BBa_J24767
type: ComponentDefinition
persistent identity: BBa J24767
display id: BBa_J24767
version: 1
was generated by: iGEM to SBOL conversion
description: 87. 42(BBa_J24722)==51(BBa_J24731)
created: 2007-04-09T11:00:00Z
modified: 2015-08-31T04:08:43Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: pLacI=S RBS=AraC=S RBS=TetR=S RBS=mCherry (+LVA)=terminator=pTetR=S RBS=AraC=S RBS=LacI=S RBS= EYFP(+LVA)=terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1357
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Norris Hung
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24731
component: BBa_J24722
sequence annotation: BBa_J24722
sequence annotation: BBa_J24731
sequence: https://synbiohub.org/public/igem/BBa_J24767_sequence/1

BBa_J24731
type: Component
persistent identity: component2317690
display id: component2317690
version: 1
top level: BBa_J24767
definition: BBa_J24731
access: public

BBa_J24722
type: Component
persistent identity: component2317654
display id: component2317654
version: 1
top level: BBa_J24767
definition: BBa_J24722
access: public

BBa_J24722
type: SequenceAnnotation
persistent identity: annotation2317654
display id: annotation2317654
version: 1
top level: BBa_J24767
location: https://synbiohub.org/public/igem/BBa_J24767/annotation2317654/range2317654/1
component: BBa_J24722

https://synbiohub.org/public/igem/BBa_J24767/annotation2317654/range2317654/1
type: Range
persistent identity: range2317654
display id: range2317654
version: 1
top level: BBa_J24767
direction: direction/reverse
start: 1
end: 2763
orientation: inline

BBa_J24731
type: SequenceAnnotation
persistent identity: annotation2317690
display id: annotation2317690
version: 1
top level: BBa_J24767
location: https://synbiohub.org/public/igem/BBa_J24767/annotation2317690/range2317690/1
component: BBa_J24731

https://synbiohub.org/public/igem/BBa_J24767/annotation2317690/range2317690/1
type: Range
persistent identity: range2317690
display id: range2317690
version: 1
top level: BBa_J24767
direction: direction/reverse
start: 2772
end: 5895
orientation: inline

BBa_J24722
type: ComponentDefinition
persistent identity: BBa J24722
display id: BBa_J24722
version: 1
was generated by: iGEM to SBOL conversion
description: 42. 15(BBa_J24693)==27(BBa_J24707)
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 42. 15(BBa_J24693)==27(BBa_J24707)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24707
component: BBa_J24693
sequence annotation: BBa_J24693
sequence annotation: BBa_J24707
sequence: https://synbiohub.org/public/igem/BBa_J24722_sequence/1

BBa_J24707
type: Component
persistent identity: component2275050
display id: component2275050
version: 1
top level: BBa_J24722
definition: BBa_J24707
access: public

BBa_J24693
type: Component
persistent identity: component2275029
display id: component2275029
version: 1
top level: BBa_J24722
definition: BBa_J24693
access: public

BBa_J24693
type: SequenceAnnotation
persistent identity: annotation2275029
display id: annotation2275029
version: 1
top level: BBa_J24722
location: https://synbiohub.org/public/igem/BBa_J24722/annotation2275029/range2275029/1
component: BBa_J24693

https://synbiohub.org/public/igem/BBa_J24722/annotation2275029/range2275029/1
type: Range
persistent identity: range2275029
display id: range2275029
version: 1
top level: BBa_J24722
direction: direction/reverse
start: 1
end: 1166
orientation: inline

BBa_J24707
type: SequenceAnnotation
persistent identity: annotation2275050
display id: annotation2275050
version: 1
top level: BBa_J24722
location: https://synbiohub.org/public/igem/BBa_J24722/annotation2275050/range2275050/1
component: BBa_J24707

https://synbiohub.org/public/igem/BBa_J24722/annotation2275050/range2275050/1
type: Range
persistent identity: range2275050
display id: range2275050
version: 1
top level: BBa_J24722
direction: direction/reverse
start: 1175
end: 2763
orientation: inline

BBa_J24731
type: ComponentDefinition
persistent identity: BBa J24731
display id: BBa_J24731
version: 1
was generated by: iGEM to SBOL conversion
description: 51. 18(BBa_J24696)==33(BBa_J247130)
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 51. 18(BBa_J24696)==33(BBa_J247130)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24696
component: BBa_J24713
sequence annotation: BBa_J24696
sequence annotation: BBa_J24713
sequence: https://synbiohub.org/public/igem/BBa_J24731_sequence/1

BBa_J24696
type: Component
persistent identity: component2275343
display id: component2275343
version: 1
top level: BBa_J24731
definition: BBa_J24696
access: public

BBa_J24713
type: Component
persistent identity: component2275365
display id: component2275365
version: 1
top level: BBa_J24731
definition: BBa_J24713
access: public

BBa_J24696
type: SequenceAnnotation
persistent identity: annotation2275343
display id: annotation2275343
version: 1
top level: BBa_J24731
location: https://synbiohub.org/public/igem/BBa_J24731/annotation2275343/range2275343/1
component: BBa_J24696

https://synbiohub.org/public/igem/BBa_J24731/annotation2275343/range2275343/1
type: Range
persistent identity: range2275343
display id: range2275343
version: 1
top level: BBa_J24731
direction: direction/reverse
start: 1
end: 1020
orientation: inline

BBa_J24713
type: SequenceAnnotation
persistent identity: annotation2275365
display id: annotation2275365
version: 1
top level: BBa_J24731
location: https://synbiohub.org/public/igem/BBa_J24731/annotation2275365/range2275365/1
component: BBa_J24713

https://synbiohub.org/public/igem/BBa_J24731/annotation2275365/range2275365/1
type: Range
persistent identity: range2275365
display id: range2275365
version: 1
top level: BBa_J24731
direction: direction/reverse
start: 1029
end: 3124
orientation: inline

BBa_J24693
type: ComponentDefinition
persistent identity: BBa J24693
display id: BBa_J24693
version: 1
was generated by: iGEM to SBOL conversion
description: 15. pLacI - BBa_J24689
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: This is pLacI with S RBS and AraC
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1357
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Norris Hung
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0010
component: BBa_C0080
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_J24693_sequence/1

BBa_R0010
type: Component
persistent identity: component2245459
display id: component2245459
version: 1
top level: BBa_J24693
definition: LacI
access: public

BBa_C0080
type: Component
persistent identity: component2245471
display id: component2245471
version: 1
top level: BBa_J24693
definition: araC
access: public

BBa_B0034
type: Component
persistent identity: component2245467
display id: component2245467
version: 1
top level: BBa_J24693
definition: BBa_B0034
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245459
display id: annotation2245459
version: 1
top level: BBa_J24693
location: https://synbiohub.org/public/igem/BBa_J24693/annotation2245459/range2245459/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J24693/annotation2245459/range2245459/1
type: Range
persistent identity: range2245459
display id: range2245459
version: 1
top level: BBa_J24693
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245467
display id: annotation2245467
version: 1
top level: BBa_J24693
location: https://synbiohub.org/public/igem/BBa_J24693/annotation2245467/range2245467/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24693/annotation2245467/range2245467/1
type: Range
persistent identity: range2245467
display id: range2245467
version: 1
top level: BBa_J24693
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2245471
display id: annotation2245471
version: 1
top level: BBa_J24693
location: https://synbiohub.org/public/igem/BBa_J24693/annotation2245471/range2245471/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_J24693/annotation2245471/range2245471/1
type: Range
persistent identity: range2245471
display id: range2245471
version: 1
top level: BBa_J24693
direction: direction/reverse
start: 227
end: 1166
orientation: inline

BBa_J24696
type: ComponentDefinition
persistent identity: BBa J24696
display id: BBa_J24696
version: 1
was generated by: iGEM to SBOL conversion
description: 18. pTetR - BBa_C0080
created: 2007-04-04T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic
owned by: james
owned by: myers
mutable description: This is pTetR to S RBS and AraC
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1453
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Part only
star: false
creator: Tito Jankowski
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24682
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J24682
sequence: https://synbiohub.org/public/igem/BBa_J24696_sequence/1

BBa_J24682
type: Component
persistent identity: component2245506
display id: component2245506
version: 1
top level: BBa_J24696
definition: BBa_J24682
access: public

