LOCUS BBa_J24743 5902 bp DNA linear 18-Feb-2025 DEFINITION 62. 34(BBa_J24714)==44(BBa_J24724) ACCESSION BBa_J24743 VERSION BBa_J24743.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 misc_feature 1183..1186 /label=conserved RBS 1177..1190 /label=BBa_B0031 CDS 1200..1816 /label=tetR misc_feature 1817..1850 /label=SsrA misc_feature 1857..1881 /label=Help:Barcodes CDS 1197..1881 /label=BBa_C0040 misc_feature 1177..1881 /label=BBa_J24686 misc_feature 1894..1897 /label=conserved RBS 1890..1901 /label=BBa_B0034 CDS 1908..2615 /label=mCherry misc_feature 2259..2259 /label=C->T (removing PstI site) misc_feature 2616..2624 /label=LVA misc_feature 1908..2630 /label=BBa_J06505 stem_loop 2650..2693 /label=stem_loop misc_feature 2639..2718 /label=BBa_B0010 terminator 2639..2718 /label=BBa_B0010 stem_loop 2734..2753 /label=T7 TE misc_feature 2760..2760 /label=stop polyA_site 2754..2767 /label=polya misc_feature 2727..2767 /label=BBa_B0012 terminator 2727..2767 /label=BBa_B0012 misc_feature 1890..2767 /label=BBa_J24702 misc_feature 1177..2767 /label=BBa_J24705 misc_feature 1..2767 /label=BBa_J24714 misc_feature 2776..2794 /label=TetR 1 misc_feature 2776..2829 /label=BBa_R0040 promoter 2795..2800 /label=-35 misc_feature 2801..2819 /label=TetR 2 promoter 2818..2823 /label=-10 promoter 2776..2829 /label=BBa_R0040 misc_feature 2844..2847 /label=conserved RBS 2838..2851 /label=BBa_B0031 CDS 2858..3733 /label=araC misc_feature 3734..3766 /label=LVA misc_feature 3773..3797 /label=Help:Barcodes CDS 2858..3797 /label=BBa_C0080 misc_feature 2776..3797 /label=BBa_J24694 RBS 3806..3818 /label=RBS-3\Weak RBS 3812..3815 /label=RBS misc_feature 3806..3818 /label=BBa_B0032 RBS 3806..3818 /label=BBa_B0032 misc_feature 4914..4952 /label=LVA CDS 3825..4952 /label=lacI-LVA misc_feature 3825..4952 /label=BBa_C0012 misc_feature 4953..4977 /label=Help:Barcodes CDS 3825..4952 /label=BBa_C0012 misc_feature 3806..4977 /label=BBa_J24684 misc_feature 4990..4993 /label=conserved RBS 4986..4997 /label=BBa_B0034 CDS 5004..5765 /label=YFP (LVA) misc_feature 5760..5765 /label=2 misc_feature 5721..5759 /label=SsrA misc_feature 5245..5245 /label=A (Q->L) misc_feature 5004..5765 /label=BBa_E0032 misc_feature 5004..5765 /label=BBa_E0032 stem_loop 5785..5828 /label=stem_loop misc_feature 5774..5853 /label=BBa_B0010 terminator 5774..5853 /label=BBa_B0010 stem_loop 5869..5888 /label=T7 TE misc_feature 5895..5895 /label=stop polyA_site 5889..5902 /label=polya misc_feature 5862..5902 /label=BBa_B0012 terminator 5862..5902 /label=BBa_B0012 misc_feature 4986..5902 /label=BBa_J24704 misc_feature 3806..5902 /label=BBa_J24712 misc_feature 2776..5902 /label=BBa_J24724 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaacc tactagatgt 1201 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 1261 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 1321 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 1381 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 1441 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 1501 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 1561 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 1621 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 1681 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 1741 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 1801 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 1861 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg 1921 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 1981 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 2041 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 2101 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 2161 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 2221 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct 2281 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 2341 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 2401 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 2461 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 2521 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 2581 gccactccac cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagcc 2641 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2701 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2761 gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat 2821 actgagcact actagagtca cacaggaaac ctactagatg gctgaagcgc aaaatgatcc 2881 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc 2941 caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa 3001 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc 3061 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc 3121 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct 3181 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc 3241 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc 3301 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa 3361 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga 3421 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc 3481 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga 3541 ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac 3601 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg 3661 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc 3721 cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 3781 gtgctagtgt agatcactac tagagtcaca caggaaagta ctagatggtg aatgtgaaac 3841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 3901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 3961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 4021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 4081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 4141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 4201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 4261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 4321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 4381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 4441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 4501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 4561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 4621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 4681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 4741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 4801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 4861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa 4921 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac 4981 tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg 5041 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 5101 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 5161 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg 5221 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct 5281 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 5341 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 5401 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 5461 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 5521 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 5581 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 5641 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 5701 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt 5761 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 5821 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 5881 ggtgggcctt tctgcgttta ta //