##gff-version 3
##sequence-region BBa_J24757 1 5898
BBa_J24757	.	engineered_region	1	2764	.	+	0	ID=BBa_J24719;Name=BBa_J24719
BBa_J24757	.	engineered_region	1176	2764	.	+	0	ID=BBa_J24707;Name=BBa_J24707;Parent=BBa_J24719
BBa_J24757	.	engineered_region	1176	1878	.	+	0	ID=BBa_J24688;Name=BBa_J24688;Parent=BBa_J24707
BBa_J24757	.	CDS	1194	1878	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24688
BBa_J24757	.	engineered_tag	1854	1878	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24757	.	CDS	1197	1813	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24757	.	tag	1814	1847	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24757	.	ribosome_entry_site	1176	1187	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24688
BBa_J24757	.	conserved_region	1180	1183	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24757	.	engineered_region	1887	2764	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24707
BBa_J24757	.	terminator	2724	2764	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24757	.	engineered_region	2724	2764	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24757	.	stem_loop	2731	2750	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24757	.	stop_codon	2757	2757	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24757	.	polyA_site	2751	2764	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24757	.	ribosome_entry_site	1887	1898	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24757	.	conserved_region	1891	1894	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24757	.	engineered_region	1905	2627	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24757	.	tag	2613	2621	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24757	.	CDS	1905	2612	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24757	.	sequence_alteration	2256	2256	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24757	.	terminator	2636	2715	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24757	.	engineered_region	2636	2715	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24757	.	stem_loop	2647	2690	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24757	.	engineered_region	1	1167	.	+	0	ID=BBa_J24692;Name=BBa_J24692;Parent=BBa_J24719
BBa_J24757	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24692
BBa_J24757	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24757	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24757	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24757	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24757	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24757	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24757	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24757	.	CDS	228	1167	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24692
BBa_J24757	.	CDS	228	1103	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24757	.	engineered_tag	1143	1167	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24757	.	tag	1104	1136	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24757	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24692
BBa_J24757	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24757	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24757	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24757	.	engineered_region	2773	5898	.	+	0	ID=BBa_J24727;Name=BBa_J24727
BBa_J24757	.	engineered_region	3802	5898	.	+	0	ID=BBa_J24712;Name=BBa_J24712;Parent=BBa_J24727
BBa_J24757	.	engineered_region	4982	5898	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24712
BBa_J24757	.	terminator	5770	5849	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24757	.	engineered_region	5770	5849	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24757	.	stem_loop	5781	5824	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24757	.	ribosome_entry_site	4982	4993	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24757	.	conserved_region	4986	4989	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24757	.	terminator	5858	5898	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24757	.	engineered_region	5858	5898	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24757	.	stem_loop	5865	5884	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24757	.	stop_codon	5891	5891	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24757	.	polyA_site	5885	5898	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24757	.	engineered_region	5000	5761	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24757	.	CDS	5000	5761	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24757	.	sequence_alteration	5241	5241	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24757	.	engineered_region	5000	5761	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24757	.	stop_codon	5756	5761	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24757	.	tag	5717	5755	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24757	.	engineered_region	3802	4973	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24712
BBa_J24757	.	ribosome_entry_site	3802	3814	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24757	.	ribosome_entry_site	3802	3814	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24757	.	ribosome_entry_site	3808	3811	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24757	.	engineered_region	3802	3814	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24757	.	CDS	3821	4948	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24757	.	engineered_tag	4949	4973	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24757	.	CDS	3821	4948	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24757	.	tag	4910	4948	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24757	.	engineered_region	3821	4948	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24757	.	engineered_region	2773	3793	.	+	0	ID=BBa_J24695;Name=BBa_J24695;Parent=BBa_J24727
BBa_J24757	.	CDS	2854	3793	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24695
BBa_J24757	.	CDS	2854	3729	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24757	.	engineered_tag	3769	3793	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24757	.	tag	3730	3762	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24757	.	ribosome_entry_site	2835	2847	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24695
BBa_J24757	.	ribosome_entry_site	2835	2847	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24757	.	ribosome_entry_site	2841	2844	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24757	.	engineered_region	2835	2847	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24757	.	promoter	2773	2826	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24695
BBa_J24757	.	promoter	2815	2820	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24757	.	promoter	2792	2797	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24757	.	engineered_region	2773	2826	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24757	.	non_covalent_binding_site	2798	2816	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24757	.	non_covalent_binding_site	2773	2791	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_J24757
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaag
cgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatgg
ttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtg
gtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacg
gtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaa
ctggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttggg
caaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgc
ggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcga
tcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatctt
ttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagca
ctacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaa
atgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggaga
aatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaagg
tttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggct
ttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaag
attttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtac
acggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggaga
aatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatgga
gggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaag
ctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcct
acgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaa
cttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctg
cgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgt
accccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggt
caagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcc
cacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgt
acaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacagga
aagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaa
cgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcac
cattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgcca
ggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcct
actggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgca
tttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctg
cttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgagg
cttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgcc
gtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccag
gcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatt
tctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgt
agccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatca
ctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggt
gtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcgg
cgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgc
cacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagc
gtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtca
gtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtt
atttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggag
catctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctgg
ctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccgg
ttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctg
ggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaag
acagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgctt
gctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctg
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgg
aaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctg
gacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctga
agttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgctt
cgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcg
agctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgt
ctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtg
cagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagct
accagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggat
cactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata