2945	2983	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24725	.	sequence_alteration	2469	2469	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24725	.	engineered_region	1	1022	.	+	0	ID=BBa_J24694;Name=BBa_J24694
BBa_J24725	.	CDS	83	1022	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24694
BBa_J24725	.	CDS	83	958	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24725	.	engineered_tag	998	1022	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24725	.	tag	959	991	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24725	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24694
BBa_J24725	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24725	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24725	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24725	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24725	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24725	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24694
BBa_J24725	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_J24725
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctac
tagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccga
ttgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcg
cggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagag
attcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggc
atgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcag
cgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgag
caattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtc
agtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcg
tctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaag
ctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgc
gagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgt
caagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactacta
gagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctt
atcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggc
ggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcc
agtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtgg
tgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggct
gatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttctt
gatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctgg
tcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctg
gcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaa
caaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaa
tgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctc
atgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaa
ctctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaa
gaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcg
acgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcat
ctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgc
taccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttct
tcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaa
gggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatc
atggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcg
ccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtc
cgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctc
ggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata