LOCUS BBa_J24727 3126 bp DNA linear 26-Nov-2024 DEFINITION 47. 17(BBa_J24695)==32(BBa_J24712) ACCESSION BBa_J24727 VERSION BBa_J24727.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..957 /label=araC misc_feature 958..990 /label=LVA misc_feature 997..1021 /label=Help:Barcodes CDS 82..1021 /label=BBa_C0080 misc_feature 1..1021 /label=BBa_J24695 RBS 1030..1042 /label=RBS-3\Weak RBS 1036..1039 /label=RBS misc_feature 1030..1042 /label=BBa_B0032 RBS 1030..1042 /label=BBa_B0032 misc_feature 2138..2176 /label=LVA CDS 1049..2176 /label=lacI-LVA misc_feature 1049..2176 /label=BBa_C0012 misc_feature 2177..2201 /label=Help:Barcodes CDS 1049..2176 /label=BBa_C0012 misc_feature 1030..2201 /label=BBa_J24684 misc_feature 2214..2217 /label=conserved RBS 2210..2221 /label=BBa_B0034 CDS 2228..2989 /label=YFP (LVA) misc_feature 2984..2989 /label=2 misc_feature 2945..2983 /label=SsrA misc_feature 2469..2469 /label=A (Q->L) misc_feature 2228..2989 /label=BBa_E0032 misc_feature 2228..2989 /label=BBa_E0032 stem_loop 3009..3052 /label=stem_loop misc_feature 2998..3077 /label=BBa_B0010 terminator 2998..3077 /label=BBa_B0010 stem_loop 3093..3112 /label=T7 TE misc_feature 3119..3119 /label=stop polyA_site 3113..3126 /label=polya misc_feature 3086..3126 /label=BBa_B0012 terminator 3086..3126 /label=BBa_B0012 misc_feature 2210..3126 /label=BBa_J24704 misc_feature 1030..3126 /label=BBa_J24712 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac 121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat 181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt 241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg 301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac 361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata 421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg 481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata 541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca 601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc 661 aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat 721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc 781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt 841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg 901 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct 961 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1021 ctactagagt cacacaggaa agtactagat ggtgaatgtg aaaccagtaa cgttatacga 1081 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag 1141 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat 1201 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac 1261 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga 1321 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa 1381 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct 1441 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct 1501 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg 1561 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc 1621 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa 1681 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca 1741 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca 1801 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat 1861 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 1921 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 1981 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac 2041 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2101 gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc 2161 tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa 2221 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2281 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2341 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2401 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca 2461 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 2521 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2581 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 2641 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 2701 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 2761 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 2821 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 2881 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 2941 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca 3001 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3061 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3121 tttata //