LOCUS BBa_J24728 3125 bp DNA linear 27-Dec-2024 DEFINITION 48. 17(BBa_J24695)==33(BBa_J24713) ACCESSION BBa_J24728 VERSION BBa_J24728.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..957 /label=araC misc_feature 958..990 /label=LVA misc_feature 997..1021 /label=Help:Barcodes CDS 82..1021 /label=BBa_C0080 misc_feature 1..1021 /label=BBa_J24695 misc_feature 1034..1037 /label=conserved RBS 1030..1041 /label=BBa_B0034 misc_feature 2137..2175 /label=LVA CDS 1048..2175 /label=lacI-LVA misc_feature 1048..2175 /label=BBa_C0012 misc_feature 2176..2200 /label=Help:Barcodes CDS 1048..2175 /label=BBa_C0012 misc_feature 1030..2200 /label=BBa_J24685 misc_feature 2213..2216 /label=conserved RBS 2209..2220 /label=BBa_B0034 CDS 2227..2988 /label=YFP (LVA) misc_feature 2983..2988 /label=2 misc_feature 2944..2982 /label=SsrA misc_feature 2468..2468 /label=A (Q->L) misc_feature 2227..2988 /label=BBa_E0032 misc_feature 2227..2988 /label=BBa_E0032 stem_loop 3008..3051 /label=stem_loop misc_feature 2997..3076 /label=BBa_B0010 terminator 2997..3076 /label=BBa_B0010 stem_loop 3092..3111 /label=T7 TE misc_feature 3118..3118 /label=stop polyA_site 3112..3125 /label=polya misc_feature 3085..3125 /label=BBa_B0012 terminator 3085..3125 /label=BBa_B0012 misc_feature 2209..3125 /label=BBa_J24704 misc_feature 1030..3125 /label=BBa_J24713 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac 121 tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat 181 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt 241 cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg 301 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac 361 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata 421 tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg 481 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata 541 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca 601 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc 661 aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat 721 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc 781 caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt 841 ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg 901 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct 961 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1021 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat 1081 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 1141 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 1201 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 1261 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 1321 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 1381 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 1441 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 1501 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 1561 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 1621 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 1681 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 1741 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 1801 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 1861 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 1921 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 1981 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 2041 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2101 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 2161 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa 2221 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2401 cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac 2461 atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2821 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 2941 aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 3001 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3061 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3121 ttata //