LOCUS BBa_J24767 5895 bp DNA linear 26-Nov-2024 DEFINITION 87. 42(BBa_J24722)==51(BBa_J24731) ACCESSION BBa_J24767 VERSION BBa_J24767.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1179..1182 /label=conserved RBS 1175..1186 /label=BBa_B0034 CDS 1196..1812 /label=tetR misc_feature 1813..1846 /label=SsrA misc_feature 1853..1877 /label=Help:Barcodes CDS 1193..1877 /label=BBa_C0040 misc_feature 1175..1877 /label=BBa_J24688 misc_feature 1890..1893 /label=conserved RBS 1886..1897 /label=BBa_B0034 CDS 1904..2611 /label=mCherry misc_feature 2255..2255 /label=C->T (removing PstI site) misc_feature 2612..2620 /label=LVA misc_feature 1904..2626 /label=BBa_J06505 stem_loop 2646..2689 /label=stem_loop misc_feature 2635..2714 /label=BBa_B0010 terminator 2635..2714 /label=BBa_B0010 stem_loop 2730..2749 /label=T7 TE misc_feature 2756..2756 /label=stop polyA_site 2750..2763 /label=polya misc_feature 2723..2763 /label=BBa_B0012 terminator 2723..2763 /label=BBa_B0012 misc_feature 1886..2763 /label=BBa_J24702 misc_feature 1175..2763 /label=BBa_J24707 misc_feature 1..2763 /label=BBa_J24722 misc_feature 2772..2790 /label=TetR 1 misc_feature 2772..2825 /label=BBa_R0040 promoter 2791..2796 /label=-35 misc_feature 2797..2815 /label=TetR 2 promoter 2814..2819 /label=-10 promoter 2772..2825 /label=BBa_R0040 misc_feature 2838..2841 /label=conserved RBS 2834..2845 /label=BBa_B0034 CDS 2852..3727 /label=araC misc_feature 3728..3760 /label=LVA misc_feature 3767..3791 /label=Help:Barcodes CDS 2852..3791 /label=BBa_C0080 misc_feature 2834..3791 /label=BBa_J24682 misc_feature 2772..3791 /label=BBa_J24696 misc_feature 3804..3807 /label=conserved RBS 3800..3811 /label=BBa_B0034 misc_feature 4907..4945 /label=LVA CDS 3818..4945 /label=lacI-LVA misc_feature 3818..4945 /label=BBa_C0012 misc_feature 4946..4970 /label=Help:Barcodes CDS 3818..4945 /label=BBa_C0012 misc_feature 3800..4970 /label=BBa_J24685 misc_feature 4983..4986 /label=conserved RBS 4979..4990 /label=BBa_B0034 CDS 4997..5758 /label=YFP (LVA) misc_feature 5753..5758 /label=2 misc_feature 5714..5752 /label=SsrA misc_feature 5238..5238 /label=A (Q->L) misc_feature 4997..5758 /label=BBa_E0032 misc_feature 4997..5758 /label=BBa_E0032 stem_loop 5778..5821 /label=stem_loop misc_feature 5767..5846 /label=BBa_B0010 terminator 5767..5846 /label=BBa_B0010 stem_loop 5862..5881 /label=T7 TE misc_feature 5888..5888 /label=stop polyA_site 5882..5895 /label=polya misc_feature 5855..5895 /label=BBa_B0012 terminator 5855..5895 /label=BBa_B0012 misc_feature 4979..5895 /label=BBa_J24704 misc_feature 3800..5895 /label=BBa_J24713 misc_feature 2772..5895 /label=BBa_J24731 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatgtccag 1201 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga 1261 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg 1321 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca 1381 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc 1441 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac 1501 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca 1561 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg 1621 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac 1681 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga 1741 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg 1801 tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 1861 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga 1921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 1981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2221 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa 2281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 2341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 2401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 2461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 2521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 2581 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc 2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2701 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2761 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 2821 agcactacta gagaaagagg agaaatacta gatggctgaa gcgcaaaatg atcccctgct 2881 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg 2941 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac 3001 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga 3061 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga 3121 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg 3181 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt 3241 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct 3301 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc 3361 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct 3421 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg 3481 tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca 3541 acgcatcagc caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg 3601 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg 3661 ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa 3721 gttgtcagct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 3781 gtgtagatca ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac 3841 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 3901 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 3961 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 4021 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 4081 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 4141 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 4201 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 4261 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 4321 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 4381 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 4441 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 4501 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 4561 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 4621 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 4681 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 4741 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 4801 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 4861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga 4921 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa 4981 agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 5041 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 5101 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 5161 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta 5221 ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca 5281 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 5341 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 5401 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 5461 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 5521 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 5581 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa 5641 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 5701 cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata 5761 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 5821 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 5881 ctttctgcgt ttata //