BBa_R0040
type: Component
persistent identity: component2245495
display id: component2245495
version: 1
top level: BBa_J24696
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2245495
display id: annotation2245495
version: 1
top level: BBa_J24696
location: https://synbiohub.org/public/igem/BBa_J24696/annotation2245495/range2245495/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J24696/annotation2245495/range2245495/1
type: Range
persistent identity: range2245495
display id: range2245495
version: 1
top level: BBa_J24696
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J24682
type: SequenceAnnotation
persistent identity: annotation2245506
display id: annotation2245506
version: 1
top level: BBa_J24696
location: https://synbiohub.org/public/igem/BBa_J24696/annotation2245506/range2245506/1
component: BBa_J24682

https://synbiohub.org/public/igem/BBa_J24696/annotation2245506/range2245506/1
type: Range
persistent identity: range2245506
display id: range2245506
version: 1
top level: BBa_J24696
direction: direction/reverse
start: 63
end: 1020
orientation: inline

BBa_J24707
type: ComponentDefinition
persistent identity: BBa J24707
display id: BBa_J24707
version: 1
was generated by: iGEM to SBOL conversion
description: 27. S RBS - TetR - S RBS - mCherry (+LVA) - terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: Strong RBS - TetR - Strong RBS - mCherry (+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24702
component: BBa_J24688
sequence annotation: BBa_J24688
sequence annotation: BBa_J24702
sequence: https://synbiohub.org/public/igem/BBa_J24707_sequence/1

BBa_J24702
type: Component
persistent identity: component2260551
display id: component2260551
version: 1
top level: BBa_J24707
definition: BBa_J24702
access: public

BBa_J24688
type: Component
persistent identity: component2260538
display id: component2260538
version: 1
top level: BBa_J24707
definition: BBa_J24688
access: public

BBa_J24688
type: SequenceAnnotation
persistent identity: annotation2260538
display id: annotation2260538
version: 1
top level: BBa_J24707
location: https://synbiohub.org/public/igem/BBa_J24707/annotation2260538/range2260538/1
component: BBa_J24688

https://synbiohub.org/public/igem/BBa_J24707/annotation2260538/range2260538/1
type: Range
persistent identity: range2260538
display id: range2260538
version: 1
top level: BBa_J24707
direction: direction/reverse
start: 1
end: 703
orientation: inline

BBa_J24702
type: SequenceAnnotation
persistent identity: annotation2260551
display id: annotation2260551
version: 1
top level: BBa_J24707
location: https://synbiohub.org/public/igem/BBa_J24707/annotation2260551/range2260551/1
component: BBa_J24702

https://synbiohub.org/public/igem/BBa_J24707/annotation2260551/range2260551/1
type: Range
persistent identity: range2260551
display id: range2260551
version: 1
top level: BBa_J24707
direction: direction/reverse
start: 712
end: 1589
orientation: inline

BBa_J24713
type: ComponentDefinition
persistent identity: BBa J24713
display id: BBa_J24713
version: 1
was generated by: iGEM to SBOL conversion
description: 33. S RBS - LacI - S RBS - EYFP(+LVA) - terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 33. S RBS - LacI - S RBS - EYFP(+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24685
component: BBa_J24704
sequence annotation: BBa_J24685
sequence annotation: BBa_J24704
sequence: https://synbiohub.org/public/igem/BBa_J24713_sequence/1

BBa_J24685
type: Component
persistent identity: component2260665
display id: component2260665
version: 1
top level: BBa_J24713
definition: BBa_J24685
access: public

BBa_J24704
type: Component
persistent identity: component2260679
display id: component2260679
version: 1
top level: BBa_J24713
definition: BBa_J24704
access: public

BBa_J24685
type: SequenceAnnotation
persistent identity: annotation2260665
display id: annotation2260665
version: 1
top level: BBa_J24713
location: https://synbiohub.org/public/igem/BBa_J24713/annotation2260665/range2260665/1
component: BBa_J24685

https://synbiohub.org/public/igem/BBa_J24713/annotation2260665/range2260665/1
type: Range
persistent identity: range2260665
display id: range2260665
version: 1
top level: BBa_J24713
direction: direction/reverse
start: 1
end: 1171
orientation: inline

BBa_J24704
type: SequenceAnnotation
persistent identity: annotation2260679
display id: annotation2260679
version: 1
top level: BBa_J24713
location: https://synbiohub.org/public/igem/BBa_J24713/annotation2260679/range2260679/1
component: BBa_J24704

https://synbiohub.org/public/igem/BBa_J24713/annotation2260679/range2260679/1
type: Range
persistent identity: range2260679
display id: range2260679
version: 1
top level: BBa_J24713
direction: direction/reverse
start: 1180
end: 2096
orientation: inline

