##gff-version 3
##sequence-region BBa_J24760 1 5898
BBa_J24760	.	engineered_region	2774	5898	.	+	0	ID=BBa_J24730;Name=BBa_J24730
BBa_J24760	.	engineered_region	2774	3793	.	+	0	ID=BBa_J24696;Name=BBa_J24696;Parent=BBa_J24730
BBa_J24760	.	engineered_region	2836	3793	.	+	0	ID=BBa_J24682;Name=BBa_J24682;Parent=BBa_J24696
BBa_J24760	.	ribosome_entry_site	2836	2847	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24682
BBa_J24760	.	conserved_region	2840	2843	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24760	.	CDS	2854	3793	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24682
BBa_J24760	.	engineered_tag	3769	3793	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24760	.	tag	3730	3762	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24760	.	CDS	2854	3729	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24760	.	promoter	2774	2827	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24696
BBa_J24760	.	promoter	2793	2798	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24760	.	promoter	2816	2821	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24760	.	non_covalent_binding_site	2774	2792	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24760	.	engineered_region	2774	2827	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24760	.	non_covalent_binding_site	2799	2817	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24760	.	engineered_region	3802	5898	.	+	0	ID=BBa_J24712;Name=BBa_J24712;Parent=BBa_J24730
BBa_J24760	.	engineered_region	3802	4973	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24712
BBa_J24760	.	CDS	3821	4948	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24760	.	engineered_region	3821	4948	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24760	.	CDS	3821	4948	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24760	.	engineered_tag	4949	4973	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24760	.	tag	4910	4948	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24760	.	ribosome_entry_site	3802	3814	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24760	.	engineered_region	3802	3814	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24760	.	ribosome_entry_site	3802	3814	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24760	.	ribosome_entry_site	3808	3811	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24760	.	engineered_region	4982	5898	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24712
BBa_J24760	.	terminator	5770	5849	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24760	.	stem_loop	5781	5824	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24760	.	engineered_region	5770	5849	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24760	.	terminator	5858	5898	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24760	.	polyA_site	5885	5898	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24760	.	stem_loop	5865	5884	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24760	.	engineered_region	5858	5898	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24760	.	stop_codon	5891	5891	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24760	.	engineered_region	5000	5761	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24760	.	stop_codon	5756	5761	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24760	.	sequence_alteration	5241	5241	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24760	.	engineered_region	5000	5761	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24760	.	CDS	5000	5761	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24760	.	tag	5717	5755	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24760	.	ribosome_entry_site	4982	4993	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24760	.	conserved_region	4986	4989	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24760	.	engineered_region	1	2765	.	+	0	ID=BBa_J24720;Name=BBa_J24720
BBa_J24760	.	engineered_region	1	1166	.	+	0	ID=BBa_J24693;Name=BBa_J24693;Parent=BBa_J24720
BBa_J24760	.	CDS	227	1166	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24693
BBa_J24760	.	engineered_tag	1142	1166	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24760	.	tag	1103	1135	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24760	.	CDS	227	1102	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24760	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24693
BBa_J24760	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24760	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24760	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24760	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24760	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24760	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24760	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24760	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24693
BBa_J24760	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24760	.	engineered_region	1175	2765	.	+	0	ID=BBa_J24705;Name=BBa_J24705;Parent=BBa_J24720
BBa_J24760	.	engineered_region	1175	1879	.	+	0	ID=BBa_J24686;Name=BBa_J24686;Parent=BBa_J24705
BBa_J24760	.	ribosome_entry_site	1175	1188	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J24686
BBa_J24760	.	conserved_region	1181	1184	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J24760	.	CDS	1195	1879	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24686
BBa_J24760	.	CDS	1198	1814	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24760	.	tag	1815	1848	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24760	.	engineered_tag	1855	1879	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24760	.	engineered_region	1888	2765	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24705
BBa_J24760	.	terminator	2637	2716	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24760	.	stem_loop	2648	2691	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24760	.	engineered_region	2637	2716	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24760	.	ribosome_entry_site	1888	1899	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24760	.	conserved_region	1892	1895	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24760	.	terminator	2725	2765	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24760	.	polyA_site	2752	2765	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24760	.	stem_loop	2732	2751	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24760	.	engineered_region	2725	2765	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24760	.	stop_codon	2758	2758	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24760	.	engineered_region	1906	2628	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24760	.	tag	2614	2622	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24760	.	CDS	1906	2613	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24760	.	sequence_alteration	2257	2257	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
>BBa_J24760
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgaagc
gcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggt
tatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtgg
tgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacgg
tcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaac
tggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggc
aaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcg
gcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgat
cacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttt
tccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcac
tacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaa
tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaa
acctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggag
aaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatgg
agggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaa
gctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcc
tacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatga
acttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagct
gcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatg
taccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgagg
tcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctc
ccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctg
tacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaa
cgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcac
cattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgcca
ggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcct
actggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgca
tttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctg
cttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgagg
cttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgcc
gtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccag
gcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatt
tctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgt
agccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatca
ctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggt
gtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcgg
cgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgc
cacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagc
gtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtca
gtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtt
atttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggag
catctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctgg
ctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccgg
ttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctg
ggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaag
acagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgctt
gctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctg
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgg
aaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctg
gacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctga
agttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgctt
cgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacc
atcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcg
agctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgt
ctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtg
cagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagct
accagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggat
cactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata