LOCUS BBa_J24775 9934 bp DNA linear 23-Nov-2024 DEFINITION 93. 68( BBa_J24749) == 57(BBa_J24737) ACCESSION BBa_J24775 VERSION BBa_J24775.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 misc_feature 1181..1184 /label=conserved RBS 1177..1188 /label=BBa_B0034 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1177..1879 /label=BBa_J24688 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1177..2765 /label=BBa_J24707 misc_feature 1..2765 /label=BBa_J24716 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 misc_feature 2842..2845 /label=conserved RBS 2836..2849 /label=BBa_B0031 CDS 2856..3731 /label=araC misc_feature 3732..3764 /label=LVA misc_feature 3771..3795 /label=Help:Barcodes CDS 2856..3795 /label=BBa_C0080 misc_feature 2774..3795 /label=BBa_J24694 RBS 3804..3816 /label=RBS-3\Weak RBS 3810..3813 /label=RBS misc_feature 3804..3816 /label=BBa_B0032 RBS 3804..3816 /label=BBa_B0032 misc_feature 4912..4950 /label=LVA CDS 3823..4950 /label=lacI-LVA misc_feature 3823..4950 /label=BBa_C0012 misc_feature 4951..4975 /label=Help:Barcodes CDS 3823..4950 /label=BBa_C0012 misc_feature 3804..4975 /label=BBa_J24684 misc_feature 4988..4991 /label=conserved RBS 4984..4995 /label=BBa_B0034 CDS 5002..5763 /label=YFP (LVA) misc_feature 5758..5763 /label=2 misc_feature 5719..5757 /label=SsrA misc_feature 5243..5243 /label=A (Q->L) misc_feature 5002..5763 /label=BBa_E0032 misc_feature 5002..5763 /label=BBa_E0032 stem_loop 5783..5826 /label=stem_loop misc_feature 5772..5851 /label=BBa_B0010 terminator 5772..5851 /label=BBa_B0010 stem_loop 5867..5886 /label=T7 TE misc_feature 5893..5893 /label=stop polyA_site 5887..5900 /label=polya misc_feature 5860..5900 /label=BBa_B0012 terminator 5860..5900 /label=BBa_B0012 misc_feature 4984..5900 /label=BBa_J24704 misc_feature 3804..5900 /label=BBa_J24712 misc_feature 2774..5900 /label=BBa_J24724 misc_feature 1..5900 /label=BBa_J24749 promoter 6988..7118 /label=Pbad promoter 6951..7022 /label=PC misc_feature 5909..6987 /label=AraC promoter 5909..7118 /label=BBa_I0500 RBS 7127..7139 /label=RBS-3\Weak RBS 7133..7136 /label=RBS misc_feature 7127..7139 /label=BBa_B0032 RBS 7127..7139 /label=BBa_B0032 misc_feature 8235..8273 /label=LVA CDS 7146..8273 /label=lacI-LVA misc_feature 7146..8273 /label=BBa_C0012 misc_feature 8274..8298 /label=Help:Barcodes CDS 7146..8273 /label=BBa_C0012 misc_feature 7127..8298 /label=BBa_J24684 misc_feature 5909..8298 /label=BBa_J24698 misc_feature 8311..8314 /label=conserved RBS 8307..8318 /label=BBa_B0034 CDS 8328..8944 /label=tetR misc_feature 8945..8978 /label=SsrA misc_feature 8985..9009 /label=Help:Barcodes CDS 8325..9009 /label=BBa_C0040 misc_feature 8307..9009 /label=BBa_J24688 misc_feature 9022..9025 /label=conserved RBS 9018..9029 /label=BBa_B0034 CDS 9036..9797 /label=CFP (LVA) misc_feature 9754..9791 /label=SsrA misc_feature 9792..9797 /label=2 misc_feature 9104..9104 /label=A misc_feature 9036..9797 /label=BBa_E0022 misc_feature 9036..9797 /label=BBa_E0022 stem_loop 9817..9860 /label=stem_loop misc_feature 9806..9885 /label=BBa_B0010 terminator 9806..9885 /label=BBa_B0010 stem_loop 9901..9920 /label=T7 TE misc_feature 9927..9927 /label=stop polyA_site 9921..9934 /label=polya misc_feature 9894..9934 /label=BBa_B0012 terminator 9894..9934 /label=BBa_B0012 misc_feature 9018..9934 /label=BBa_J24703 misc_feature 8307..9934 /label=BBa_J24710 misc_feature 5909..9934 /label=BBa_J24737 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagtcaca caggaaacct actagatggc tgaagcgcaa aatgatcccc 2881 tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca 2941 atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc 3001 tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg 3061 gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc 3121 gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta 3181 actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc 3241 atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg 3301 agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg 3361 agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc 3421 acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt 3481 cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg 3541 accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg 3601 tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca 3661 ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg 3721 tcaagttgtc agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 3781 gctagtgtag atcactacta gagtcacaca ggaaagtact agatggtgaa tgtgaaacca 3841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 3961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 4021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 4081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 4141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 4201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 4261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 4321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 4381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 4441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 4501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 4621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 4681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 4741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 4801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac 4921 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 4981 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 5041 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 5101 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 5161 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 5221 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 5281 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 5341 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 5401 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 5461 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 5521 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 5581 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 5641 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 5701 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 5761 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5821 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5881 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt 5941 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc 6001 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc 6061 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga 6121 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat 6181 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac 6241 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc 6301 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc 6361 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat 6421 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt 6481 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac 6541 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt 6601 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt 6661 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta 6721 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt 6781 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca 6841 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc 6901 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa 6961 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 7021 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 7081 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagtcac acaggaaagt 7141 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 7201 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 7261 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 7321 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 7381 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 7441 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 7501 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 7561 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 7621 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 7681 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 7741 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 7801 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 7861 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 7921 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 7981 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 8041 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 8101 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 8161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 8221 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 8281 agtgctagtg tagatctcta ctagagaaag aggagaaata ctagatgtcc agattagata 8341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 8401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 8461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 8521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 8581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 8641 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 8701 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 8761 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 8821 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 8881 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 8941 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 9001 gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 9061 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 9121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 9181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg 9241 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 9301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 9361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 9421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc 9481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc 9541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 9601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 9661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 9721 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 9781 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 9841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 9901 ggctcacctt cgggtgggcc tttctgcgtt tata //