BBa_J24682
type: ComponentDefinition
persistent identity: BBa J24682
display id: BBa_J24682
version: 1
was generated by: iGEM to SBOL conversion
description: 3. S RBS - AraC
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: yes
owned by: james
owned by: myers
mutable description: Strong RBS with Arac
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Azeem Kaka
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0080
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_J24682_sequence/1

BBa_C0080
type: Component
persistent identity: component2243844
display id: component2243844
version: 1
top level: BBa_J24682
definition: araC
access: public

BBa_B0034
type: Component
persistent identity: component2243840
display id: component2243840
version: 1
top level: BBa_J24682
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243840
display id: annotation2243840
version: 1
top level: BBa_J24682
location: https://synbiohub.org/public/igem/BBa_J24682/annotation2243840/range2243840/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24682/annotation2243840/range2243840/1
type: Range
persistent identity: range2243840
display id: range2243840
version: 1
top level: BBa_J24682
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2243844
display id: annotation2243844
version: 1
top level: BBa_J24682
location: https://synbiohub.org/public/igem/BBa_J24682/annotation2243844/range2243844/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_J24682/annotation2243844/range2243844/1
type: Range
persistent identity: range2243844
display id: range2243844
version: 1
top level: BBa_J24682
direction: direction/reverse
start: 19
end: 958
orientation: inline

BBa_J24685
type: ComponentDefinition
persistent identity: BBa J24685
display id: BBa_J24685
version: 1
was generated by: iGEM to SBOL conversion
description: 6. Strong RBS + LacI
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: yes
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a strong RBS with the LacI coding region
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no
star: false
creator: Annie Gao
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_J24685_sequence/1

BBa_B0034
type: Component
persistent identity: component2243859
display id: component2243859
version: 1
top level: BBa_J24685
definition: BBa_B0034
access: public

BBa_C0012
type: Component
persistent identity: component2243861
display id: component2243861
version: 1
top level: BBa_J24685
definition: lacI
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243859
display id: annotation2243859
version: 1
top level: BBa_J24685
location: https://synbiohub.org/public/igem/BBa_J24685/annotation2243859/range2243859/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24685/annotation2243859/range2243859/1
type: Range
persistent identity: range2243859
display id: range2243859
version: 1
top level: BBa_J24685
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243861
display id: annotation2243861
version: 1
top level: BBa_J24685
location: https://synbiohub.org/public/igem/BBa_J24685/annotation2243861/range2243861/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J24685/annotation2243861/range2243861/1
type: Range
persistent identity: range2243861
display id: range2243861
version: 1
top level: BBa_J24685
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_J24688
type: ComponentDefinition
persistent identity: BBa J24688
display id: BBa_J24688
version: 1
was generated by: iGEM to SBOL conversion
description: 9. S RBS - TetR
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS with TetR protein coding region
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No
star: false
creator: Annie Gao
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_J24688_sequence/1

BBa_C0040
type: Component
persistent identity: component2243882
display id: component2243882
version: 1
top level: BBa_J24688
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2243878
display id: component2243878
version: 1
top level: BBa_J24688
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243878
display id: annotation2243878
version: 1
top level: BBa_J24688
location: https://synbiohub.org/public/igem/BBa_J24688/annotation2243878/range2243878/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24688/annotation2243878/range2243878/1
type: Range
persistent identity: range2243878
display id: range2243878
version: 1
top level: BBa_J24688
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2243882
display id: annotation2243882
version: 1
top level: BBa_J24688
location: https://synbiohub.org/public/igem/BBa_J24688/annotation2243882/range2243882/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_J24688/annotation2243882/range2243882/1
type: Range
persistent identity: range2243882
display id: range2243882
version: 1
top level: BBa_J24688
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_J24702
type: ComponentDefinition
persistent identity: BBa J24702
display id: BBa_J24702
version: 1
was generated by: iGEM to SBOL conversion
description: 22. BBa_J24700 to terminator
created: 2007-04-07T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic.
owned by: james
owned by: myers
mutable description: S RBS to mCherry reporter to terminator composite.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 792
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jason Lohmueller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_J06505
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J06505
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J24702_sequence/1

BBa_B0012
type: Component
persistent identity: component1923910
display id: component1923910
version: 1
top level: BBa_J24702
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1923908
display id: component1923908
version: 1
top level: BBa_J24702
definition: BBa_B0010
access: public

