LOCUS BBa_J24729 3126 bp DNA linear 29-Apr-2024 DEFINITION 49. 18(BBa_J24696)==31(BBa_J24711) ACCESSION BBa_J24729 VERSION BBa_J24729.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..956 /label=araC misc_feature 957..989 /label=LVA misc_feature 996..1020 /label=Help:Barcodes CDS 81..1020 /label=BBa_C0080 misc_feature 63..1020 /label=BBa_J24682 misc_feature 1..1020 /label=BBa_J24696 misc_feature 1035..1038 /label=conserved RBS 1029..1042 /label=BBa_B0031 misc_feature 2138..2176 /label=LVA CDS 1049..2176 /label=lacI-LVA misc_feature 1049..2176 /label=BBa_C0012 misc_feature 2177..2201 /label=Help:Barcodes CDS 1049..2176 /label=BBa_C0012 misc_feature 1029..2201 /label=BBa_J24683 misc_feature 2214..2217 /label=conserved RBS 2210..2221 /label=BBa_B0034 CDS 2228..2989 /label=YFP (LVA) misc_feature 2984..2989 /label=2 misc_feature 2945..2983 /label=SsrA misc_feature 2469..2469 /label=A (Q->L) misc_feature 2228..2989 /label=BBa_E0032 misc_feature 2228..2989 /label=BBa_E0032 stem_loop 3009..3052 /label=stem_loop misc_feature 2998..3077 /label=BBa_B0010 terminator 2998..3077 /label=BBa_B0010 stem_loop 3093..3112 /label=T7 TE misc_feature 3119..3119 /label=stop polyA_site 3113..3126 /label=polya misc_feature 3086..3126 /label=BBa_B0012 terminator 3086..3126 /label=BBa_B0012 misc_feature 2210..3126 /label=BBa_J24704 misc_feature 1029..3126 /label=BBa_J24711 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact 121 cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt 181 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc 241 agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt 301 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc 361 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat 421 ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt 481 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa 541 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac 601 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca 661 attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc 721 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc 781 aggcgaagct gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg 841 gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga 901 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcagctg 961 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1021 tactagagtc acacaggaaa cctactagat ggtgaatgtg aaaccagtaa cgttatacga 1081 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag 1141 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat 1201 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac 1261 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga 1321 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa 1381 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct 1441 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct 1501 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg 1561 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc 1621 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa 1681 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca 1741 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca 1801 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat 1861 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 1921 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 1981 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac 2041 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2101 gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc 2161 tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa 2221 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2281 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2341 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2401 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca 2461 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 2521 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2581 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 2641 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 2701 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 2761 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 2821 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 2881 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 2941 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca 3001 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3061 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3121 tttata //