LOCUS BBa_J24738 4027 bp DNA linear 22-Nov-2024 DEFINITION 58. 21(BBa_J24699)==28(BBa_J24708) ACCESSION BBa_J24738 VERSION BBa_J24738.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 misc_feature 1219..2389 /label=BBa_J24685 misc_feature 1..2389 /label=BBa_J24699 misc_feature 2404..2407 /label=conserved RBS 2398..2411 /label=BBa_B0031 CDS 2421..3037 /label=tetR misc_feature 3038..3071 /label=SsrA misc_feature 3078..3102 /label=Help:Barcodes CDS 2418..3102 /label=BBa_C0040 misc_feature 2398..3102 /label=BBa_J24686 misc_feature 3115..3118 /label=conserved RBS 3111..3122 /label=BBa_B0034 CDS 3129..3890 /label=CFP (LVA) misc_feature 3847..3884 /label=SsrA misc_feature 3885..3890 /label=2 misc_feature 3197..3197 /label=A misc_feature 3129..3890 /label=BBa_E0022 misc_feature 3129..3890 /label=BBa_E0022 stem_loop 3910..3953 /label=stem_loop misc_feature 3899..3978 /label=BBa_B0010 terminator 3899..3978 /label=BBa_B0010 stem_loop 3994..4013 /label=T7 TE misc_feature 4020..4020 /label=stop polyA_site 4014..4027 /label=polya misc_feature 3987..4027 /label=BBa_B0012 terminator 3987..4027 /label=BBa_B0012 misc_feature 3111..4027 /label=BBa_J24703 misc_feature 2398..4027 /label=BBa_J24708 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagtca 2401 cacaggaaac ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 2461 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 2521 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 2581 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 2641 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 2701 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 2761 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 2821 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 2881 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 2941 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 3001 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 3061 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga 3121 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 3181 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 3241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 3301 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc 3361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 3421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 3481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 3541 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc 3601 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 3661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 3721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 3781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 3841 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3901 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3961 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4021 gtttata //