##gff-version 3
##sequence-region BBa_J24745 1 5902
BBa_J24745	.	engineered_region	1	2766	.	+	0	ID=BBa_J24715;Name=BBa_J24715
BBa_J24745	.	engineered_region	1	1168	.	+	0	ID=BBa_J24691;Name=BBa_J24691;Parent=BBa_J24715
BBa_J24745	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24691
BBa_J24745	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24745	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24745	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24745	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24745	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24745	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24745	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24745	.	CDS	229	1168	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24691
BBa_J24745	.	engineered_tag	1144	1168	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24745	.	CDS	229	1104	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24745	.	tag	1105	1137	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24745	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24691
BBa_J24745	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24745	.	engineered_region	1177	2766	.	+	0	ID=BBa_J24706;Name=BBa_J24706;Parent=BBa_J24715
BBa_J24745	.	engineered_region	1889	2766	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24706
BBa_J24745	.	terminator	2726	2766	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24745	.	polyA_site	2753	2766	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24745	.	stop_codon	2759	2759	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24745	.	engineered_region	2726	2766	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24745	.	stem_loop	2733	2752	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24745	.	engineered_region	1907	2629	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24745	.	tag	2615	2623	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24745	.	CDS	1907	2614	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24745	.	sequence_alteration	2258	2258	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24745	.	ribosome_entry_site	1889	1900	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24745	.	conserved_region	1893	1896	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24745	.	terminator	2638	2717	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24745	.	engineered_region	2638	2717	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24745	.	stem_loop	2649	2692	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24745	.	engineered_region	1177	1880	.	+	0	ID=BBa_J24687;Name=BBa_J24687;Parent=BBa_J24706
BBa_J24745	.	CDS	1196	1880	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24687
BBa_J24745	.	tag	1816	1849	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24745	.	engineered_tag	1856	1880	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24745	.	CDS	1199	1815	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24745	.	ribosome_entry_site	1177	1189	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24687
BBa_J24745	.	engineered_region	1177	1189	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24745	.	ribosome_entry_site	1177	1189	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24745	.	ribosome_entry_site	1183	1186	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24745	.	engineered_region	2775	5902	.	+	0	ID=BBa_J24723;Name=BBa_J24723
BBa_J24745	.	engineered_region	2775	3796	.	+	0	ID=BBa_J24694;Name=BBa_J24694;Parent=BBa_J24723
BBa_J24745	.	ribosome_entry_site	2837	2850	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24694
BBa_J24745	.	conserved_region	2843	2846	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24745	.	CDS	2857	3796	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24694
BBa_J24745	.	engineered_tag	3772	3796	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24745	.	CDS	2857	3732	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24745	.	tag	3733	3765	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24745	.	promoter	2775	2828	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24694
BBa_J24745	.	non_covalent_binding_site	2800	2818	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24745	.	promoter	2817	2822	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24745	.	promoter	2794	2799	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24745	.	engineered_region	2775	2828	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24745	.	non_covalent_binding_site	2775	2793	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24745	.	engineered_region	3805	5902	.	+	0	ID=BBa_J24711;Name=BBa_J24711;Parent=BBa_J24723
BBa_J24745	.	engineered_region	3805	4977	.	+	0	ID=BBa_J24683;Name=BBa_J24683;Parent=BBa_J24711
BBa_J24745	.	ribosome_entry_site	3805	3818	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24683
BBa_J24745	.	conserved_region	3811	3814	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24745	.	CDS	3825	4952	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24683
BBa_J24745	.	engineered_region	3825	4952	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24745	.	tag	4914	4952	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24745	.	engineered_tag	4953	4977	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24745	.	CDS	3825	4952	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24745	.	engineered_region	4986	5902	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24711
BBa_J24745	.	terminator	5774	5853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24745	.	engineered_region	5774	5853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24745	.	stem_loop	5785	5828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24745	.	terminator	5862	5902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24745	.	polyA_site	5889	5902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24745	.	stop_codon	5895	5895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24745	.	engineered_region	5862	5902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24745	.	stem_loop	5869	5888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24745	.	engineered_region	5004	5765	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24745	.	stop_codon	5760	5765	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24745	.	tag	5721	5759	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24745	.	engineered_region	5004	5765	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24745	.	CDS	5004	5765	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24745	.	sequence_alteration	5245	5245	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24745	.	ribosome_entry_site	4986	4997	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24745	.	conserved_region	4990	4993	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J24745
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggctgaa
gcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatg
gttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggt
ggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactac
ggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggctta
actggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgg
gcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactg
cggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcg
atcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatct
tttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagc
actacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaa
aatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagg
aaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaa
ggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcggg
ctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggca
agattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggt
acacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagaga
atgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa
agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggt
gcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccg
ctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagga
gaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatg
gagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcca
agctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggc
ctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatg
aacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagc
tgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggat
gtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgag
gtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacct
cccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagct
gtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacag
gaaacctactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtt
taacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatct
caccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccg
ccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcg
cctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagcc
gcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaat
ctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcg
aggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtc
gccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagc
caggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactct
atttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatga
tgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtaga
tcactactagagtcacacaggaaacctactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgc
cggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaa
gcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcg
ttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc
cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgc
gtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccgg
cgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgt
ggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgt
ctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgt
ccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggc
gctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatacc
gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggacc
gcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac
cctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccga
ctggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
tactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcga
gctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgacc
ctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaat
gcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcg
caccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgc
atcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccaca
acgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcag
cgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctg
agctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccg
ggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata