LOCUS BBa_J24734 4027 bp DNA linear 27-Dec-2024 DEFINITION 54. 19(BBa_J24697)==30(BBa_J24710) ACCESSION BBa_J24734 VERSION BBa_J24734.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1225..1228 /label=conserved RBS 1219..1232 /label=BBa_B0031 misc_feature 2328..2366 /label=LVA CDS 1239..2366 /label=lacI-LVA misc_feature 1239..2366 /label=BBa_C0012 misc_feature 2367..2391 /label=Help:Barcodes CDS 1239..2366 /label=BBa_C0012 misc_feature 1219..2391 /label=BBa_J24683 misc_feature 1..2391 /label=BBa_J24697 misc_feature 2404..2407 /label=conserved RBS 2400..2411 /label=BBa_B0034 CDS 2421..3037 /label=tetR misc_feature 3038..3071 /label=SsrA misc_feature 3078..3102 /label=Help:Barcodes CDS 2418..3102 /label=BBa_C0040 misc_feature 2400..3102 /label=BBa_J24688 misc_feature 3115..3118 /label=conserved RBS 3111..3122 /label=BBa_B0034 CDS 3129..3890 /label=CFP (LVA) misc_feature 3847..3884 /label=SsrA misc_feature 3885..3890 /label=2 misc_feature 3197..3197 /label=A misc_feature 3129..3890 /label=BBa_E0022 misc_feature 3129..3890 /label=BBa_E0022 stem_loop 3910..3953 /label=stem_loop misc_feature 3899..3978 /label=BBa_B0010 terminator 3899..3978 /label=BBa_B0010 stem_loop 3994..4013 /label=T7 TE misc_feature 4020..4020 /label=stop polyA_site 4014..4027 /label=polya misc_feature 3987..4027 /label=BBa_B0012 terminator 3987..4027 /label=BBa_B0012 misc_feature 3111..4027 /label=BBa_J24703 misc_feature 2400..4027 /label=BBa_J24710 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa cctactagat ggtgaatgtg aaaccagtaa 1261 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 1321 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 1381 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 1441 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 1501 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 1561 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 1621 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 1681 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 1741 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 1801 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 1861 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 1921 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 1981 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 2041 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 2101 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 2161 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 2221 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2281 cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg 2341 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 2401 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 2461 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 2521 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 2581 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 2641 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 2701 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 2761 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 2821 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 2881 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 2941 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 3001 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 3061 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga 3121 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 3181 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 3241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 3301 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc 3361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 3421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 3481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 3541 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc 3601 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 3661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 3721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 3781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 3841 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3961 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4021 gtttata //