LOCUS BBa_J24731 3124 bp DNA linear 26-Nov-2024 DEFINITION 51. 18(BBa_J24696)==33(BBa_J247130) ACCESSION BBa_J24731 VERSION BBa_J24731.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..956 /label=araC misc_feature 957..989 /label=LVA misc_feature 996..1020 /label=Help:Barcodes CDS 81..1020 /label=BBa_C0080 misc_feature 63..1020 /label=BBa_J24682 misc_feature 1..1020 /label=BBa_J24696 misc_feature 1033..1036 /label=conserved RBS 1029..1040 /label=BBa_B0034 misc_feature 2136..2174 /label=LVA CDS 1047..2174 /label=lacI-LVA misc_feature 1047..2174 /label=BBa_C0012 misc_feature 2175..2199 /label=Help:Barcodes CDS 1047..2174 /label=BBa_C0012 misc_feature 1029..2199 /label=BBa_J24685 misc_feature 2212..2215 /label=conserved RBS 2208..2219 /label=BBa_B0034 CDS 2226..2987 /label=YFP (LVA) misc_feature 2982..2987 /label=2 misc_feature 2943..2981 /label=SsrA misc_feature 2467..2467 /label=A (Q->L) misc_feature 2226..2987 /label=BBa_E0032 misc_feature 2226..2987 /label=BBa_E0032 stem_loop 3007..3050 /label=stem_loop misc_feature 2996..3075 /label=BBa_B0010 terminator 2996..3075 /label=BBa_B0010 stem_loop 3091..3110 /label=T7 TE misc_feature 3117..3117 /label=stop polyA_site 3111..3124 /label=polya misc_feature 3084..3124 /label=BBa_B0012 terminator 3084..3124 /label=BBa_B0012 misc_feature 2208..3124 /label=BBa_J24704 misc_feature 1029..3124 /label=BBa_J24713 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact 121 cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt 181 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc 241 agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt 301 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc 361 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat 421 ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt 481 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa 541 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac 601 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca 661 attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc 721 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc 781 aggcgaagct gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg 841 gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga 901 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcagctg 961 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1021 tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg 1081 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 1141 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 1201 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 1261 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 1321 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 1381 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 1441 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 1501 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 1561 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 1621 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 1681 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 1741 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 1801 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 1861 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 1921 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 1981 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 2041 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 2101 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt 2161 tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat 2221 actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 2281 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2341 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2401 ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca 2461 tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2521 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2581 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2641 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 2701 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 2761 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 2821 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 2881 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 2941 agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg 3001 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 3061 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3121 tata //