##gff-version 3
##sequence-region BBa_J24744 1 5901
BBa_J24744	.	engineered_region	2776	5901	.	+	0	ID=BBa_J24725;Name=BBa_J24725
BBa_J24744	.	engineered_region	2776	3797	.	+	0	ID=BBa_J24694;Name=BBa_J24694;Parent=BBa_J24725
BBa_J24744	.	ribosome_entry_site	2838	2851	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24694
BBa_J24744	.	conserved_region	2844	2847	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24744	.	promoter	2776	2829	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24694
BBa_J24744	.	engineered_region	2776	2829	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24744	.	promoter	2795	2800	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24744	.	non_covalent_binding_site	2801	2819	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24744	.	promoter	2818	2823	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24744	.	non_covalent_binding_site	2776	2794	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24744	.	CDS	2858	3797	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24694
BBa_J24744	.	CDS	2858	3733	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24744	.	engineered_tag	3773	3797	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24744	.	tag	3734	3766	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24744	.	engineered_region	3806	5901	.	+	0	ID=BBa_J24713;Name=BBa_J24713;Parent=BBa_J24725
BBa_J24744	.	engineered_region	4985	5901	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24713
BBa_J24744	.	engineered_region	5003	5764	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24744	.	CDS	5003	5764	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24744	.	stop_codon	5759	5764	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24744	.	engineered_region	5003	5764	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24744	.	tag	5720	5758	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24744	.	sequence_alteration	5244	5244	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24744	.	terminator	5773	5852	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24744	.	stem_loop	5784	5827	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24744	.	engineered_region	5773	5852	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24744	.	ribosome_entry_site	4985	4996	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24744	.	conserved_region	4989	4992	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24744	.	terminator	5861	5901	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24744	.	stem_loop	5868	5887	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24744	.	stop_codon	5894	5894	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24744	.	engineered_region	5861	5901	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24744	.	polyA_site	5888	5901	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24744	.	engineered_region	3806	4976	.	+	0	ID=BBa_J24685;Name=BBa_J24685;Parent=BBa_J24713
BBa_J24744	.	CDS	3824	4951	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24744	.	engineered_region	3824	4951	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24744	.	engineered_tag	4952	4976	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24744	.	tag	4913	4951	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24744	.	CDS	3824	4951	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24744	.	ribosome_entry_site	3806	3817	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24744	.	conserved_region	3810	3813	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24744	.	engineered_region	1	2767	.	+	0	ID=BBa_J24714;Name=BBa_J24714
BBa_J24744	.	engineered_region	1177	2767	.	+	0	ID=BBa_J24705;Name=BBa_J24705;Parent=BBa_J24714
BBa_J24744	.	engineered_region	1177	1881	.	+	0	ID=BBa_J24686;Name=BBa_J24686;Parent=BBa_J24705
BBa_J24744	.	CDS	1197	1881	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24744	.	engineered_tag	1857	1881	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24744	.	tag	1817	1850	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24744	.	CDS	1200	1816	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24744	.	ribosome_entry_site	1177	1190	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24744	.	conserved_region	1183	1186	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24744	.	engineered_region	1890	2767	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24705
BBa_J24744	.	terminator	2727	2767	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24744	.	stem_loop	2734	2753	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24744	.	stop_codon	2760	2760	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24744	.	engineered_region	2727	2767	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24744	.	polyA_site	2754	2767	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24744	.	terminator	2639	2718	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24744	.	stem_loop	2650	2693	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24744	.	engineered_region	2639	2718	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24744	.	ribosome_entry_site	1890	1901	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24744	.	conserved_region	1894	1897	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24744	.	engineered_region	1908	2630	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24744	.	sequence_alteration	2259	2259	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24744	.	CDS	1908	2615	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24744	.	tag	2616	2624	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24744	.	engineered_region	1	1168	.	+	0	ID=BBa_J24691;Name=BBa_J24691;Parent=BBa_J24714
BBa_J24744	.	CDS	229	1168	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24691
BBa_J24744	.	CDS	229	1104	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24744	.	engineered_tag	1144	1168	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24744	.	tag	1105	1137	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24744	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24691
BBa_J24744	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24744	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24691
BBa_J24744	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24744	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24744	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24744	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24744	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24744	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24744	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_J24744
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggctgaa
gcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatg
gttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggt
ggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactac
ggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggctta
actggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgg
gcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactg
cggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcg
atcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatct
tttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagc
actacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaa
aatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagg
aaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga
aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg
gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc
aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg
tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag
aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta
aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg
tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacat
ggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcc
aagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaagg
cctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgat
gaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaag
ctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcgga
tgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctga
ggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacaca
ggaaacctactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggt
ttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatc
tcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttccc
gccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgc
gcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagc
cgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaa
tctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgc
gaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgt
cgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcag
ccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactc
tatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatg
atgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtag
atcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgcc
ggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaag
cggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgt
tgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgcc
agcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcg
tcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggc
gttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtg
gagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtc
tggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtc
cggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcg
ctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccg
aagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccg
cttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccacc
ctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctct
actagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgag
ctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccc
tgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatg
cttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgc
accatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgca
tcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaa
cgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagc
gtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctga
gctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgg
gatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata