LOCUS BBa_J24744 5901 bp DNA linear 22-Dec-2024 DEFINITION 63. 34(BBa_J24714)==45(BBa_J24725) ACCESSION BBa_J24744 VERSION BBa_J24744.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 misc_feature 1183..1186 /label=conserved RBS 1177..1190 /label=BBa_B0031 CDS 1200..1816 /label=tetR misc_feature 1817..1850 /label=SsrA misc_feature 1857..1881 /label=Help:Barcodes CDS 1197..1881 /label=BBa_C0040 misc_feature 1177..1881 /label=BBa_J24686 misc_feature 1894..1897 /label=conserved RBS 1890..1901 /label=BBa_B0034 CDS 1908..2615 /label=mCherry misc_feature 2259..2259 /label=C->T (removing PstI site) misc_feature 2616..2624 /label=LVA misc_feature 1908..2630 /label=BBa_J06505 stem_loop 2650..2693 /label=stem_loop misc_feature 2639..2718 /label=BBa_B0010 terminator 2639..2718 /label=BBa_B0010 stem_loop 2734..2753 /label=T7 TE misc_feature 2760..2760 /label=stop polyA_site 2754..2767 /label=polya misc_feature 2727..2767 /label=BBa_B0012 terminator 2727..2767 /label=BBa_B0012 misc_feature 1890..2767 /label=BBa_J24702 misc_feature 1177..2767 /label=BBa_J24705 misc_feature 1..2767 /label=BBa_J24714 misc_feature 2776..2794 /label=TetR 1 misc_feature 2776..2829 /label=BBa_R0040 promoter 2795..2800 /label=-35 misc_feature 2801..2819 /label=TetR 2 promoter 2818..2823 /label=-10 promoter 2776..2829 /label=BBa_R0040 misc_feature 2844..2847 /label=conserved RBS 2838..2851 /label=BBa_B0031 CDS 2858..3733 /label=araC misc_feature 3734..3766 /label=LVA misc_feature 3773..3797 /label=Help:Barcodes CDS 2858..3797 /label=BBa_C0080 misc_feature 2776..3797 /label=BBa_J24694 misc_feature 3810..3813 /label=conserved RBS 3806..3817 /label=BBa_B0034 misc_feature 4913..4951 /label=LVA CDS 3824..4951 /label=lacI-LVA misc_feature 3824..4951 /label=BBa_C0012 misc_feature 4952..4976 /label=Help:Barcodes CDS 3824..4951 /label=BBa_C0012 misc_feature 3806..4976 /label=BBa_J24685 misc_feature 4989..4992 /label=conserved RBS 4985..4996 /label=BBa_B0034 CDS 5003..5764 /label=YFP (LVA) misc_feature 5759..5764 /label=2 misc_feature 5720..5758 /label=SsrA misc_feature 5244..5244 /label=A (Q->L) misc_feature 5003..5764 /label=BBa_E0032 misc_feature 5003..5764 /label=BBa_E0032 stem_loop 5784..5827 /label=stem_loop misc_feature 5773..5852 /label=BBa_B0010 terminator 5773..5852 /label=BBa_B0010 stem_loop 5868..5887 /label=T7 TE misc_feature 5894..5894 /label=stop polyA_site 5888..5901 /label=polya misc_feature 5861..5901 /label=BBa_B0012 terminator 5861..5901 /label=BBa_B0012 misc_feature 4985..5901 /label=BBa_J24704 misc_feature 3806..5901 /label=BBa_J24713 misc_feature 2776..5901 /label=BBa_J24725 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaacc tactagatgt 1201 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 1261 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 1321 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 1381 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 1441 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 1501 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 1561 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 1621 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 1681 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 1741 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 1801 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 1861 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg 1921 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg 1981 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 2041 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 2101 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 2161 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 2221 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct 2281 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 2341 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 2401 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca 2461 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg 2521 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc 2581 gccactccac cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagcc 2641 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 2701 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 2761 gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat 2821 actgagcact actagagtca cacaggaaac ctactagatg gctgaagcgc aaaatgatcc 2881 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc 2941 caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa 3001 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc 3061 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc 3121 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct 3181 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc 3241 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc 3301 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa 3361 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga 3421 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc 3481 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga 3541 ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac 3601 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg 3661 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc 3721 cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 3781 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga atgtgaaacc 3841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 3901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 3961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 4921 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 4981 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 5041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 5101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 5161 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 5221 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 5281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 5341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 5401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 5461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 5521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 5581 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 5641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 5701 catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta 5761 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 5821 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 5881 gtgggccttt ctgcgtttat a //