LOCUS BBa_J24761 5897 bp DNA linear 23-Nov-2024 DEFINITION 81. 40(BBa_J24720)==51(BBa_J24731) ACCESSION BBa_J24761 VERSION BBa_J24761.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1181..1184 /label=conserved RBS 1175..1188 /label=BBa_B0031 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1175..1879 /label=BBa_J24686 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1175..2765 /label=BBa_J24705 misc_feature 1..2765 /label=BBa_J24720 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 misc_feature 2840..2843 /label=conserved RBS 2836..2847 /label=BBa_B0034 CDS 2854..3729 /label=araC misc_feature 3730..3762 /label=LVA misc_feature 3769..3793 /label=Help:Barcodes CDS 2854..3793 /label=BBa_C0080 misc_feature 2836..3793 /label=BBa_J24682 misc_feature 2774..3793 /label=BBa_J24696 misc_feature 3806..3809 /label=conserved RBS 3802..3813 /label=BBa_B0034 misc_feature 4909..4947 /label=LVA CDS 3820..4947 /label=lacI-LVA misc_feature 3820..4947 /label=BBa_C0012 misc_feature 4948..4972 /label=Help:Barcodes CDS 3820..4947 /label=BBa_C0012 misc_feature 3802..4972 /label=BBa_J24685 misc_feature 4985..4988 /label=conserved RBS 4981..4992 /label=BBa_B0034 CDS 4999..5760 /label=YFP (LVA) misc_feature 5755..5760 /label=2 misc_feature 5716..5754 /label=SsrA misc_feature 5240..5240 /label=A (Q->L) misc_feature 4999..5760 /label=BBa_E0032 misc_feature 4999..5760 /label=BBa_E0032 stem_loop 5780..5823 /label=stem_loop misc_feature 5769..5848 /label=BBa_B0010 terminator 5769..5848 /label=BBa_B0010 stem_loop 5864..5883 /label=T7 TE misc_feature 5890..5890 /label=stop polyA_site 5884..5897 /label=polya misc_feature 5857..5897 /label=BBa_B0012 terminator 5857..5897 /label=BBa_B0012 misc_feature 4981..5897 /label=BBa_J24704 misc_feature 3802..5897 /label=BBa_J24713 misc_feature 2774..5897 /label=BBa_J24731 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaaccta ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagaaaga ggagaaatac tagatggctg aagcgcaaaa tgatcccctg 2881 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat 2941 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc 3001 accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt 3061 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc 3121 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac 3181 tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat 3241 ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag 3301 ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag 3361 tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac 3421 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg 3481 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac 3541 caacgcatca gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc 3601 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc 3661 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc 3721 aagttgtcag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 3781 tagtgtagat cactactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta 3841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 3901 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3961 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 4021 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 4081 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 4141 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 4201 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 4261 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 4321 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 4381 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 4441 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 4501 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 4561 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 4621 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 4681 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 4741 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 4801 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 4921 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4981 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 5041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 5101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 5161 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 5221 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 5281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 5341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 5401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 5461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5581 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 5641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5701 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 5761 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 5821 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 5881 gcctttctgc gtttata //