LOCUS BBa_J24766 5896 bp DNA linear 19-Apr-2025 DEFINITION 86. 42(BBa_J24722)==50(BBa_J24730) ACCESSION BBa_J24766 VERSION BBa_J24766.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1179..1182 /label=conserved RBS 1175..1186 /label=BBa_B0034 CDS 1196..1812 /label=tetR misc_feature 1813..1846 /label=SsrA misc_feature 1853..1877 /label=Help:Barcodes CDS 1193..1877 /label=BBa_C0040 misc_feature 1175..1877 /label=BBa_J24688 misc_feature 1890..1893 /label=conserved RBS 1886..1897 /label=BBa_B0034 CDS 1904..2611 /label=mCherry misc_feature 2255..2255 /label=C->T (removing PstI site) misc_feature 2612..2620 /label=LVA misc_feature 1904..2626 /label=BBa_J06505 stem_loop 2646..2689 /label=stem_loop misc_feature 2635..2714 /label=BBa_B0010 terminator 2635..2714 /label=BBa_B0010 stem_loop 2730..2749 /label=T7 TE misc_feature 2756..2756 /label=stop polyA_site 2750..2763 /label=polya misc_feature 2723..2763 /label=BBa_B0012 terminator 2723..2763 /label=BBa_B0012 misc_feature 1886..2763 /label=BBa_J24702 misc_feature 1175..2763 /label=BBa_J24707 misc_feature 1..2763 /label=BBa_J24722 misc_feature 2772..2790 /label=TetR 1 misc_feature 2772..2825 /label=BBa_R0040 promoter 2791..2796 /label=-35 misc_feature 2797..2815 /label=TetR 2 promoter 2814..2819 /label=-10 promoter 2772..2825 /label=BBa_R0040 misc_feature 2838..2841 /label=conserved RBS 2834..2845 /label=BBa_B0034 CDS 2852..3727 /label=araC misc_feature 3728..3760 /label=LVA misc_feature 3767..3791 /label=Help:Barcodes CDS 2852..3791 /label=BBa_C0080 misc_feature 2834..3791 /label=BBa_J24682 misc_feature 2772..3791 /label=BBa_J24696 RBS 3800..3812 /label=RBS-3\Weak RBS 3806..3809 /label=RBS misc_feature 3800..3812 /label=BBa_B0032 RBS 3800..3812 /label=BBa_B0032 misc_feature 4908..4946 /label=LVA CDS 3819..4946 /label=lacI-LVA misc_feature 3819..4946 /label=BBa_C0012 misc_feature 4947..4971 /label=Help:Barcodes CDS 3819..4946 /label=BBa_C0012 misc_feature 3800..4971 /label=BBa_J24684 misc_feature 4984..4987 /label=conserved RBS 4980..4991 /label=BBa_B0034 CDS 4998..5759 /label=YFP (LVA) misc_feature 5754..5759 /label=2 misc_feature 5715..5753 /label=SsrA misc_feature 5239..5239 /label=A (Q->L) misc_feature 4998..5759 /label=BBa_E0032 misc_feature 4998..5759 /label=BBa_E0032 stem_loop 5779..5822 /label=stem_loop misc_feature 5768..5847 /label=BBa_B0010 terminator 5768..5847 /label=BBa_B0010 stem_loop 5863..5882 /label=T7 TE misc_feature 5889..5889 /label=stop polyA_site 5883..5896 /label=polya misc_feature 5856..5896 /label=BBa_B0012 terminator 5856..5896 /label=BBa_B0012 misc_feature 4980..5896 /label=BBa_J24704 misc_feature 3800..5896 /label=BBa_J24712 misc_feature 2772..5896 /label=BBa_J24730 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatgtccag 1201 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga 1261 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg 1321 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca 1381 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc 1441 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac 1501 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca 1561 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg 1621 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac 1681 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga 1741 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg 1801 tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 1861 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga 1921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 1981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2221 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa 2281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 2341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 2401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 2461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 2521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 2581 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc 2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2701 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2761 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 2821 agcactacta gagaaagagg agaaatacta gatggctgaa gcgcaaaatg atcccctgct 2881 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg 2941 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac 3001 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga 3061 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga 3121 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg 3181 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt 3241 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct 3301 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc 3361 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct 3421 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg 3481 tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca 3541 acgcatcagc caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg 3601 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg 3661 ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa 3721 gttgtcagct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 3781 gtgtagatca ctactagagt cacacaggaa agtactagat ggtgaatgtg aaaccagtaa 3841 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 3901 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 3961 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 4021 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 4081 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 4141 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 4201 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 4261 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 4321 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 4381 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 4441 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 4501 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 4561 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 4621 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 4681 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 4741 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 4801 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg 4921 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 4981 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 5041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 5101 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 5161 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct 5221 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 5281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 5341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 5401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 5461 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 5521 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 5581 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 5641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 5701 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat 5761 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5821 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5881 cctttctgcg tttata //