LOCUS BBa_J24736 4027 bp DNA linear 22-Dec-2024 DEFINITION 56. 20(BBa_J24698)==29(BBa_J24709) ACCESSION BBa_J24736 VERSION BBa_J24736.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 misc_feature 2327..2365 /label=LVA CDS 1238..2365 /label=lacI-LVA misc_feature 1238..2365 /label=BBa_C0012 misc_feature 2366..2390 /label=Help:Barcodes CDS 1238..2365 /label=BBa_C0012 misc_feature 1219..2390 /label=BBa_J24684 misc_feature 1..2390 /label=BBa_J24698 RBS 2399..2411 /label=RBS-3\Weak RBS 2405..2408 /label=RBS misc_feature 2399..2411 /label=BBa_B0032 RBS 2399..2411 /label=BBa_B0032 CDS 2421..3037 /label=tetR misc_feature 3038..3071 /label=SsrA misc_feature 3078..3102 /label=Help:Barcodes CDS 2418..3102 /label=BBa_C0040 misc_feature 2399..3102 /label=BBa_J24687 misc_feature 3115..3118 /label=conserved RBS 3111..3122 /label=BBa_B0034 CDS 3129..3890 /label=CFP (LVA) misc_feature 3847..3884 /label=SsrA misc_feature 3885..3890 /label=2 misc_feature 3197..3197 /label=A misc_feature 3129..3890 /label=BBa_E0022 misc_feature 3129..3890 /label=BBa_E0022 stem_loop 3910..3953 /label=stem_loop misc_feature 3899..3978 /label=BBa_B0010 terminator 3899..3978 /label=BBa_B0010 stem_loop 3994..4013 /label=T7 TE misc_feature 4020..4020 /label=stop polyA_site 4014..4027 /label=polya misc_feature 3987..4027 /label=BBa_B0012 terminator 3987..4027 /label=BBa_B0012 misc_feature 3111..4027 /label=BBa_J24703 misc_feature 2399..4027 /label=BBa_J24709 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac 1261 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 1321 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 1381 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 1441 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 1501 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 1561 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 1621 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 1681 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 1741 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 1801 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 1861 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 1921 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 1981 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 2041 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 2101 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 2161 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 2221 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 2281 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 2341 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagtc 2401 acacaggaaa gtactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 2461 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 2521 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 2581 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 2641 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 2701 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 2761 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 2821 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 2881 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 2941 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 3001 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 3061 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga 3121 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 3181 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 3241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 3301 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc 3361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 3421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 3481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 3541 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc 3601 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 3661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 3721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 3781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 3841 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3961 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4021 gtttata //