##gff-version 3
##sequence-region BBa_J24739 1 4026
BBa_J24739	.	engineered_region	2398	4026	.	+	0	ID=BBa_J24709;Name=BBa_J24709
BBa_J24739	.	engineered_region	2398	3101	.	+	0	ID=BBa_J24687;Name=BBa_J24687;Parent=BBa_J24709
BBa_J24739	.	CDS	2417	3101	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24687
BBa_J24739	.	engineered_tag	3077	3101	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24739	.	CDS	2420	3036	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24739	.	tag	3037	3070	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24739	.	ribosome_entry_site	2398	2410	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24687
BBa_J24739	.	ribosome_entry_site	2398	2410	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24739	.	engineered_region	2398	2410	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24739	.	ribosome_entry_site	2404	2407	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24739	.	engineered_region	3110	4026	.	+	0	ID=BBa_J24703;Name=BBa_J24703;Parent=BBa_J24709
BBa_J24739	.	terminator	3986	4026	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24703
BBa_J24739	.	polyA_site	4013	4026	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24739	.	stop_codon	4019	4019	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24739	.	stem_loop	3993	4012	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24739	.	engineered_region	3986	4026	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24739	.	ribosome_entry_site	3110	3121	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24703
BBa_J24739	.	conserved_region	3114	3117	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24739	.	engineered_region	3128	3889	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_J24703
BBa_J24739	.	tag	3846	3883	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J24739	.	engineered_region	3128	3889	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J24739	.	stop_codon	3884	3889	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J24739	.	CDS	3128	3889	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J24739	.	point_mutation	3196	3196	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J24739	.	terminator	3898	3977	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24703
BBa_J24739	.	stem_loop	3909	3952	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24739	.	engineered_region	3898	3977	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24739	.	engineered_region	1	2389	.	+	0	ID=BBa_J24699;Name=BBa_J24699
BBa_J24739	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_J24699
BBa_J24739	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J24739	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J24739	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J24739	.	engineered_region	1219	2389	.	+	0	ID=BBa_J24685;Name=BBa_J24685;Parent=BBa_J24699
BBa_J24739	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24739	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24739	.	CDS	1237	2364	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24739	.	engineered_tag	2365	2389	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24739	.	CDS	1237	2364	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24739	.	engineered_region	1237	2364	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24739	.	tag	2326	2364	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_J24739
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttat
acgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaa
aacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaa
cagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctc
gcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgca
caatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagct
gcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaag
acggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttc
tgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaa
ggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctgg
ttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggt
agtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctg
gggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcac
tggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctga
tagtgctagtgtagatctctactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaac
agcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagc
agcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcacca
tactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcttta
ctaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaat
tagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttagg
ttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccatta
ttacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcg
gattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactg
atagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggg
gtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatg
ccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccac
cctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgccc
gaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagg
gcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgga
gtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgc
cacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgc
tgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgct
ggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactac
gctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata