BBa_J24783
type: ComponentDefinition
persistent identity: BBa J24783
display id: BBa_J24783
version: 1
was generated by: iGEM to SBOL conversion
description: 100. 71(BBa_J24752) == 55(BBa_J24735)
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:08:43Z
mutable provenance: genomic
owned by: james
owned by: myers
mutable description: 100. 71(BBa_J24752) == 55(BBa_J24735)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1453
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Final Projects
star: false
creator: Tito Jankowski
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24752
component: BBa_J24735
sequence annotation: BBa_J24752
sequence annotation: BBa_J24735
sequence: https://synbiohub.org/public/igem/BBa_J24783_sequence/1

BBa_J24752
type: Component
persistent identity: component2325823
display id: component2325823
version: 1
top level: BBa_J24783
definition: BBa_J24752
access: public

BBa_J24735
type: Component
persistent identity: component2325859
display id: component2325859
version: 1
top level: BBa_J24783
definition: BBa_J24735
access: public

BBa_J24752
type: SequenceAnnotation
persistent identity: annotation2325823
display id: annotation2325823
version: 1
top level: BBa_J24783
location: https://synbiohub.org/public/igem/BBa_J24783/annotation2325823/range2325823/1
component: BBa_J24752

https://synbiohub.org/public/igem/BBa_J24783/annotation2325823/range2325823/1
type: Range
persistent identity: range2325823
display id: range2325823
version: 1
top level: BBa_J24783
direction: direction/reverse
start: 1
end: 5900
orientation: inline

BBa_J24735
type: SequenceAnnotation
persistent identity: annotation2325859
display id: annotation2325859
version: 1
top level: BBa_J24783
location: https://synbiohub.org/public/igem/BBa_J24783/annotation2325859/range2325859/1
component: BBa_J24735

https://synbiohub.org/public/igem/BBa_J24783/annotation2325859/range2325859/1
type: Range
persistent identity: range2325859
display id: range2325859
version: 1
top level: BBa_J24783
direction: direction/reverse
start: 5909
end: 9936
orientation: inline

BBa_J24735
type: ComponentDefinition
persistent identity: BBa J24735
display id: BBa_J24735
version: 1
was generated by: iGEM to SBOL conversion
description: 55. 20(BBa_J24698)==28(BBa_J24708)
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 55. 20(BBa_J24698)==28(BBa_J24708)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24708
component: BBa_J24698
sequence annotation: BBa_J24698
sequence annotation: BBa_J24708
sequence: https://synbiohub.org/public/igem/BBa_J24735_sequence/1

BBa_J24708
type: Component
persistent identity: component2275503
display id: component2275503
version: 1
top level: BBa_J24735
definition: BBa_J24708
access: public

BBa_J24698
type: Component
persistent identity: component2275481
display id: component2275481
version: 1
top level: BBa_J24735
definition: BBa_J24698
access: public

BBa_J24698
type: SequenceAnnotation
persistent identity: annotation2275481
display id: annotation2275481
version: 1
top level: BBa_J24735
location: https://synbiohub.org/public/igem/BBa_J24735/annotation2275481/range2275481/1
component: BBa_J24698

https://synbiohub.org/public/igem/BBa_J24735/annotation2275481/range2275481/1
type: Range
persistent identity: range2275481
display id: range2275481
version: 1
top level: BBa_J24735
direction: direction/reverse
start: 1
end: 2390
orientation: inline

BBa_J24708
type: SequenceAnnotation
persistent identity: annotation2275503
display id: annotation2275503
version: 1
top level: BBa_J24735
location: https://synbiohub.org/public/igem/BBa_J24735/annotation2275503/range2275503/1
component: BBa_J24708

https://synbiohub.org/public/igem/BBa_J24735/annotation2275503/range2275503/1
type: Range
persistent identity: range2275503
display id: range2275503
version: 1
top level: BBa_J24735
direction: direction/reverse
start: 2399
end: 4028
orientation: inline

BBa_J24752
type: ComponentDefinition
persistent identity: BBa J24752
display id: BBa_J24752
version: 1
was generated by: iGEM to SBOL conversion
description: 71. 37(BBa_J24717)==47(BBa_J24727)
created: 2007-04-09T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: YES
owned by: james
owned by: myers
mutable description: pLacI=M RBS=AraC=W RBS=TetR=Strong RBS=mCherry(+LVA)=terminator=pTetR=M RBS - AraC=M RBS - LacI - S RBS - EYFP(+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1454
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NONE
star: false
creator: Adam Emrich
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24727
component: BBa_J24717
sequence annotation: BBa_J24717
sequence annotation: BBa_J24727
sequence: https://synbiohub.org/public/igem/BBa_J24752_sequence/1

BBa_J24727
type: Component
persistent identity: component2317326
display id: component2317326
version: 1
top level: BBa_J24752
definition: BBa_J24727
access: public

BBa_J24717
type: Component
persistent identity: component2317289
display id: component2317289
version: 1
top level: BBa_J24752
definition: BBa_J24717
access: public

BBa_J24717
type: SequenceAnnotation
persistent identity: annotation2317289
display id: annotation2317289
version: 1
top level: BBa_J24752
location: https://synbiohub.org/public/igem/BBa_J24752/annotation2317289/range2317289/1
component: BBa_J24717

https://synbiohub.org/public/igem/BBa_J24752/annotation2317289/range2317289/1
type: Range
persistent identity: range2317289
display id: range2317289
version: 1
top level: BBa_J24752
direction: direction/reverse
start: 1
end: 2766
orientation: inline

BBa_J24727
type: SequenceAnnotation
persistent identity: annotation2317326
display id: annotation2317326
version: 1
top level: BBa_J24752
location: https://synbiohub.org/public/igem/BBa_J24752/annotation2317326/range2317326/1
component: BBa_J24727

https://synbiohub.org/public/igem/BBa_J24752/annotation2317326/range2317326/1
type: Range
persistent identity: range2317326
display id: range2317326
version: 1
top level: BBa_J24752
direction: direction/reverse
start: 2775
end: 5900
orientation: inline

BBa_J24698
type: ComponentDefinition
persistent identity: BBa J24698
display id: BBa_J24698
version: 1
was generated by: iGEM to SBOL conversion
description: 20. BBa_I0500 - BBa_J24684
created: 2007-04-04T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic
owned by: james
owned by: myers
mutable description: This is pBAD with 5. M RBS - LacI
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1453
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: part only
star: false
creator: Tito Jankowski
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24684
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_J24684
sequence: https://synbiohub.org/public/igem/BBa_J24698_sequence/1

BBa_J24684
type: Component
persistent identity: component2245529
display id: component2245529
version: 1
top level: BBa_J24698
definition: BBa_J24684
access: public

BBa_I0500
type: Component
persistent identity: component2245521
display id: component2245521
version: 1
top level: BBa_J24698
definition: pBad/araC
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2245521
display id: annotation2245521
version: 1
top level: BBa_J24698
location: https://synbiohub.org/public/igem/BBa_J24698/annotation2245521/range2245521/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J24698/annotation2245521/range2245521/1
type: Range
persistent identity: range2245521
display id: range2245521
version: 1
top level: BBa_J24698
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_J24684
type: SequenceAnnotation
persistent identity: annotation2245529
display id: annotation2245529
version: 1
top level: BBa_J24698
location: https://synbiohub.org/public/igem/BBa_J24698/annotation2245529/range2245529/1
component: BBa_J24684

https://synbiohub.org/public/igem/BBa_J24698/annotation2245529/range2245529/1
type: Range
persistent identity: range2245529
display id: range2245529
version: 1
top level: BBa_J24698
direction: direction/reverse
start: 1219
end: 2390
orientation: inline

BBa_J24708
type: ComponentDefinition
persistent identity: BBa J24708
display id: BBa_J24708
version: 1
was generated by: iGEM to SBOL conversion
description: 28. W RBS - TetR - S RBS - ECFP(+LVA) - terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 28. Weak RBS - TetR - Strong RBS - ECFP(+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24703
component: BBa_J24686
sequence annotation: BBa_J24686
sequence annotation: BBa_J24703
sequence: https://synbiohub.org/public/igem/BBa_J24708_sequence/1

BBa_J24703
type: Component
persistent identity: component2260572
display id: component2260572
version: 1
top level: BBa_J24708
definition: BBa_J24703
access: public

BBa_J24686
type: Component
persistent identity: component2260558
display id: component2260558
version: 1
top level: BBa_J24708
definition: BBa_J24686
access: public

BBa_J24686
type: SequenceAnnotation
persistent identity: annotation2260558
display id: annotation2260558
version: 1
top level: BBa_J24708
location: https://synbiohub.org/public/igem/BBa_J24708/annotation2260558/range2260558/1
component: BBa_J24686

https://synbiohub.org/public/igem/BBa_J24708/annotation2260558/range2260558/1
type: Range
persistent identity: range2260558
display id: range2260558
version: 1
top level: BBa_J24708
direction: direction/reverse
start: 1
end: 705
orientation: inline

BBa_J24703
type: SequenceAnnotation
persistent identity: annotation2260572
display id: annotation2260572
version: 1
top level: BBa_J24708
location: https://synbiohub.org/public/igem/BBa_J24708/annotation2260572/range2260572/1
component: BBa_J24703

https://synbiohub.org/public/igem/BBa_J24708/annotation2260572/range2260572/1
type: Range
persistent identity: range2260572
display id: range2260572
version: 1
top level: BBa_J24708
direction: direction/reverse
start: 714
end: 1630
orientation: inline

BBa_J24717
type: ComponentDefinition
persistent identity: BBa J24717
display id: BBa_J24717
version: 1
was generated by: iGEM to SBOL conversion
description: 37. pLacI=M RBS=AraC=W RBS=TetR=Strong RBS=mCherry(+LVA)=terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 37. pLacI=M RBS=AraC=W RBS=TetR=Strong RBS=mCherry(+LVA)=terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24692
component: BBa_J24705
sequence annotation: BBa_J24692
sequence annotation: BBa_J24705
sequence: https://synbiohub.org/public/igem/BBa_J24717_sequence/1

BBa_J24692
type: Component
persistent identity: component2274850
display id: component2274850
version: 1
top level: BBa_J24717
definition: BBa_J24692
access: public

BBa_J24705
type: Component
persistent identity: component2274871
display id: component2274871
version: 1
top level: BBa_J24717
definition: BBa_J24705
access: public

BBa_J24692
type: SequenceAnnotation
persistent identity: annotation2274850
display id: annotation2274850
version: 1
top level: BBa_J24717
location: https://synbiohub.org/public/igem/BBa_J24717/annotation2274850/range2274850/1
component: BBa_J24692

https://synbiohub.org/public/igem/BBa_J24717/annotation2274850/range2274850/1
type: Range
persistent identity: range2274850
display id: range2274850
version: 1
top level: BBa_J24717
direction: direction/reverse
start: 1
end: 1167
orientation: inline

BBa_J24705
type: SequenceAnnotation
persistent identity: annotation2274871
display id: annotation2274871
version: 1
top level: BBa_J24717
location: https://synbiohub.org/public/igem/BBa_J24717/annotation2274871/range2274871/1
component: BBa_J24705

https://synbiohub.org/public/igem/BBa_J24717/annotation2274871/range2274871/1
type: Range
persistent identity: range2274871
display id: range2274871
version: 1
top level: BBa_J24717
direction: direction/reverse
start: 1176
end: 2766
orientation: inline

BBa_J24727
type: ComponentDefinition
persistent identity: BBa J24727
display id: BBa_J24727
version: 1
was generated by: iGEM to SBOL conversion
description: 47. 17(BBa_J24695)==32(BBa_J24712)
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 47. 17(BBa_J24695)==32(BBa_J24712)
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24712
component: BBa_J24695
sequence annotation: BBa_J24695
sequence annotation: BBa_J24712
sequence: https://synbiohub.org/public/igem/BBa_J24727_sequence/1

BBa_J24712
type: Component
persistent identity: component2275224
display id: component2275224
version: 1
top level: BBa_J24727
definition: BBa_J24712
access: public

BBa_J24695
type: Component
persistent identity: component2275201
display id: component2275201
version: 1
top level: BBa_J24727
definition: BBa_J24695
access: public

BBa_J24695
type: SequenceAnnotation
persistent identity: annotation2275201
display id: annotation2275201
version: 1
top level: BBa_J24727
location: https://synbiohub.org/public/igem/BBa_J24727/annotation2275201/range2275201/1
component: BBa_J24695

https://synbiohub.org/public/igem/BBa_J24727/annotation2275201/range2275201/1
type: Range
persistent identity: range2275201
display id: range2275201
version: 1
top level: BBa_J24727
direction: direction/reverse
start: 1
end: 1021
orientation: inline

BBa_J24712
type: SequenceAnnotation
persistent identity: annotation2275224
display id: annotation2275224
version: 1
top level: BBa_J24727
location: https://synbiohub.org/public/igem/BBa_J24727/annotation2275224/range2275224/1
component: BBa_J24712

https://synbiohub.org/public/igem/BBa_J24727/annotation2275224/range2275224/1
type: Range
persistent identity: range2275224
display id: range2275224
version: 1
top level: BBa_J24727
direction: direction/reverse
start: 1030
end: 3126
orientation: inline

BBa_J24684
type: ComponentDefinition
persistent identity: BBa J24684
display id: BBa_J24684
version: 1
was generated by: iGEM to SBOL conversion
description: 5. M RBS - LacI
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:41Z
mutable provenance: yes
owned by: james
owned by: myers
mutable description: Weak RBS with LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Azeem Kaka
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_J24684_sequence/1

BBa_C0012
type: Component
persistent identity: component2243855
display id: component2243855
version: 1
top level: BBa_J24684
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component2243852
display id: component2243852
version: 1
top level: BBa_J24684
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2243852
display id: annotation2243852
version: 1
top level: BBa_J24684
location: https://synbiohub.org/public/igem/BBa_J24684/annotation2243852/range2243852/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J24684/annotation2243852/range2243852/1
type: Range
persistent identity: range2243852
display id: range2243852
version: 1
top level: BBa_J24684
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243855
display id: annotation2243855
version: 1
top level: BBa_J24684
location: https://synbiohub.org/public/igem/BBa_J24684/annotation2243855/range2243855/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J24684/annotation2243855/range2243855/1
type: Range
persistent identity: range2243855
display id: range2243855
version: 1
top level: BBa_J24684
direction: direction/forward
start: 20
end: 1147
orientation: inline

BBa_J24686
type: ComponentDefinition
persistent identity: BBa J24686
display id: BBa_J24686
version: 1
was generated by: iGEM to SBOL conversion
description: 7. W RBS - TetR
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: A weak RBS with the TetR protein coding region.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Annie Gao
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_J24686_sequence/1

BBa_C0040
type: Component
persistent identity: component2243869
display id: component2243869
version: 1
top level: BBa_J24686
definition: tetR
access: public

BBa_B0031
type: Component
persistent identity: component2243865
display id: component2243865
version: 1
top level: BBa_J24686
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2243865
display id: annotation2243865
version: 1
top level: BBa_J24686
location: https://synbiohub.org/public/igem/BBa_J24686/annotation2243865/range2243865/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J24686/annotation2243865/range2243865/1
type: Range
persistent identity: range2243865
display id: range2243865
version: 1
top level: BBa_J24686
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2243869
display id: annotation2243869
version: 1
top level: BBa_J24686
location: https://synbiohub.org/public/igem/BBa_J24686/annotation2243869/range2243869/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_J24686/annotation2243869/range2243869/1
type: Range
persistent identity: range2243869
display id: range2243869
version: 1
top level: BBa_J24686
direction: direction/reverse
start: 21
end: 705
orientation: inline

BBa_J24692
type: ComponentDefinition
persistent identity: BBa J24692
display id: BBa_J24692
version: 1
was generated by: iGEM to SBOL conversion
description: 14. pLacI - BBa_J24681
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: This is pLacI with M RBS and AraC
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1357
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Norris Hung
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_C0080
component: BBa_B0032
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0032
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_J24692_sequence/1

BBa_C0080
type: Component
persistent identity: component2245458
display id: component2245458
version: 1
top level: BBa_J24692
definition: araC
access: public

BBa_B0032
type: Component
persistent identity: component2245453
display id: component2245453
version: 1
top level: BBa_J24692
definition: BBa_B0032
access: public

BBa_R0010
type: Component
persistent identity: component2245445
display id: component2245445
version: 1
top level: BBa_J24692
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245445
display id: annotation2245445
version: 1
top level: BBa_J24692
location: https://synbiohub.org/public/igem/BBa_J24692/annotation2245445/range2245445/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J24692/annotation2245445/range2245445/1
type: Range
persistent identity: range2245445
display id: range2245445
version: 1
top level: BBa_J24692
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2245453
display id: annotation2245453
version: 1
top level: BBa_J24692
location: https://synbiohub.org/public/igem/BBa_J24692/annotation2245453/range2245453/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J24692/annotation2245453/range2245453/1
type: Range
persistent identity: range2245453
display id: range2245453
version: 1
top level: BBa_J24692
direction: direction/forward
start: 209
end: 221
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2245458
display id: annotation2245458
version: 1
top level: BBa_J24692
location: https://synbiohub.org/public/igem/BBa_J24692/annotation2245458/range2245458/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_J24692/annotation2245458/range2245458/1
type: Range
persistent identity: range2245458
display id: range2245458
version: 1
top level: BBa_J24692
direction: direction/reverse
start: 228
end: 1167
orientation: inline

BBa_J24695
type: ComponentDefinition
persistent identity: BBa J24695
display id: BBa_J24695
version: 1
was generated by: iGEM to SBOL conversion
description: 17. pTetR - BBa_J24681
created: 2007-04-03T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: This is pTetR with M RBS and AraC
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1357
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Norris Hung
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0080
component: BBa_B0032
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_J24695_sequence/1

BBa_C0080
type: Component
persistent identity: component2245494
display id: component2245494
version: 1
top level: BBa_J24695
definition: araC
access: public

BBa_B0032
type: Component
persistent identity: component2245489
display id: component2245489
version: 1
top level: BBa_J24695
definition: BBa_B0032
access: public

BBa_R0040
type: Component
persistent identity: component2245483
display id: component2245483
version: 1
top level: BBa_J24695
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2245483
display id: annotation2245483
version: 1
top level: BBa_J24695
location: https://synbiohub.org/public/igem/BBa_J24695/annotation2245483/range2245483/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J24695/annotation2245483/range2245483/1
type: Range
persistent identity: range2245483
display id: range2245483
version: 1
top level: BBa_J24695
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2245489
display id: annotation2245489
version: 1
top level: BBa_J24695
location: https://synbiohub.org/public/igem/BBa_J24695/annotation2245489/range2245489/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J24695/annotation2245489/range2245489/1
type: Range
persistent identity: range2245489
display id: range2245489
version: 1
top level: BBa_J24695
direction: direction/forward
start: 63
end: 75
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2245494
display id: annotation2245494
version: 1
top level: BBa_J24695
location: https://synbiohub.org/public/igem/BBa_J24695/annotation2245494/range2245494/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_J24695/annotation2245494/range2245494/1
type: Range
persistent identity: range2245494
display id: range2245494
version: 1
top level: BBa_J24695
direction: direction/reverse
start: 82
end: 1021
orientation: inline

BBa_J24703
type: ComponentDefinition
persistent identity: BBa J24703
display id: BBa_J24703
version: 1
was generated by: iGEM to SBOL conversion
description: 23. BBa_24701 to terminator
created: 2007-04-07T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: S-RBS ECFP to terminator composite.
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 792
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none.
star: false
creator: Jason Lohmueller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0022
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J24703_sequence/1

