LOCUS BBa_J24739 4026 bp DNA linear 26-Nov-2024 DEFINITION 59. 21(BBa_J24699)==29(BBa_J24709) ACCESSION BBa_J24739 VERSION BBa_J24739.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 misc_feature 1219..2389 /label=BBa_J24685 misc_feature 1..2389 /label=BBa_J24699 RBS 2398..2410 /label=RBS-3\Weak RBS 2404..2407 /label=RBS misc_feature 2398..2410 /label=BBa_B0032 RBS 2398..2410 /label=BBa_B0032 CDS 2420..3036 /label=tetR misc_feature 3037..3070 /label=SsrA misc_feature 3077..3101 /label=Help:Barcodes CDS 2417..3101 /label=BBa_C0040 misc_feature 2398..3101 /label=BBa_J24687 misc_feature 3114..3117 /label=conserved RBS 3110..3121 /label=BBa_B0034 CDS 3128..3889 /label=CFP (LVA) misc_feature 3846..3883 /label=SsrA misc_feature 3884..3889 /label=2 misc_feature 3196..3196 /label=A misc_feature 3128..3889 /label=BBa_E0022 misc_feature 3128..3889 /label=BBa_E0022 stem_loop 3909..3952 /label=stem_loop misc_feature 3898..3977 /label=BBa_B0010 terminator 3898..3977 /label=BBa_B0010 stem_loop 3993..4012 /label=T7 TE misc_feature 4019..4019 /label=stop polyA_site 4013..4026 /label=polya misc_feature 3986..4026 /label=BBa_B0012 terminator 3986..4026 /label=BBa_B0012 misc_feature 3110..4026 /label=BBa_J24703 misc_feature 2398..4026 /label=BBa_J24709 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagtca 2401 cacaggaaag tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 2461 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 2521 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 2581 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 2641 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 2701 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 2761 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 2821 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 2881 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 2941 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 3001 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 3061 tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga aagaggagaa 3121 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3181 gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3301 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca 3361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3541 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca 3601 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3841 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca 3901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 4021 tttata //