LOCUS BBa_J24756 5899 bp DNA linear 26-Nov-2024 DEFINITION 76. 39(BBa_J24719)==46(BBa_J24726) ACCESSION BBa_J24756 VERSION BBa_J24756.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 misc_feature 1180..1183 /label=conserved RBS 1176..1187 /label=BBa_B0034 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1176..1878 /label=BBa_J24688 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1176..2764 /label=BBa_J24707 misc_feature 1..2764 /label=BBa_J24719 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 RBS 2835..2847 /label=RBS-3\Weak RBS 2841..2844 /label=RBS misc_feature 2835..2847 /label=BBa_B0032 RBS 2835..2847 /label=BBa_B0032 CDS 2854..3729 /label=araC misc_feature 3730..3762 /label=LVA misc_feature 3769..3793 /label=Help:Barcodes CDS 2854..3793 /label=BBa_C0080 misc_feature 2773..3793 /label=BBa_J24695 misc_feature 3808..3811 /label=conserved RBS 3802..3815 /label=BBa_B0031 misc_feature 4911..4949 /label=LVA CDS 3822..4949 /label=lacI-LVA misc_feature 3822..4949 /label=BBa_C0012 misc_feature 4950..4974 /label=Help:Barcodes CDS 3822..4949 /label=BBa_C0012 misc_feature 3802..4974 /label=BBa_J24683 misc_feature 4987..4990 /label=conserved RBS 4983..4994 /label=BBa_B0034 CDS 5001..5762 /label=YFP (LVA) misc_feature 5757..5762 /label=2 misc_feature 5718..5756 /label=SsrA misc_feature 5242..5242 /label=A (Q->L) misc_feature 5001..5762 /label=BBa_E0032 misc_feature 5001..5762 /label=BBa_E0032 stem_loop 5782..5825 /label=stem_loop misc_feature 5771..5850 /label=BBa_B0010 terminator 5771..5850 /label=BBa_B0010 stem_loop 5866..5885 /label=T7 TE misc_feature 5892..5892 /label=stop polyA_site 5886..5899 /label=polya misc_feature 5859..5899 /label=BBa_B0012 terminator 5859..5899 /label=BBa_B0012 misc_feature 4983..5899 /label=BBa_J24704 misc_feature 3802..5899 /label=BBa_J24711 misc_feature 2773..5899 /label=BBa_J24726 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagtcacac aggaaagtac tagatggctg aagcgcaaaa tgatcccctg 2881 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat 2941 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc 3001 accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt 3061 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc 3121 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac 3181 tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat 3241 ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag 3301 ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag 3361 tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac 3421 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg 3481 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac 3541 caacgcatca gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc 3601 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc 3661 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc 3721 aagttgtcag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 3781 tagtgtagat cactactaga gtcacacagg aaacctacta gatggtgaat gtgaaaccag 3841 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3901 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3961 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 4021 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 4081 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 4141 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 4201 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 4261 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 4321 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 4381 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4441 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4501 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4561 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4621 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4681 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4741 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4801 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4861 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4921 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4981 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 5041 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5101 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5161 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc 5221 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg 5281 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5341 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5401 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 5461 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 5521 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5581 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 5641 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 5701 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 5761 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 5821 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 5881 gggcctttct gcgtttata //