LOCUS BBa_J24735 4028 bp DNA linear 22-Nov-2024 DEFINITION 55. 20(BBa_J24698)==28(BBa_J24708) ACCESSION BBa_J24735 VERSION BBa_J24735.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 misc_feature 2327..2365 /label=LVA CDS 1238..2365 /label=lacI-LVA misc_feature 1238..2365 /label=BBa_C0012 misc_feature 2366..2390 /label=Help:Barcodes CDS 1238..2365 /label=BBa_C0012 misc_feature 1219..2390 /label=BBa_J24684 misc_feature 1..2390 /label=BBa_J24698 misc_feature 2405..2408 /label=conserved RBS 2399..2412 /label=BBa_B0031 CDS 2422..3038 /label=tetR misc_feature 3039..3072 /label=SsrA misc_feature 3079..3103 /label=Help:Barcodes CDS 2419..3103 /label=BBa_C0040 misc_feature 2399..3103 /label=BBa_J24686 misc_feature 3116..3119 /label=conserved RBS 3112..3123 /label=BBa_B0034 CDS 3130..3891 /label=CFP (LVA) misc_feature 3848..3885 /label=SsrA misc_feature 3886..3891 /label=2 misc_feature 3198..3198 /label=A misc_feature 3130..3891 /label=BBa_E0022 misc_feature 3130..3891 /label=BBa_E0022 stem_loop 3911..3954 /label=stem_loop misc_feature 3900..3979 /label=BBa_B0010 terminator 3900..3979 /label=BBa_B0010 stem_loop 3995..4014 /label=T7 TE misc_feature 4021..4021 /label=stop polyA_site 4015..4028 /label=polya misc_feature 3988..4028 /label=BBa_B0012 terminator 3988..4028 /label=BBa_B0012 misc_feature 3112..4028 /label=BBa_J24703 misc_feature 2399..4028 /label=BBa_J24708 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac 1261 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 1321 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 1381 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 1441 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 1501 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 1561 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 1621 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 1681 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 1741 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 1801 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 1861 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 1921 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 1981 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 2041 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 2101 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 2161 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 2221 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 2281 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 2341 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagtc 2401 acacaggaaa cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 2461 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 2521 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 2581 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 2641 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 2701 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 2761 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 2821 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 2881 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 2941 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 3001 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 3061 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag 3121 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 3181 gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 3241 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 3301 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac 3361 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 3421 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 3481 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 3541 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag 3601 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg 3661 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 3721 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 3781 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 3841 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 3901 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3961 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 4021 cgtttata //