##gff-version 3
##sequence-region BBa_J24728 1 3125
BBa_J24728	.	engineered_region	1	1021	.	+	0	ID=BBa_J24695;Name=BBa_J24695
BBa_J24728	.	CDS	82	1021	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24695
BBa_J24728	.	CDS	82	957	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24728	.	engineered_tag	997	1021	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24728	.	tag	958	990	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24728	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24695
BBa_J24728	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24728	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24728	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24728	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24695
BBa_J24728	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24728	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24728	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24728	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24728	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24728	.	engineered_region	1030	3125	.	+	0	ID=BBa_J24713;Name=BBa_J24713
BBa_J24728	.	engineered_region	1030	2200	.	+	0	ID=BBa_J24685;Name=BBa_J24685;Parent=BBa_J24713
BBa_J24728	.	CDS	1048	2175	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24728	.	engineered_region	1048	2175	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24728	.	engineered_tag	2176	2200	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24728	.	tag	2137	2175	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24728	.	CDS	1048	2175	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24728	.	ribosome_entry_site	1030	1041	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24728	.	conserved_region	1034	1037	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24728	.	engineered_region	2209	3125	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24713
BBa_J24728	.	terminator	2997	3076	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24728	.	stem_loop	3008	3051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24728	.	engineered_region	2997	3076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24728	.	ribosome_entry_site	2209	2220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24728	.	conserved_region	2213	2216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24728	.	terminator	3085	3125	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24728	.	polyA_site	3112	3125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24728	.	engineered_region	3085	3125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24728	.	stem_loop	3092	3111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24728	.	stop_codon	3118	3118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24728	.	engineered_region	2227	2988	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24728	.	sequence_alteration	2468	2468	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24728	.	stop_codon	2983	2988	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24728	.	CDS	2227	2988	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24728	.	engineered_region	2227	2988	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24728	.	tag	2944	2982	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
>BBa_J24728
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctta
tcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcg
gagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctcca
gtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggt
gtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctg
atcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttg
atgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggt
cgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctgg
cataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaac
aaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaat
gcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctca
tgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaac
tctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaa
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
caggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaag
aggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcga
cgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatc
tgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgct
accccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttctt
caaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaag
ggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatca
tggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgc
cgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtcc
gccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcg
gcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata