LOCUS BBa_J24783 9936 bp DNA linear 19-Nov-2024 DEFINITION 100. 71(BBa_J24752) == 55(BBa_J24735) ACCESSION BBa_J24783 VERSION BBa_J24783.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 misc_feature 1182..1185 /label=conserved RBS 1176..1189 /label=BBa_B0031 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1176..1880 /label=BBa_J24686 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1176..2766 /label=BBa_J24705 misc_feature 1..2766 /label=BBa_J24717 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 RBS 2837..2849 /label=RBS-3\Weak RBS 2843..2846 /label=RBS misc_feature 2837..2849 /label=BBa_B0032 RBS 2837..2849 /label=BBa_B0032 CDS 2856..3731 /label=araC misc_feature 3732..3764 /label=LVA misc_feature 3771..3795 /label=Help:Barcodes CDS 2856..3795 /label=BBa_C0080 misc_feature 2775..3795 /label=BBa_J24695 RBS 3804..3816 /label=RBS-3\Weak RBS 3810..3813 /label=RBS misc_feature 3804..3816 /label=BBa_B0032 RBS 3804..3816 /label=BBa_B0032 misc_feature 4912..4950 /label=LVA CDS 3823..4950 /label=lacI-LVA misc_feature 3823..4950 /label=BBa_C0012 misc_feature 4951..4975 /label=Help:Barcodes CDS 3823..4950 /label=BBa_C0012 misc_feature 3804..4975 /label=BBa_J24684 misc_feature 4988..4991 /label=conserved RBS 4984..4995 /label=BBa_B0034 CDS 5002..5763 /label=YFP (LVA) misc_feature 5758..5763 /label=2 misc_feature 5719..5757 /label=SsrA misc_feature 5243..5243 /label=A (Q->L) misc_feature 5002..5763 /label=BBa_E0032 misc_feature 5002..5763 /label=BBa_E0032 stem_loop 5783..5826 /label=stem_loop misc_feature 5772..5851 /label=BBa_B0010 terminator 5772..5851 /label=BBa_B0010 stem_loop 5867..5886 /label=T7 TE misc_feature 5893..5893 /label=stop polyA_site 5887..5900 /label=polya misc_feature 5860..5900 /label=BBa_B0012 terminator 5860..5900 /label=BBa_B0012 misc_feature 4984..5900 /label=BBa_J24704 misc_feature 3804..5900 /label=BBa_J24712 misc_feature 2775..5900 /label=BBa_J24727 misc_feature 1..5900 /label=BBa_J24752 promoter 6988..7118 /label=Pbad promoter 6951..7022 /label=PC misc_feature 5909..6987 /label=AraC promoter 5909..7118 /label=BBa_I0500 RBS 7127..7139 /label=RBS-3\Weak RBS 7133..7136 /label=RBS misc_feature 7127..7139 /label=BBa_B0032 RBS 7127..7139 /label=BBa_B0032 misc_feature 8235..8273 /label=LVA CDS 7146..8273 /label=lacI-LVA misc_feature 7146..8273 /label=BBa_C0012 misc_feature 8274..8298 /label=Help:Barcodes CDS 7146..8273 /label=BBa_C0012 misc_feature 7127..8298 /label=BBa_J24684 misc_feature 5909..8298 /label=BBa_J24698 misc_feature 8313..8316 /label=conserved RBS 8307..8320 /label=BBa_B0031 CDS 8330..8946 /label=tetR misc_feature 8947..8980 /label=SsrA misc_feature 8987..9011 /label=Help:Barcodes CDS 8327..9011 /label=BBa_C0040 misc_feature 8307..9011 /label=BBa_J24686 misc_feature 9024..9027 /label=conserved RBS 9020..9031 /label=BBa_B0034 CDS 9038..9799 /label=CFP (LVA) misc_feature 9756..9793 /label=SsrA misc_feature 9794..9799 /label=2 misc_feature 9106..9106 /label=A misc_feature 9038..9799 /label=BBa_E0022 misc_feature 9038..9799 /label=BBa_E0022 stem_loop 9819..9862 /label=stem_loop misc_feature 9808..9887 /label=BBa_B0010 terminator 9808..9887 /label=BBa_B0010 stem_loop 9903..9922 /label=T7 TE misc_feature 9929..9929 /label=stop polyA_site 9923..9936 /label=polya misc_feature 9896..9936 /label=BBa_B0012 terminator 9896..9936 /label=BBa_B0012 misc_feature 9020..9936 /label=BBa_J24703 misc_feature 8307..9936 /label=BBa_J24708 misc_feature 5909..9936 /label=BBa_J24735 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaacct actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaagt actagatggc tgaagcgcaa aatgatcccc 2881 tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca 2941 atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc 3001 tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg 3061 gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc 3121 gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta 3181 actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc 3241 atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg 3301 agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg 3361 agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc 3421 acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt 3481 cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg 3541 accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg 3601 tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca 3661 ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg 3721 tcaagttgtc agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 3781 gctagtgtag atcactacta gagtcacaca ggaaagtact agatggtgaa tgtgaaacca 3841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 3901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 3961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 4021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 4081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 4141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 4201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 4261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 4321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 4381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 4441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 4501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 4561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 4621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 4681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 4741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 4801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac 4921 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 4981 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 5041 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 5101 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 5161 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 5221 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 5281 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 5341 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 5401 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 5461 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 5521 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 5581 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 5641 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 5701 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 5761 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5821 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5881 tgggcctttc tgcgtttata tactagagtt atgacaactt gacggctaca tcattcactt 5941 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc 6001 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc 6061 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga 6121 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat 6181 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac 6241 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc 6301 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc 6361 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat 6421 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt 6481 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac 6541 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt 6601 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt 6661 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta 6721 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt 6781 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca 6841 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc 6901 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa 6961 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct 7021 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac 7081 tctctactgt ttctccatac ccgttttttt gggctagcta ctagagtcac acaggaaagt 7141 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 7201 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 7261 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 7321 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 7381 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 7441 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 7501 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 7561 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 7621 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 7681 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 7741 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 7801 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 7861 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 7921 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 7981 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 8041 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 8101 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 8161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 8221 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 8281 agtgctagtg tagatctcta ctagagtcac acaggaaacc tactagatgt ccagattaga 8341 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 8401 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 8461 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 8521 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 8581 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 8641 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 8701 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 8761 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 8821 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 8881 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 8941 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 9001 gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 9061 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 9121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 9181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 9241 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 9301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 9361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 9421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 9481 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 9541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 9601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 9661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 9721 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 9781 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 9841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 9901 ctggctcacc ttcgggtggg cctttctgcg tttata //