BBa_B0012
type: Component
persistent identity: component1923923
display id: component1923923
version: 1
top level: BBa_J24703
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1923921
display id: component1923921
version: 1
top level: BBa_J24703
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component1923917
display id: component1923917
version: 1
top level: BBa_J24703
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component1923915
display id: component1923915
version: 1
top level: BBa_J24703
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923915
display id: annotation1923915
version: 1
top level: BBa_J24703
location: https://synbiohub.org/public/igem/BBa_J24703/annotation1923915/range1923915/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24703/annotation1923915/range1923915/1
type: Range
persistent identity: range1923915
display id: range1923915
version: 1
top level: BBa_J24703
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1923917
display id: annotation1923917
version: 1
top level: BBa_J24703
location: https://synbiohub.org/public/igem/BBa_J24703/annotation1923917/range1923917/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J24703/annotation1923917/range1923917/1
type: Range
persistent identity: range1923917
display id: range1923917
version: 1
top level: BBa_J24703
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923921
display id: annotation1923921
version: 1
top level: BBa_J24703
location: https://synbiohub.org/public/igem/BBa_J24703/annotation1923921/range1923921/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J24703/annotation1923921/range1923921/1
type: Range
persistent identity: range1923921
display id: range1923921
version: 1
top level: BBa_J24703
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923923
display id: annotation1923923
version: 1
top level: BBa_J24703
location: https://synbiohub.org/public/igem/BBa_J24703/annotation1923923/range1923923/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J24703/annotation1923923/range1923923/1
type: Range
persistent identity: range1923923
display id: range1923923
version: 1
top level: BBa_J24703
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_J24705
type: ComponentDefinition
persistent identity: BBa J24705
display id: BBa_J24705
version: 1
was generated by: iGEM to SBOL conversion
description: 25. Weak RBS - TetR - Strong RBS - mCherry(+LVA) - terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: Weak RBS - TetR - Strong RBS - mCherry(+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J24702
component: BBa_J24686
sequence annotation: BBa_J24686
sequence annotation: BBa_J24702
sequence: https://synbiohub.org/public/igem/BBa_J24705_sequence/1

BBa_J24702
type: Component
persistent identity: component2260510
display id: component2260510
version: 1
top level: BBa_J24705
definition: BBa_J24702
access: public

BBa_J24686
type: Component
persistent identity: component2260497
display id: component2260497
version: 1
top level: BBa_J24705
definition: BBa_J24686
access: public

BBa_J24686
type: SequenceAnnotation
persistent identity: annotation2260497
display id: annotation2260497
version: 1
top level: BBa_J24705
location: https://synbiohub.org/public/igem/BBa_J24705/annotation2260497/range2260497/1
component: BBa_J24686

https://synbiohub.org/public/igem/BBa_J24705/annotation2260497/range2260497/1
type: Range
persistent identity: range2260497
display id: range2260497
version: 1
top level: BBa_J24705
direction: direction/reverse
start: 1
end: 705
orientation: inline

BBa_J24702
type: SequenceAnnotation
persistent identity: annotation2260510
display id: annotation2260510
version: 1
top level: BBa_J24705
location: https://synbiohub.org/public/igem/BBa_J24705/annotation2260510/range2260510/1
component: BBa_J24702

https://synbiohub.org/public/igem/BBa_J24705/annotation2260510/range2260510/1
type: Range
persistent identity: range2260510
display id: range2260510
version: 1
top level: BBa_J24705
direction: direction/reverse
start: 714
end: 1591
orientation: inline

BBa_J24712
type: ComponentDefinition
persistent identity: BBa J24712
display id: BBa_J24712
version: 1
was generated by: iGEM to SBOL conversion
description: 32. M RBS - LacI - S RBS - EYFP(+LVA) - terminator
created: 2007-04-08T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: Yes
owned by: james
owned by: myers
mutable description: 32. M RBS - LacI - S RBS - EYFP(+LVA) - terminator
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 1450
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Deepa Galaiya
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J24704
component: BBa_J24684
sequence annotation: BBa_J24684
sequence annotation: BBa_J24704
sequence: https://synbiohub.org/public/igem/BBa_J24712_sequence/1

BBa_J24704
type: Component
persistent identity: component2260658
display id: component2260658
version: 1
top level: BBa_J24712
definition: BBa_J24704
access: public

BBa_J24684
type: Component
persistent identity: component2260644
display id: component2260644
version: 1
top level: BBa_J24712
definition: BBa_J24684
access: public

BBa_J24684
type: SequenceAnnotation
persistent identity: annotation2260644
display id: annotation2260644
version: 1
top level: BBa_J24712
location: https://synbiohub.org/public/igem/BBa_J24712/annotation2260644/range2260644/1
component: BBa_J24684

https://synbiohub.org/public/igem/BBa_J24712/annotation2260644/range2260644/1
type: Range
persistent identity: range2260644
display id: range2260644
version: 1
top level: BBa_J24712
direction: direction/reverse
start: 1
end: 1172
orientation: inline

