##gff-version 3
##sequence-region BBa_J24766 1 5896
BBa_J24766	.	engineered_region	1	2763	.	+	0	ID=BBa_J24722;Name=BBa_J24722
BBa_J24766	.	engineered_region	1	1166	.	+	0	ID=BBa_J24693;Name=BBa_J24693;Parent=BBa_J24722
BBa_J24766	.	CDS	227	1166	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24693
BBa_J24766	.	engineered_tag	1142	1166	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24766	.	tag	1103	1135	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24766	.	CDS	227	1102	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24766	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J24693
BBa_J24766	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J24766	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J24766	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J24766	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J24766	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J24766	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J24766	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J24766	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24693
BBa_J24766	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24766	.	engineered_region	1175	2763	.	+	0	ID=BBa_J24707;Name=BBa_J24707;Parent=BBa_J24722
BBa_J24766	.	engineered_region	1175	1877	.	+	0	ID=BBa_J24688;Name=BBa_J24688;Parent=BBa_J24707
BBa_J24766	.	CDS	1193	1877	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24688
BBa_J24766	.	CDS	1196	1812	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24766	.	tag	1813	1846	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24766	.	engineered_tag	1853	1877	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24766	.	ribosome_entry_site	1175	1186	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24688
BBa_J24766	.	conserved_region	1179	1182	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24766	.	engineered_region	1886	2763	.	+	0	ID=BBa_J24702;Name=BBa_J24702;Parent=BBa_J24707
BBa_J24766	.	terminator	2635	2714	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24766	.	stem_loop	2646	2689	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24766	.	engineered_region	2635	2714	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24766	.	ribosome_entry_site	1886	1897	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24766	.	conserved_region	1890	1893	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24766	.	terminator	2723	2763	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24766	.	polyA_site	2750	2763	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24766	.	stem_loop	2730	2749	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24766	.	engineered_region	2723	2763	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24766	.	stop_codon	2756	2756	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24766	.	engineered_region	1904	2626	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24766	.	tag	2612	2620	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24766	.	CDS	1904	2611	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24766	.	sequence_alteration	2255	2255	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24766	.	engineered_region	2772	5896	.	+	0	ID=BBa_J24730;Name=BBa_J24730
BBa_J24766	.	engineered_region	2772	3791	.	+	0	ID=BBa_J24696;Name=BBa_J24696;Parent=BBa_J24730
BBa_J24766	.	engineered_region	2834	3791	.	+	0	ID=BBa_J24682;Name=BBa_J24682;Parent=BBa_J24696
BBa_J24766	.	ribosome_entry_site	2834	2845	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24682
BBa_J24766	.	conserved_region	2838	2841	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24766	.	CDS	2852	3791	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_J24682
BBa_J24766	.	engineered_tag	3767	3791	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J24766	.	tag	3728	3760	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J24766	.	CDS	2852	3727	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J24766	.	promoter	2772	2825	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J24696
BBa_J24766	.	promoter	2791	2796	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J24766	.	promoter	2814	2819	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J24766	.	non_covalent_binding_site	2772	2790	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J24766	.	engineered_region	2772	2825	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J24766	.	non_covalent_binding_site	2797	2815	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J24766	.	engineered_region	3800	5896	.	+	0	ID=BBa_J24712;Name=BBa_J24712;Parent=BBa_J24730
BBa_J24766	.	engineered_region	3800	4971	.	+	0	ID=BBa_J24684;Name=BBa_J24684;Parent=BBa_J24712
BBa_J24766	.	CDS	3819	4946	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_J24684
BBa_J24766	.	engineered_region	3819	4946	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J24766	.	CDS	3819	4946	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J24766	.	engineered_tag	4947	4971	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J24766	.	tag	4908	4946	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J24766	.	ribosome_entry_site	3800	3812	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24684
BBa_J24766	.	engineered_region	3800	3812	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24766	.	ribosome_entry_site	3800	3812	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24766	.	ribosome_entry_site	3806	3809	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24766	.	engineered_region	4980	5896	.	+	0	ID=BBa_J24704;Name=BBa_J24704;Parent=BBa_J24712
BBa_J24766	.	terminator	5768	5847	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24704
BBa_J24766	.	stem_loop	5779	5822	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24766	.	engineered_region	5768	5847	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24766	.	terminator	5856	5896	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24704
BBa_J24766	.	polyA_site	5883	5896	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24766	.	stem_loop	5863	5882	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J24766	.	engineered_region	5856	5896	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24766	.	stop_codon	5889	5889	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24766	.	engineered_region	4998	5759	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_J24704
BBa_J24766	.	stop_codon	5754	5759	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J24766	.	sequence_alteration	5239	5239	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J24766	.	engineered_region	4998	5759	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J24766	.	CDS	4998	5759	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J24766	.	tag	5715	5753	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J24766	.	ribosome_entry_site	4980	4991	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24704
BBa_J24766	.	conserved_region	4984	4987	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J24766
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgaagc
gcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggt
tatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtgg
tgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacgg
tcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaac
tggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggc
aaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcg
gcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgat
cacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttt
tccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcac
tacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaa
tgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaa
atactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggt
ttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctt
tgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaaga
ttttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtaca
cggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatg
cattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaaga
agaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgca
gagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaa
atactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggag
ggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagc
tgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggccta
cgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaac
ttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgc
gcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgta
ccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtc
aagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctccc
acaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgta
caagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaa
atactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacg
ccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcacca
ttcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccagg
agagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctac
tggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatt
tcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgct
tgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggct
tgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgt
cgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggc
gaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttc
tcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtag
ccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact
actagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgt
ctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcg
atggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgcca
cctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgt
ggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagt
gggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttat
ttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagca
tctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggct
ggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggtt
ttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctggg
cgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagac
agctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgc
tgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggc
gcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaa
agcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactag
agaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgga
cggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaag
ttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcg
cccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccat
cttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgag
ctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtct
atatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgca
gctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctac
cagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatca
ctctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata