LOCUS BBa_J24746 5901 bp DNA linear 23-Dec-2024 DEFINITION 65. 35(BBa_J24715)==44(BBa_J24724) ACCESSION BBa_J24746 VERSION BBa_J24746.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 RBS 1177..1189 /label=RBS-3\Weak RBS 1183..1186 /label=RBS misc_feature 1177..1189 /label=BBa_B0032 RBS 1177..1189 /label=BBa_B0032 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1177..1880 /label=BBa_J24687 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1177..2766 /label=BBa_J24706 misc_feature 1..2766 /label=BBa_J24715 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 misc_feature 2843..2846 /label=conserved RBS 2837..2850 /label=BBa_B0031 CDS 2857..3732 /label=araC misc_feature 3733..3765 /label=LVA misc_feature 3772..3796 /label=Help:Barcodes CDS 2857..3796 /label=BBa_C0080 misc_feature 2775..3796 /label=BBa_J24694 RBS 3805..3817 /label=RBS-3\Weak RBS 3811..3814 /label=RBS misc_feature 3805..3817 /label=BBa_B0032 RBS 3805..3817 /label=BBa_B0032 misc_feature 4913..4951 /label=LVA CDS 3824..4951 /label=lacI-LVA misc_feature 3824..4951 /label=BBa_C0012 misc_feature 4952..4976 /label=Help:Barcodes CDS 3824..4951 /label=BBa_C0012 misc_feature 3805..4976 /label=BBa_J24684 misc_feature 4989..4992 /label=conserved RBS 4985..4996 /label=BBa_B0034 CDS 5003..5764 /label=YFP (LVA) misc_feature 5759..5764 /label=2 misc_feature 5720..5758 /label=SsrA misc_feature 5244..5244 /label=A (Q->L) misc_feature 5003..5764 /label=BBa_E0032 misc_feature 5003..5764 /label=BBa_E0032 stem_loop 5784..5827 /label=stem_loop misc_feature 5773..5852 /label=BBa_B0010 terminator 5773..5852 /label=BBa_B0010 stem_loop 5868..5887 /label=T7 TE misc_feature 5894..5894 /label=stop polyA_site 5888..5901 /label=polya misc_feature 5861..5901 /label=BBa_B0012 terminator 5861..5901 /label=BBa_B0012 misc_feature 4985..5901 /label=BBa_J24704 misc_feature 3805..5901 /label=BBa_J24712 misc_feature 2775..5901 /label=BBa_J24724 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaacc tactagatgg ctgaagcgca aaatgatccc 2881 ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc gattgaggcc 2941 aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat 3001 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ctgccgaccg 3061 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct 3121 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt 3181 aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc gcaccagccg 3241 catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg gcgctattcg 3301 gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga agcgattaac 3361 gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta catcagcgat 3421 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cctgtcgccg 3481 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag 3541 gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc tatcgccacc 3601 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt taaaaaatgc 3661 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc 3721 gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 3781 tgctagtgta gatcactact agagtcacac aggaaagtac tagatggtga atgtgaaacc 3841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 3901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 3961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 4921 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 4981 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 5041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 5101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 5161 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 5221 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 5281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 5341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 5401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 5461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 5521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 5581 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 5641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 5701 catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta 5761 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 5821 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 5881 gtgggccttt ctgcgtttat a //