LOCUS BBa_J24730 3125 bp DNA linear 01-May-2025 DEFINITION 50. 18(BBa_J24696)==32(BBa_J24712) ACCESSION BBa_J24730 VERSION BBa_J24730.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..956 /label=araC misc_feature 957..989 /label=LVA misc_feature 996..1020 /label=Help:Barcodes CDS 81..1020 /label=BBa_C0080 misc_feature 63..1020 /label=BBa_J24682 misc_feature 1..1020 /label=BBa_J24696 RBS 1029..1041 /label=RBS-3\Weak RBS 1035..1038 /label=RBS misc_feature 1029..1041 /label=BBa_B0032 RBS 1029..1041 /label=BBa_B0032 misc_feature 2137..2175 /label=LVA CDS 1048..2175 /label=lacI-LVA misc_feature 1048..2175 /label=BBa_C0012 misc_feature 2176..2200 /label=Help:Barcodes CDS 1048..2175 /label=BBa_C0012 misc_feature 1029..2200 /label=BBa_J24684 misc_feature 2213..2216 /label=conserved RBS 2209..2220 /label=BBa_B0034 CDS 2227..2988 /label=YFP (LVA) misc_feature 2983..2988 /label=2 misc_feature 2944..2982 /label=SsrA misc_feature 2468..2468 /label=A (Q->L) misc_feature 2227..2988 /label=BBa_E0032 misc_feature 2227..2988 /label=BBa_E0032 stem_loop 3008..3051 /label=stem_loop misc_feature 2997..3076 /label=BBa_B0010 terminator 2997..3076 /label=BBa_B0010 stem_loop 3092..3111 /label=T7 TE misc_feature 3118..3118 /label=stop polyA_site 3112..3125 /label=polya misc_feature 3085..3125 /label=BBa_B0012 terminator 3085..3125 /label=BBa_B0012 misc_feature 2209..3125 /label=BBa_J24704 misc_feature 1029..3125 /label=BBa_J24712 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact 121 cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt 181 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc 241 agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt 301 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc 361 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat 421 ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt 481 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa 541 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac 601 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca 661 attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc 721 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc 781 aggcgaagct gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg 841 gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga 901 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcagctg 961 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1021 tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac gttatacgat 1081 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 1141 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 1201 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 1261 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 1321 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 1381 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 1441 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 1501 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 1561 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 1621 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 1681 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 1741 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 1801 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 1861 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 1921 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 1981 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 2041 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2101 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 2161 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa 2221 tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2401 cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta ccccgaccac 2461 atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2821 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 2941 aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 3001 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3061 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3121 ttata //