LOCUS BBa_J24765 5897 bp DNA linear 23-Nov-2024 DEFINITION 85. 42(BBa_J24722)==49(BBa_J24729) ACCESSION BBa_J24765 VERSION BBa_J24765.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1179..1182 /label=conserved RBS 1175..1186 /label=BBa_B0034 CDS 1196..1812 /label=tetR misc_feature 1813..1846 /label=SsrA misc_feature 1853..1877 /label=Help:Barcodes CDS 1193..1877 /label=BBa_C0040 misc_feature 1175..1877 /label=BBa_J24688 misc_feature 1890..1893 /label=conserved RBS 1886..1897 /label=BBa_B0034 CDS 1904..2611 /label=mCherry misc_feature 2255..2255 /label=C->T (removing PstI site) misc_feature 2612..2620 /label=LVA misc_feature 1904..2626 /label=BBa_J06505 stem_loop 2646..2689 /label=stem_loop misc_feature 2635..2714 /label=BBa_B0010 terminator 2635..2714 /label=BBa_B0010 stem_loop 2730..2749 /label=T7 TE misc_feature 2756..2756 /label=stop polyA_site 2750..2763 /label=polya misc_feature 2723..2763 /label=BBa_B0012 terminator 2723..2763 /label=BBa_B0012 misc_feature 1886..2763 /label=BBa_J24702 misc_feature 1175..2763 /label=BBa_J24707 misc_feature 1..2763 /label=BBa_J24722 misc_feature 2772..2790 /label=TetR 1 misc_feature 2772..2825 /label=BBa_R0040 promoter 2791..2796 /label=-35 misc_feature 2797..2815 /label=TetR 2 promoter 2814..2819 /label=-10 promoter 2772..2825 /label=BBa_R0040 misc_feature 2838..2841 /label=conserved RBS 2834..2845 /label=BBa_B0034 CDS 2852..3727 /label=araC misc_feature 3728..3760 /label=LVA misc_feature 3767..3791 /label=Help:Barcodes CDS 2852..3791 /label=BBa_C0080 misc_feature 2834..3791 /label=BBa_J24682 misc_feature 2772..3791 /label=BBa_J24696 misc_feature 3806..3809 /label=conserved RBS 3800..3813 /label=BBa_B0031 misc_feature 4909..4947 /label=LVA CDS 3820..4947 /label=lacI-LVA misc_feature 3820..4947 /label=BBa_C0012 misc_feature 4948..4972 /label=Help:Barcodes CDS 3820..4947 /label=BBa_C0012 misc_feature 3800..4972 /label=BBa_J24683 misc_feature 4985..4988 /label=conserved RBS 4981..4992 /label=BBa_B0034 CDS 4999..5760 /label=YFP (LVA) misc_feature 5755..5760 /label=2 misc_feature 5716..5754 /label=SsrA misc_feature 5240..5240 /label=A (Q->L) misc_feature 4999..5760 /label=BBa_E0032 misc_feature 4999..5760 /label=BBa_E0032 stem_loop 5780..5823 /label=stem_loop misc_feature 5769..5848 /label=BBa_B0010 terminator 5769..5848 /label=BBa_B0010 stem_loop 5864..5883 /label=T7 TE misc_feature 5890..5890 /label=stop polyA_site 5884..5897 /label=polya misc_feature 5857..5897 /label=BBa_B0012 terminator 5857..5897 /label=BBa_B0012 misc_feature 4981..5897 /label=BBa_J24704 misc_feature 3800..5897 /label=BBa_J24711 misc_feature 2772..5897 /label=BBa_J24729 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatgtccag 1201 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga 1261 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg 1321 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca 1381 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc 1441 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac 1501 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca 1561 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg 1621 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac 1681 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga 1741 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg 1801 tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 1861 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga 1921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 1981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2221 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa 2281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 2341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 2401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 2461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 2521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 2581 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc 2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2701 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2761 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 2821 agcactacta gagaaagagg agaaatacta gatggctgaa gcgcaaaatg atcccctgct 2881 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg 2941 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac 3001 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga 3061 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga 3121 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg 3181 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt 3241 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct 3301 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc 3361 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct 3421 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg 3481 tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca 3541 acgcatcagc caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg 3601 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg 3661 ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa 3721 gttgtcagct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 3781 gtgtagatca ctactagagt cacacaggaa acctactaga tggtgaatgt gaaaccagta 3841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 3901 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3961 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 4021 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 4081 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 4141 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 4201 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 4261 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 4321 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 4381 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 4441 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 4501 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 4561 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 4621 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 4681 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 4741 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 4801 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 4921 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4981 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 5041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 5101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 5161 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 5221 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 5281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 5341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 5401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 5461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5581 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 5641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5701 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 5761 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 5821 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 5881 gcctttctgc gtttata //