LOCUS BBa_J24764 5896 bp DNA linear 23-Nov-2024 DEFINITION 84. 41(BBa_J24721)==51(BBa_J24731) ACCESSION BBa_J24764 VERSION BBa_J24764.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 RBS 1175..1187 /label=RBS-3\Weak RBS 1181..1184 /label=RBS misc_feature 1175..1187 /label=BBa_B0032 RBS 1175..1187 /label=BBa_B0032 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1175..1878 /label=BBa_J24687 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1175..2764 /label=BBa_J24706 misc_feature 1..2764 /label=BBa_J24721 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 misc_feature 2839..2842 /label=conserved RBS 2835..2846 /label=BBa_B0034 CDS 2853..3728 /label=araC misc_feature 3729..3761 /label=LVA misc_feature 3768..3792 /label=Help:Barcodes CDS 2853..3792 /label=BBa_C0080 misc_feature 2835..3792 /label=BBa_J24682 misc_feature 2773..3792 /label=BBa_J24696 misc_feature 3805..3808 /label=conserved RBS 3801..3812 /label=BBa_B0034 misc_feature 4908..4946 /label=LVA CDS 3819..4946 /label=lacI-LVA misc_feature 3819..4946 /label=BBa_C0012 misc_feature 4947..4971 /label=Help:Barcodes CDS 3819..4946 /label=BBa_C0012 misc_feature 3801..4971 /label=BBa_J24685 misc_feature 4984..4987 /label=conserved RBS 4980..4991 /label=BBa_B0034 CDS 4998..5759 /label=YFP (LVA) misc_feature 5754..5759 /label=2 misc_feature 5715..5753 /label=SsrA misc_feature 5239..5239 /label=A (Q->L) misc_feature 4998..5759 /label=BBa_E0032 misc_feature 4998..5759 /label=BBa_E0032 stem_loop 5779..5822 /label=stem_loop misc_feature 5768..5847 /label=BBa_B0010 terminator 5768..5847 /label=BBa_B0010 stem_loop 5863..5882 /label=T7 TE misc_feature 5889..5889 /label=stop polyA_site 5883..5896 /label=polya misc_feature 5856..5896 /label=BBa_B0012 terminator 5856..5896 /label=BBa_B0012 misc_feature 4980..5896 /label=BBa_J24704 misc_feature 3801..5896 /label=BBa_J24713 misc_feature 2773..5896 /label=BBa_J24731 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaagtac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc 2881 tgccgggata ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg 2941 gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca 3001 ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg 3061 atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg 3121 aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact 3181 ggccgtcaat atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt 3241 tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc 3301 tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt 3361 cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc 3421 tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc 3481 gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc 3541 aacgcatcag ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg 3601 gtcgcaatgt tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg 3661 gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca 3721 agttgtcagc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 3781 agtgtagatc actactagag aaagaggaga aatactagat ggtgaatgtg aaaccagtaa 3841 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 3901 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 3961 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 4021 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 4081 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 4141 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 4201 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 4261 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 4321 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 4381 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 4441 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 4501 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 4561 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 4621 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 4681 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 4741 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 4801 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg 4921 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 4981 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 5041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 5101 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 5161 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct 5221 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 5281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 5341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 5401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 5461 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 5521 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 5581 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 5641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 5701 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat 5761 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5821 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5881 cctttctgcg tttata //