LOCUS BBa_J24745 5902 bp DNA linear 26-Nov-2024 DEFINITION 64. 35(BBa_J24715)==43(BBa_J24723) ACCESSION BBa_J24745 VERSION BBa_J24745.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 RBS 1177..1189 /label=RBS-3\Weak RBS 1183..1186 /label=RBS misc_feature 1177..1189 /label=BBa_B0032 RBS 1177..1189 /label=BBa_B0032 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1177..1880 /label=BBa_J24687 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1177..2766 /label=BBa_J24706 misc_feature 1..2766 /label=BBa_J24715 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 misc_feature 2843..2846 /label=conserved RBS 2837..2850 /label=BBa_B0031 CDS 2857..3732 /label=araC misc_feature 3733..3765 /label=LVA misc_feature 3772..3796 /label=Help:Barcodes CDS 2857..3796 /label=BBa_C0080 misc_feature 2775..3796 /label=BBa_J24694 misc_feature 3811..3814 /label=conserved RBS 3805..3818 /label=BBa_B0031 misc_feature 4914..4952 /label=LVA CDS 3825..4952 /label=lacI-LVA misc_feature 3825..4952 /label=BBa_C0012 misc_feature 4953..4977 /label=Help:Barcodes CDS 3825..4952 /label=BBa_C0012 misc_feature 3805..4977 /label=BBa_J24683 misc_feature 4990..4993 /label=conserved RBS 4986..4997 /label=BBa_B0034 CDS 5004..5765 /label=YFP (LVA) misc_feature 5760..5765 /label=2 misc_feature 5721..5759 /label=SsrA misc_feature 5245..5245 /label=A (Q->L) misc_feature 5004..5765 /label=BBa_E0032 misc_feature 5004..5765 /label=BBa_E0032 stem_loop 5785..5828 /label=stem_loop misc_feature 5774..5853 /label=BBa_B0010 terminator 5774..5853 /label=BBa_B0010 stem_loop 5869..5888 /label=T7 TE misc_feature 5895..5895 /label=stop polyA_site 5889..5902 /label=polya misc_feature 5862..5902 /label=BBa_B0012 terminator 5862..5902 /label=BBa_B0012 misc_feature 4986..5902 /label=BBa_J24704 misc_feature 3805..5902 /label=BBa_J24711 misc_feature 2775..5902 /label=BBa_J24723 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaacc tactagatgg ctgaagcgca aaatgatccc 2881 ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc gattgaggcc 2941 aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat 3001 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ctgccgaccg 3061 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct 3121 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt 3181 aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc gcaccagccg 3241 catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg gcgctattcg 3301 gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga agcgattaac 3361 gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta catcagcgat 3421 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cctgtcgccg 3481 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag 3541 gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc tatcgccacc 3601 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt taaaaaatgc 3661 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc 3721 gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 3781 tgctagtgta gatcactact agagtcacac aggaaaccta ctagatggtg aatgtgaaac 3841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 3901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 3961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 4021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 4081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 4141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 4201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 4261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 4321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 4381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 4441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 4501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 4561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 4621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 4681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 4741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 4801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 4861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa 4921 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac 4981 tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg 5041 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 5101 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 5161 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg 5221 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct 5281 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 5341 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 5401 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 5461 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 5521 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 5581 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 5641 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 5701 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt 5761 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 5821 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 5881 ggtgggcctt tctgcgttta ta //