##gff-version 3
##sequence-region BBa_J24753 1 5899
BBa_J24753	.	engineered_region	2775	5899	.	+	0	ID=BBa_J24728;Name=BBa_J24728
BBa_J24753	.	engineered_region	3804	5899	.	+	0	ID=BBa_J24713;Name=BBa_J24713;Parent=BBa_J24728
BBa_J24753	.	engineered_region	3804	4974	.	+	0	ID=BBa_J24685;Name=BBa_J24685;Parent=BBa_J24713
BBa_J24753	.	ribosome_entry_site	3804	3815	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24685
BBa_J24753	.	conserved_region	3808	3811	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24753	.	CDS	3822	4949	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24685
BBa_J24753	.	engineered_region	3822	4949	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24753	.	tag	4911	4949	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24753	.	engineered_tag	4950	4974	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24753	.	CDS	3822	4949	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24753	.	engineered_region	4983	5899	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24713
BBa_J24753	.	terminator	5771	5850	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24753	.	engineered_region	5771	5850	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24753	.	stem_loop	5782	5825	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24753	.	terminator	5859	5899	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24753	.	polyA_site	5886	5899	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24753	.	stop_codon	5892	5892	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24753	.	engineered_region	5859	5899	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24753	.	stem_loop	5866	5885	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24753	.	engineered_region	5001	5762	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24753	.	stop_codon	5757	5762	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24753	.	tag	5718	5756	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24753	.	engineered_region	5001	5762	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24753	.	CDS	5001	5762	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24753	.	sequence_alteration	5242	5242	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24753	.	ribosome_entry_site	4983	4994	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24753	.	conserved_region	4987	4990	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24753	.	engineered_region	2775	3795	.	+	0	ID=BBa_J24695;Name=BBa_J24695;Parent=BBa_J24728
BBa_J24753	.	promoter	2775	2828	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24695
BBa_J24753	.	non_covalent_binding_site	2800	2818	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24753	.	promoter	2817	2822	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24753	.	promoter	2794	2799	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24753	.	engineered_region	2775	2828	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24753	.	non_covalent_binding_site	2775	2793	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24753	.	ribosome_entry_site	2837	2849	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24695
BBa_J24753	.	engineered_region	2837	2849	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24753	.	ribosome_entry_site	2837	2849	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24753	.	ribosome_entry_site	2843	2846	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24753	.	CDS	2856	3795	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24695
BBa_J24753	.	engineered_tag	3771	3795	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24753	.	CDS	2856	3731	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24753	.	tag	3732	3764	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24753	.	engineered_region	1	2766	.	+	0	ID=BBa_J24717;Name=BBa_J24717
BBa_J24753	.	engineered_region	1	1167	.	+	0	ID=BBa_J24692;Name=BBa_J24692;Parent=BBa_J24717
BBa_J24753	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24692
BBa_J24753	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24753	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24753	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24753	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24753	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24753	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24753	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24753	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24692
BBa_J24753	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24753	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24753	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24753	.	CDS	228	1167	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24692
BBa_J24753	.	engineered_tag	1143	1167	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24753	.	CDS	228	1103	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24753	.	tag	1104	1136	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24753	.	engineered_region	1176	2766	.	+	0	ID=BBa_J24705;Name=BBa_J24705;Parent=BBa_J24717
BBa_J24753	.	engineered_region	1889	2766	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24705
BBa_J24753	.	terminator	2726	2766	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24753	.	polyA_site	2753	2766	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24753	.	stop_codon	2759	2759	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24753	.	engineered_region	2726	2766	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24753	.	stem_loop	2733	2752	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24753	.	engineered_region	1907	2629	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24753	.	tag	2615	2623	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24753	.	CDS	1907	2614	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24753	.	sequence_alteration	2258	2258	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24753	.	ribosome_entry_site	1889	1900	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24753	.	conserved_region	1893	1896	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24753	.	terminator	2638	2717	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24753	.	engineered_region	2638	2717	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24753	.	stem_loop	2649	2692	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24753	.	engineered_region	1176	1880	.	+	0	ID=BBa_J24686;Name=BBa_J24686;Parent=BBa_J24705
BBa_J24753	.	CDS	1196	1880	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24753	.	tag	1816	1849	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24753	.	engineered_tag	1856	1880	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24753	.	CDS	1199	1815	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24753	.	ribosome_entry_site	1176	1189	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24753	.	conserved_region	1182	1185	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_J24753
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaag
cgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatgg
ttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtg
gtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacg
gtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaa
ctggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttggg
caaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgc
ggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcga
tcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatctt
ttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagca
ctacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaa
atgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagga
aacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaa
ggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcggg
ctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggca
agattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggt
acacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagaga
atgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa
agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggt
gcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccg
ctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagga
gaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatg
gagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcca
agctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggc
ctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatg
aacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagc
tgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggat
gtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgag
gtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacct
cccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagct
gtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacag
gaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggttt
aacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctc
accattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgc
caggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgc
ctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccg
catttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatc
tgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcga
ggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcg
ccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagcc
aggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactcta
tttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgat
gtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagat
cactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccgg
tgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcg
gcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttg
ccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccag
cgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtc
agtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgt
tatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtgga
gcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctg
gctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccg
gttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgct
gggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaa
gacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgct
tgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccct
ggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactg
gaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctac
tagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagct
ggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctg
aagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgct
tcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcac
catcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatc
gagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacg
tctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgt
gcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagc
taccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggga
tcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata