LOCUS BBa_J24742 5898 bp DNA linear 28-Dec-2024 DEFINITION 75. 38(BBa_J24718)==48(BBa_J24728) ACCESSION BBa_J24742 VERSION BBa_J24742.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 RBS 1176..1188 /label=RBS-3\Weak RBS 1182..1185 /label=RBS misc_feature 1176..1188 /label=BBa_B0032 RBS 1176..1188 /label=BBa_B0032 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1176..1879 /label=BBa_J24687 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1176..2765 /label=BBa_J24706 misc_feature 1..2765 /label=BBa_J24718 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 RBS 2836..2848 /label=RBS-3\Weak RBS 2842..2845 /label=RBS misc_feature 2836..2848 /label=BBa_B0032 RBS 2836..2848 /label=BBa_B0032 CDS 2855..3730 /label=araC misc_feature 3731..3763 /label=LVA misc_feature 3770..3794 /label=Help:Barcodes CDS 2855..3794 /label=BBa_C0080 misc_feature 2774..3794 /label=BBa_J24695 misc_feature 3807..3810 /label=conserved RBS 3803..3814 /label=BBa_B0034 misc_feature 4910..4948 /label=LVA CDS 3821..4948 /label=lacI-LVA misc_feature 3821..4948 /label=BBa_C0012 misc_feature 4949..4973 /label=Help:Barcodes CDS 3821..4948 /label=BBa_C0012 misc_feature 3803..4973 /label=BBa_J24685 misc_feature 4986..4989 /label=conserved RBS 4982..4993 /label=BBa_B0034 CDS 5000..5761 /label=YFP (LVA) misc_feature 5756..5761 /label=2 misc_feature 5717..5755 /label=SsrA misc_feature 5241..5241 /label=A (Q->L) misc_feature 5000..5761 /label=BBa_E0032 misc_feature 5000..5761 /label=BBa_E0032 stem_loop 5781..5824 /label=stem_loop misc_feature 5770..5849 /label=BBa_B0010 terminator 5770..5849 /label=BBa_B0010 stem_loop 5865..5884 /label=T7 TE misc_feature 5891..5891 /label=stop polyA_site 5885..5898 /label=polya misc_feature 5858..5898 /label=BBa_B0012 terminator 5858..5898 /label=BBa_B0012 misc_feature 4982..5898 /label=BBa_J24704 misc_feature 3803..5898 /label=BBa_J24713 misc_feature 2774..5898 /label=BBa_J24728 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaagta ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagtcaca caggaaagta ctagatggct gaagcgcaaa atgatcccct 2881 gctgccggga tactcgttta acgcccatct ggtggcgggt ttaacgccga ttgaggccaa 2941 tggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata ttctcaatct 3001 caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgtct gccgaccggg 3061 tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc cggaggctcg 3121 cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg aatggcttaa 3181 ctggccgtca atatttgcca atacgggttt ctttcgcccg gatgaagcgc accagccgca 3241 tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc gctattcgga 3301 gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag cgattaacga 3361 gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca tcagcgatca 3421 cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgcc tgtcgccgtc 3481 gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga 3541 ccaacgcatc agccaggcga agctgctttt gagcactacc cggatgccta tcgccaccgt 3601 cggtcgcaat gttggttttg acgatcaact ctatttctcg cgagtattta aaaaatgcac 3661 cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt 3721 caagttgtca gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg 3781 ctagtgtaga tcactactag agaaagagga gaaatactag atggtgaatg tgaaaccagt 3841 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 3901 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 3961 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 4021 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 4081 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 4141 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 4201 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 4261 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 4321 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 4381 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 4441 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 4501 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 4561 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 4621 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 4681 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 4741 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 4801 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 4861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga 4921 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 4981 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 5041 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 5101 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 5161 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 5221 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 5281 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 5341 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 5401 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 5461 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 5521 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 5581 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 5641 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 5701 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 5761 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 5821 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 5881 ggcctttctg cgtttata //