BBa_J06505
type: Component
persistent identity: component1923907
display id: component1923907
version: 1
top level: BBa_J24702
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1923903
display id: component1923903
version: 1
top level: BBa_J24702
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923903
display id: annotation1923903
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923903/range1923903/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24702/annotation1923903/range1923903/1
type: Range
persistent identity: range1923903
display id: range1923903
version: 1
top level: BBa_J24702
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J06505
type: SequenceAnnotation
persistent identity: annotation1923907
display id: annotation1923907
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923907/range1923907/1
component: BBa_J06505

https://synbiohub.org/public/igem/BBa_J24702/annotation1923907/range1923907/1
type: Range
persistent identity: range1923907
display id: range1923907
version: 1
top level: BBa_J24702
direction: direction/reverse
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923908
display id: annotation1923908
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923908/range1923908/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J24702/annotation1923908/range1923908/1
type: Range
persistent identity: range1923908
display id: range1923908
version: 1
top level: BBa_J24702
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923910
display id: annotation1923910
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923910/range1923910/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J24702/annotation1923910/range1923910/1
type: Range
persistent identity: range1923910
display id: range1923910
version: 1
top level: BBa_J24702
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_J24704
type: ComponentDefinition
persistent identity: BBa J24704
display id: BBa_J24704
version: 1
was generated by: iGEM to SBOL conversion
description: 24. BBa_J24690 to terminator
created: 2007-04-07T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: S-RBS - EYFP to terminator composite.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 792
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none.
star: false
creator: Jason Lohmueller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0032
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J24704_sequence/1

BBa_E0032
type: Component
persistent identity: component1923930
display id: component1923930
version: 1
top level: BBa_J24704
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component1923928
display id: component1923928
version: 1
top level: BBa_J24704
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1923936
display id: component1923936
version: 1
top level: BBa_J24704
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1923934
display id: component1923934
version: 1
top level: BBa_J24704
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923928
display id: annotation1923928
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923928/range1923928/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24704/annotation1923928/range1923928/1
type: Range
persistent identity: range1923928
display id: range1923928
version: 1
top level: BBa_J24704
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1923930
display id: annotation1923930
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923930/range1923930/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J24704/annotation1923930/range1923930/1
type: Range
persistent identity: range1923930
display id: range1923930
version: 1
top level: BBa_J24704
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923934
display id: annotation1923934
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923934/range1923934/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J24704/annotation1923934/range1923934/1
type: Range
persistent identity: range1923934
display id: range1923934
version: 1
top level: BBa_J24704
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923936
display id: annotation1923936
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923936/range1923936/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J24704/annotation1923936/range1923936/1
type: Range
persistent identity: range1923936
display id: range1923936
version: 1
top level: BBa_J24704
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: false
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
top level: mCherry
direction: direction/forward
start: 709
end: 717
orientation: inline

https://synbiohub.org/public/igem/BBa_J24767_sequence/1
type: Sequence
persistent identity: BBa J24767 sequence
display id: BBa_J24767_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24722_sequence/1
type: Sequence
persistent identity: BBa J24722 sequence
display id: BBa_J24722_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24731_sequence/1
type: Sequence
persistent identity: BBa J24731 sequence
display id: BBa_J24731_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24693_sequence/1
type: Sequence
persistent identity: BBa J24693 sequence
display id: BBa_J24693_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24696_sequence/1
type: Sequence
persistent identity: BBa J24696 sequence
display id: BBa_J24696_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24707_sequence/1
type: Sequence
persistent identity: BBa J24707 sequence
display id: BBa_J24707_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24713_sequence/1
type: Sequence
persistent identity: BBa J24713 sequence
display id: BBa_J24713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24682_sequence/1
type: Sequence
persistent identity: BBa J24682 sequence
display id: BBa_J24682_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24685_sequence/1
type: Sequence
persistent identity: BBa J24685 sequence
display id: BBa_J24685_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24688_sequence/1
type: Sequence
persistent identity: BBa J24688 sequence
display id: BBa_J24688_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24702_sequence/1
type: Sequence
persistent identity: BBa J24702 sequence
display id: BBa_J24702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24704_sequence/1
type: Sequence
persistent identity: BBa J24704 sequence
display id: BBa_J24704_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06505_sequence/1
type: Sequence
persistent identity: BBa J06505 sequence
display id: BBa_J06505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z