LOCUS BBa_J24779 9938 bp DNA linear 28-Jun-2024 DEFINITION 97. 70(BBa_J24751) == 52(BBa_J24732) ACCESSION BBa_J24779 VERSION BBa_J24779.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 misc_feature 1182..1185 /label=conserved RBS 1176..1189 /label=BBa_B0031 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1176..1880 /label=BBa_J24686 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1176..2766 /label=BBa_J24705 misc_feature 1..2766 /label=BBa_J24717 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 RBS 2837..2849 /label=RBS-3\Weak RBS 2843..2846 /label=RBS misc_feature 2837..2849 /label=BBa_B0032 RBS 2837..2849 /label=BBa_B0032 CDS 2856..3731 /label=araC misc_feature 3732..3764 /label=LVA misc_feature 3771..3795 /label=Help:Barcodes CDS 2856..3795 /label=BBa_C0080 misc_feature 2775..3795 /label=BBa_J24695 misc_feature 3810..3813 /label=conserved RBS 3804..3817 /label=BBa_B0031 misc_feature 4913..4951 /label=LVA CDS 3824..4951 /label=lacI-LVA misc_feature 3824..4951 /label=BBa_C0012 misc_feature 4952..4976 /label=Help:Barcodes CDS 3824..4951 /label=BBa_C0012 misc_feature 3804..4976 /label=BBa_J24683 misc_feature 4989..4992 /label=conserved RBS 4985..4996 /label=BBa_B0034 CDS 5003..5764 /label=YFP (LVA) misc_feature 5759..5764 /label=2 misc_feature 5720..5758 /label=SsrA misc_feature 5244..5244 /label=A (Q->L) misc_feature 5003..5764 /label=BBa_E0032 misc_feature 5003..5764 /label=BBa_E0032 stem_loop 5784..5827 /label=stem_loop misc_feature 5773..5852 /label=BBa_B0010 terminator 5773..5852 /label=BBa_B0010 stem_loop 5868..5887 /label=T7 TE misc_feature 5894..5894 /label=stop polyA_site 5888..5901 /label=polya misc_feature 5861..5901 /label=BBa_B0012 terminator 5861..5901 /label=BBa_B0012 misc_feature 4985..5901 /label=BBa_J24704 misc_feature 3804..5901 /label=BBa_J24711 misc_feature 2775..5901 /label=BBa_J24726 misc_feature 1..5901 /label=BBa_J24751 promoter 6989..7119 /label=Pbad promoter 6952..7023 /label=PC misc_feature 5910..6988 /label=AraC promoter 5910..7119 /label=BBa_I0500 misc_feature 7134..7137 /label=conserved RBS 7128..7141 /label=BBa_B0031 misc_feature 8237..8275 /label=LVA CDS 7148..8275 /label=lacI-LVA misc_feature 7148..8275 /label=BBa_C0012 misc_feature 8276..8300 /label=Help:Barcodes CDS 7148..8275 /label=BBa_C0012 misc_feature 7128..8300 /label=BBa_J24683 misc_feature 5910..8300 /label=BBa_J24697 misc_feature 8315..8318 /label=conserved RBS 8309..8322 /label=BBa_B0031 CDS 8332..8948 /label=tetR misc_feature 8949..8982 /label=SsrA misc_feature 8989..9013 /label=Help:Barcodes CDS 8329..9013 /label=BBa_C0040 misc_feature 8309..9013 /label=BBa_J24686 misc_feature 9026..9029 /label=conserved RBS 9022..9033 /label=BBa_B0034 CDS 9040..9801 /label=CFP (LVA) misc_feature 9758..9795 /label=SsrA misc_feature 9796..9801 /label=2 misc_feature 9108..9108 /label=A misc_feature 9040..9801 /label=BBa_E0022 misc_feature 9040..9801 /label=BBa_E0022 stem_loop 9821..9864 /label=stem_loop misc_feature 9810..9889 /label=BBa_B0010 terminator 9810..9889 /label=BBa_B0010 stem_loop 9905..9924 /label=T7 TE misc_feature 9931..9931 /label=stop polyA_site 9925..9938 /label=polya misc_feature 9898..9938 /label=BBa_B0012 terminator 9898..9938 /label=BBa_B0012 misc_feature 9022..9938 /label=BBa_J24703 misc_feature 8309..9938 /label=BBa_J24708 misc_feature 5910..9938 /label=BBa_J24732 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaacct actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaagt actagatggc tgaagcgcaa aatgatcccc 2881 tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca 2941 atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc 3001 tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg 3061 gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc 3121 gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta 3181 actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc 3241 atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg 3301 agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg 3361 agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc 3421 acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt 3481 cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg 3541 accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg 3601 tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca 3661 ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg 3721 tcaagttgtc agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 3781 gctagtgtag atcactacta gagtcacaca ggaaacctac tagatggtga atgtgaaacc 3841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 3901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 3961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 4021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 4081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 4141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 4201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 4261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 4321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 4381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 4441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 4501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 4561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 4621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 4681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 4741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 4801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 4861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa 4921 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 4981 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 5041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 5101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 5161 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 5221 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 5281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 5341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 5401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 5461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 5521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 5581 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 5641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 5701 catggacgag ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta 5761 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 5821 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 5881 gtgggccttt ctgcgtttat atactagagt tatgacaact tgacggctac atcattcact 5941 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc 6001 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc 6061 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag 6121 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca 6181 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga 6241 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg 6301 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc 6361 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca 6421 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag 6481 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga 6541 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat 6601 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct 6661 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt 6721 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct 6781 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc 6841 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg 6901 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa 6961 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc 7021 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa 7081 ctctctactg tttctccata cccgtttttt tgggctagct actagagtca cacaggaaac 7141 ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt 7201 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg 7261 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca 7321 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc 7381 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 7441 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 7501 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc 7561 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc 7621 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt 7681 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg 7741 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga 7801 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 7861 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg 7921 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga 7981 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct 8041 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg 8101 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca 8161 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 8221 actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt aataactctg 8281 atagtgctag tgtagatctc tactagagtc acacaggaaa cctactagat gtccagatta 8341 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt 8401 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat 8461 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat 8521 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa 8581 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg 8641 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt 8701 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc 8761 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat 8821 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca 8881 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa 8941 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 9001 tagtgtagat cactactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag 9061 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag 9121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 9181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg 9241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 9301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 9361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 9421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc 9481 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 9541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 9601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 9661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 9721 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac 9781 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 9841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 9901 cactggctca ccttcgggtg ggcctttctg cgtttata //