##gff-version 3
##sequence-region BBa_J24735 1 4028
BBa_J24735	.	engineered_region	2399	4028	.	+	0	ID=BBa_J24708;Name=BBa_J24708
BBa_J24735	.	engineered_region	2399	3103	.	+	0	ID=BBa_J24686;Name=BBa_J24686;Parent=BBa_J24708
BBa_J24735	.	CDS	2419	3103	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24735	.	tag	3039	3072	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24735	.	engineered_tag	3079	3103	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24735	.	CDS	2422	3038	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24735	.	ribosome_entry_site	2399	2412	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24735	.	conserved_region	2405	2408	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24735	.	engineered_region	3112	4028	.	+	0	ID=BBa_J24703;Name=BBa_J24703;Parent=BBa_J24708
BBa_J24735	.	terminator	3900	3979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24703
BBa_J24735	.	stem_loop	3911	3954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24735	.	engineered_region	3900	3979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24735	.	terminator	3988	4028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24703
BBa_J24735	.	stem_loop	3995	4014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24735	.	polyA_site	4015	4028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24735	.	stop_codon	4021	4021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24735	.	engineered_region	3988	4028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24735	.	engineered_region	3130	3891	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_J24703
BBa_J24735	.	tag	3848	3885	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J24735	.	stop_codon	3886	3891	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J24735	.	engineered_region	3130	3891	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J24735	.	point_mutation	3198	3198	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J24735	.	CDS	3130	3891	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J24735	.	ribosome_entry_site	3112	3123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24703
BBa_J24735	.	conserved_region	3116	3119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24735	.	engineered_region	1	2390	.	+	0	ID=BBa_J24698;Name=BBa_J24698
BBa_J24735	.	engineered_region	1219	2390	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24698
BBa_J24735	.	ribosome_entry_site	1219	1231	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24735	.	ribosome_entry_site	1219	1231	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24735	.	ribosome_entry_site	1225	1228	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24735	.	engineered_region	1219	1231	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24735	.	CDS	1238	2365	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24735	.	engineered_tag	2366	2390	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24735	.	engineered_region	1238	2365	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24735	.	tag	2327	2365	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24735	.	CDS	1238	2365	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24735	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_J24698
BBa_J24735	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J24735	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J24735	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
>BBa_J24735
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgtta
tacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcga
aaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaa
acagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatct
cgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgc
acaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagc
tgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaa
gacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagtt
ctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacggga
aggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctg
gttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcgg
tagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct
ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca
ctggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagc
tggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctg
atagtgctagtgtagatctctactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtaga
gcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacac
tgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccg
gggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcga
tgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacc
accctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgc
ccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcga
gggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctg
gagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatcc
gccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgct
gctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctg
ctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaact
acgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata