LOCUS BBa_J24741 5903 bp DNA linear 22-Nov-2024 DEFINITION 61. 34(BBa_J24714)== 43(BBa_J24723) ACCESSION BBa_J24741 VERSION BBa_J24741.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 misc_feature 1183..1186 /label=conserved RBS 1177..1190 /label=BBa_B0031 CDS 1200..1816 /label=tetR misc_feature 1817..1850 /label=SsrA misc_feature 1857..1881 /label=Help:Barcodes CDS 1197..1881 /label=BBa_C0040 misc_feature 1177..1881 /label=BBa_J24686 misc_feature 1894..1897 /label=conserved RBS 1890..1901 /label=BBa_B0034 CDS 1908..2615 /label=mCherry misc_feature 2259..2259 /label=C->T (removing PstI site) misc_feature 2616..2624 /label=LVA misc_feature 1908..2630 /label=BBa_J06505 stem_loop 2650..2693 /label=stem_loop misc_feature 2639..2718 /label=BBa_B0010 terminator 2639..2718 /label=BBa_B0010 stem_loop 2734..2753 /label=T7 TE misc_feature 2760..2760 /label=stop polyA_site 2754..2767 /label=polya misc_feature 2727..2767 /label=BBa_B0012 terminator 2727..2767 /label=BBa_B0012 misc_feature 1890..2767 /label=BBa_J24702 misc_feature 1177..2767 /label=BBa_J24705 misc_feature 1..2767 /label=BBa_J24714 misc_feature 2776..2794 /label=TetR 1 misc_feature 2776..2829 /label=BBa_R0040 promoter 2795..2800 /label=-35 misc_feature 2801..2819 /label=TetR 2 promoter 2818..2823 /label=-10 promoter 2776..2829 /label=BBa_R0040 misc_feature 2844..2847 /label=conserved RBS 2838..2851 /label=BBa_B0031 CDS 2858..3733 /label=araC misc_feature 3734..3766 /label=LVA misc_feature 3773..3797 /label=Help:Barcodes CDS 2858..3797 /label=BBa_C0080 misc_feature 2776..3797 /label=BBa_J24694 misc_feature 3812..3815 /label=conserved RBS 3806..3819 /label=BBa_B0031 misc_feature 4915..4953 /label=LVA CDS 3826..4953 /label=lacI-LVA misc_feature 3826..4953 /label=BBa_C0012 misc_feature 4954..4978 /label=Help:Barcodes CDS 3826..4953 /label=BBa_C0012 misc_feature 3806..4978 /label=BBa_J24683 misc_feature 4991..4994 /label=conserved RBS 4987..4998 /label=BBa_B0034 CDS 5005..5766 /label=YFP (LVA) misc_feature 5761..5766 /label=2 misc_feature 5722..5760 /label=SsrA misc_feature 5246..5246 /label=A (Q->L) misc_feature 5005..5766 /label=BBa_E0032 misc_feature 5005..5766 /label=BBa_E0032 stem_loop 5786..5829 /label=stem_loop misc_feature 5775..5854 /label=BBa_B0010 terminator 5775..5854 /label=BBa_B0010 stem_loop 5870..5889 /label=T7 TE misc_feature 5896..5896 /label=stop polyA_site 5890..5903 /label=polya misc_feature 5863..5903 /label=BBa_B0012 terminator 5863..5903 /label=BBa_B0012 misc_feature 4987..5903 /label=BBa_J24704 misc_feature 3806..5903 /label=BBa_J24711 misc_feature 2776..5903 /label=BBa_J24723 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaacc tactagatgt 1201 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 1261 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 1321 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 1381 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 1441 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 1501 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 1561 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 1621 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 1681 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 1741 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 1801 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 1861 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg 1921 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 1981 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 2041 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 2101 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 2161 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 2221 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct 2281 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 2341 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 2401 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 2461 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 2521 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 2581 gccactccac cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagcc 2641 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2701 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2761 gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat 2821 actgagcact actagagtca cacaggaaac ctactagatg gctgaagcgc aaaatgatcc 2881 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc 2941 caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa 3001 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc 3061 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc 3121 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct 3181 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc 3241 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc 3301 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa 3361 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga 3421 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc 3481 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga 3541 ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac 3601 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg 3661 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc 3721 cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 3781 gtgctagtgt agatcactac tagagtcaca caggaaacct actagatggt gaatgtgaaa 3841 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 3901 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 3961 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 4021 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 4081 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 4141 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 4201 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 4261 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 4321 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 4381 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 4441 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 4501 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 4561 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 4621 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 4681 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 4741 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 4801 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 4861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca 4921 aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta 4981 ctagagaaag aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg 5041 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 5101 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 5161 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc 5221 gcccgctacc ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc 5281 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 5341 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 5401 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 5461 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 5521 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 5581 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc 5641 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 5701 ggcatggacg agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct 5761 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 5821 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 5881 gggtgggcct ttctgcgttt ata //