LOCUS BBa_J24762 5898 bp DNA linear 27-Dec-2024 DEFINITION 82. 41(BBa_J24721)==49(BBa_J24729) ACCESSION BBa_J24762 VERSION BBa_J24762.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 RBS 1175..1187 /label=RBS-3\Weak RBS 1181..1184 /label=RBS misc_feature 1175..1187 /label=BBa_B0032 RBS 1175..1187 /label=BBa_B0032 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1175..1878 /label=BBa_J24687 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1175..2764 /label=BBa_J24706 misc_feature 1..2764 /label=BBa_J24721 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 misc_feature 2839..2842 /label=conserved RBS 2835..2846 /label=BBa_B0034 CDS 2853..3728 /label=araC misc_feature 3729..3761 /label=LVA misc_feature 3768..3792 /label=Help:Barcodes CDS 2853..3792 /label=BBa_C0080 misc_feature 2835..3792 /label=BBa_J24682 misc_feature 2773..3792 /label=BBa_J24696 misc_feature 3807..3810 /label=conserved RBS 3801..3814 /label=BBa_B0031 misc_feature 4910..4948 /label=LVA CDS 3821..4948 /label=lacI-LVA misc_feature 3821..4948 /label=BBa_C0012 misc_feature 4949..4973 /label=Help:Barcodes CDS 3821..4948 /label=BBa_C0012 misc_feature 3801..4973 /label=BBa_J24683 misc_feature 4986..4989 /label=conserved RBS 4982..4993 /label=BBa_B0034 CDS 5000..5761 /label=YFP (LVA) misc_feature 5756..5761 /label=2 misc_feature 5717..5755 /label=SsrA misc_feature 5241..5241 /label=A (Q->L) misc_feature 5000..5761 /label=BBa_E0032 misc_feature 5000..5761 /label=BBa_E0032 stem_loop 5781..5824 /label=stem_loop misc_feature 5770..5849 /label=BBa_B0010 terminator 5770..5849 /label=BBa_B0010 stem_loop 5865..5884 /label=T7 TE misc_feature 5891..5891 /label=stop polyA_site 5885..5898 /label=polya misc_feature 5858..5898 /label=BBa_B0012 terminator 5858..5898 /label=BBa_B0012 misc_feature 4982..5898 /label=BBa_J24704 misc_feature 3801..5898 /label=BBa_J24711 misc_feature 2773..5898 /label=BBa_J24729 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaagtac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc 2881 tgccgggata ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg 2941 gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca 3001 ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg 3061 atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg 3121 aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact 3181 ggccgtcaat atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt 3241 tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc 3301 tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt 3361 cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc 3421 tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc 3481 gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc 3541 aacgcatcag ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg 3601 gtcgcaatgt tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg 3661 gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca 3721 agttgtcagc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 3781 agtgtagatc actactagag tcacacagga aacctactag atggtgaatg tgaaaccagt 3841 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 3901 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 3961 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 4021 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 4081 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 4141 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 4201 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 4261 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 4321 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 4381 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 4441 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 4501 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 4561 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 4621 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 4681 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 4741 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 4801 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 4861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga 4921 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 4981 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 5041 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 5101 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 5161 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 5221 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 5281 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 5341 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 5401 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 5461 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 5521 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 5581 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 5641 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 5701 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 5761 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 5821 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 5881 ggcctttctg cgtttata //