##gff-version 3
##sequence-region BBa_J24749 1 5900
BBa_J24749	.	engineered_region	2774	5900	.	+	0	ID=BBa_J24724;Name=BBa_J24724
BBa_J24749	.	engineered_region	2774	3795	.	+	0	ID=BBa_J24694;Name=BBa_J24694;Parent=BBa_J24724
BBa_J24749	.	promoter	2774	2827	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24694
BBa_J24749	.	non_covalent_binding_site	2774	2792	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24749	.	promoter	2793	2798	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24749	.	non_covalent_binding_site	2799	2817	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24749	.	engineered_region	2774	2827	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24749	.	promoter	2816	2821	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24749	.	CDS	2856	3795	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24694
BBa_J24749	.	tag	3732	3764	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24749	.	CDS	2856	3731	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24749	.	engineered_tag	3771	3795	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24749	.	ribosome_entry_site	2836	2849	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24694
BBa_J24749	.	conserved_region	2842	2845	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24749	.	engineered_region	3804	5900	.	+	0	ID=BBa_J24712;Name=BBa_J24712;Parent=BBa_J24724
BBa_J24749	.	engineered_region	3804	4975	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24712
BBa_J24749	.	CDS	3823	4950	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24749	.	engineered_region	3823	4950	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24749	.	engineered_tag	4951	4975	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24749	.	CDS	3823	4950	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24749	.	tag	4912	4950	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24749	.	ribosome_entry_site	3804	3816	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24749	.	engineered_region	3804	3816	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24749	.	ribosome_entry_site	3804	3816	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24749	.	ribosome_entry_site	3810	3813	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24749	.	engineered_region	4984	5900	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24712
BBa_J24749	.	terminator	5772	5851	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24749	.	stem_loop	5783	5826	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24749	.	engineered_region	5772	5851	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24749	.	ribosome_entry_site	4984	4995	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24749	.	conserved_region	4988	4991	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24749	.	terminator	5860	5900	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24749	.	polyA_site	5887	5900	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24749	.	stop_codon	5893	5893	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24749	.	stem_loop	5867	5886	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24749	.	engineered_region	5860	5900	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24749	.	engineered_region	5002	5763	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24749	.	stop_codon	5758	5763	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24749	.	CDS	5002	5763	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24749	.	engineered_region	5002	5763	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24749	.	tag	5719	5757	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24749	.	sequence_alteration	5243	5243	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24749	.	engineered_region	1	2765	.	+	0	ID=BBa_J24716;Name=BBa_J24716
BBa_J24749	.	engineered_region	1	1168	.	+	0	ID=BBa_J24691;Name=BBa_J24691;Parent=BBa_J24716
BBa_J24749	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24691
BBa_J24749	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24749	.	CDS	229	1168	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24691
BBa_J24749	.	tag	1105	1137	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24749	.	CDS	229	1104	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24749	.	engineered_tag	1144	1168	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24749	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24691
BBa_J24749	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24749	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24749	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24749	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24749	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24749	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24749	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24749	.	engineered_region	1177	2765	.	+	0	ID=BBa_J24707;Name=BBa_J24707;Parent=BBa_J24716
BBa_J24749	.	engineered_region	1888	2765	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24707
BBa_J24749	.	terminator	2725	2765	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24749	.	polyA_site	2752	2765	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24749	.	stop_codon	2758	2758	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24749	.	stem_loop	2732	2751	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24749	.	engineered_region	2725	2765	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24749	.	engineered_region	1906	2628	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24749	.	tag	2614	2622	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24749	.	sequence_alteration	2257	2257	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24749	.	CDS	1906	2613	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24749	.	ribosome_entry_site	1888	1899	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24749	.	conserved_region	1892	1895	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24749	.	terminator	2637	2716	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24749	.	stem_loop	2648	2691	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24749	.	engineered_region	2637	2716	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24749	.	engineered_region	1177	1879	.	+	0	ID=BBa_J24688;Name=BBa_J24688;Parent=BBa_J24707
BBa_J24749	.	CDS	1195	1879	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24688
BBa_J24749	.	CDS	1198	1814	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24749	.	tag	1815	1848	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24749	.	engineered_tag	1855	1879	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24749	.	ribosome_entry_site	1177	1188	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24688
BBa_J24749	.	conserved_region	1181	1184	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J24749
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggctgaa
gcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatg
gttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggt
ggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactac
ggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggctta
actggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgg
gcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactg
cggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcg
atcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatct
tttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagc
actacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaa
aatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggag
aaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggag
aaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatgg
agggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaa
gctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcc
tacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatga
acttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagct
gcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatg
taccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgagg
tcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctc
ccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctg
tacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacagg
aaacctactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggttt
aacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctc
accattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgc
caggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgc
ctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccg
catttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatc
tgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcga
ggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcg
ccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagcc
aggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactcta
tttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgat
gtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagat
cactactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccg
gtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagc
ggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgtt
gccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgcca
gcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgt
cagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcg
ttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtgg
agcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtct
ggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtcc
ggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgc
tgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccga
agacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgc
ttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccc
tggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctcta
ctagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagc
tggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccct
gaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgc
ttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgca
ccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcat
cgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaac
gtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcg
tgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgag
ctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggg
atcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata