LOCUS BBa_J24737 4026 bp DNA linear 22-Nov-2024 DEFINITION 57. 20(BBa_J24698)==30(BBa_J24710) ACCESSION BBa_J24737 VERSION BBa_J24737.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1231 /label=RBS-3\Weak RBS 1225..1228 /label=RBS misc_feature 1219..1231 /label=BBa_B0032 RBS 1219..1231 /label=BBa_B0032 misc_feature 2327..2365 /label=LVA CDS 1238..2365 /label=lacI-LVA misc_feature 1238..2365 /label=BBa_C0012 misc_feature 2366..2390 /label=Help:Barcodes CDS 1238..2365 /label=BBa_C0012 misc_feature 1219..2390 /label=BBa_J24684 misc_feature 1..2390 /label=BBa_J24698 misc_feature 2403..2406 /label=conserved RBS 2399..2410 /label=BBa_B0034 CDS 2420..3036 /label=tetR misc_feature 3037..3070 /label=SsrA misc_feature 3077..3101 /label=Help:Barcodes CDS 2417..3101 /label=BBa_C0040 misc_feature 2399..3101 /label=BBa_J24688 misc_feature 3114..3117 /label=conserved RBS 3110..3121 /label=BBa_B0034 CDS 3128..3889 /label=CFP (LVA) misc_feature 3846..3883 /label=SsrA misc_feature 3884..3889 /label=2 misc_feature 3196..3196 /label=A misc_feature 3128..3889 /label=BBa_E0022 misc_feature 3128..3889 /label=BBa_E0022 stem_loop 3909..3952 /label=stem_loop misc_feature 3898..3977 /label=BBa_B0010 terminator 3898..3977 /label=BBa_B0010 stem_loop 3993..4012 /label=T7 TE misc_feature 4019..4019 /label=stop polyA_site 4013..4026 /label=polya misc_feature 3986..4026 /label=BBa_B0012 terminator 3986..4026 /label=BBa_B0012 misc_feature 3110..4026 /label=BBa_J24703 misc_feature 2399..4026 /label=BBa_J24710 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac 1261 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 1321 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 1381 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 1441 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 1501 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 1561 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 1621 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 1681 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 1741 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 1801 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 1861 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 1921 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 1981 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 2041 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 2101 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 2161 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 2221 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 2281 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 2341 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa 2401 agaggagaaa tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 2461 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 2521 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 2581 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 2641 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 2701 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 2761 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 2821 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 2881 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 2941 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 3001 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 3061 tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga aagaggagaa 3121 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3181 gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3301 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca 3361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3541 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca 3601 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3841 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca 3901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 4021 tttata //