BBa_J24704
type: SequenceAnnotation
persistent identity: annotation2260658
display id: annotation2260658
version: 1
top level: BBa_J24712
location: https://synbiohub.org/public/igem/BBa_J24712/annotation2260658/range2260658/1
component: BBa_J24704

https://synbiohub.org/public/igem/BBa_J24712/annotation2260658/range2260658/1
type: Range
persistent identity: range2260658
display id: range2260658
version: 1
top level: BBa_J24712
direction: direction/reverse
start: 1181
end: 2097
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_J24702
type: ComponentDefinition
persistent identity: BBa J24702
display id: BBa_J24702
version: 1
was generated by: iGEM to SBOL conversion
description: 22. BBa_J24700 to terminator
created: 2007-04-07T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic.
owned by: james
owned by: myers
mutable description: S RBS to mCherry reporter to terminator composite.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 792
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none.
star: false
creator: Jason Lohmueller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_J06505
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J06505
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J24702_sequence/1

BBa_B0012
type: Component
persistent identity: component1923910
display id: component1923910
version: 1
top level: BBa_J24702
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1923908
display id: component1923908
version: 1
top level: BBa_J24702
definition: BBa_B0010
access: public

BBa_J06505
type: Component
persistent identity: component1923907
display id: component1923907
version: 1
top level: BBa_J24702
definition: mCherry
access: public

BBa_B0034
type: Component
persistent identity: component1923903
display id: component1923903
version: 1
top level: BBa_J24702
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923903
display id: annotation1923903
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923903/range1923903/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24702/annotation1923903/range1923903/1
type: Range
persistent identity: range1923903
display id: range1923903
version: 1
top level: BBa_J24702
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J06505
type: SequenceAnnotation
persistent identity: annotation1923907
display id: annotation1923907
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923907/range1923907/1
component: BBa_J06505

https://synbiohub.org/public/igem/BBa_J24702/annotation1923907/range1923907/1
type: Range
persistent identity: range1923907
display id: range1923907
version: 1
top level: BBa_J24702
direction: direction/reverse
start: 19
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923908
display id: annotation1923908
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923908/range1923908/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J24702/annotation1923908/range1923908/1
type: Range
persistent identity: range1923908
display id: range1923908
version: 1
top level: BBa_J24702
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923910
display id: annotation1923910
version: 1
top level: BBa_J24702
location: https://synbiohub.org/public/igem/BBa_J24702/annotation1923910/range1923910/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J24702/annotation1923910/range1923910/1
type: Range
persistent identity: range1923910
display id: range1923910
version: 1
top level: BBa_J24702
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_J24704
type: ComponentDefinition
persistent identity: BBa J24704
display id: BBa_J24704
version: 1
was generated by: iGEM to SBOL conversion
description: 24. BBa_J24690 to terminator
created: 2007-04-07T11:00:00Z
modified: 2015-08-31T04:08:42Z
mutable provenance: genomic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: S-RBS - EYFP to terminator composite.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 792
owning group id: 58
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none.
star: false
creator: Jason Lohmueller
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0032
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J24704_sequence/1

BBa_E0032
type: Component
persistent identity: component1923930
display id: component1923930
version: 1
top level: BBa_J24704
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component1923928
display id: component1923928
version: 1
top level: BBa_J24704
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1923936
display id: component1923936
version: 1
top level: BBa_J24704
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1923934
display id: component1923934
version: 1
top level: BBa_J24704
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923928
display id: annotation1923928
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923928/range1923928/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J24704/annotation1923928/range1923928/1
type: Range
persistent identity: range1923928
display id: range1923928
version: 1
top level: BBa_J24704
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1923930
display id: annotation1923930
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923930/range1923930/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J24704/annotation1923930/range1923930/1
type: Range
persistent identity: range1923930
display id: range1923930
version: 1
top level: BBa_J24704
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923934
display id: annotation1923934
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923934/range1923934/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J24704/annotation1923934/range1923934/1
type: Range
persistent identity: range1923934
display id: range1923934
version: 1
top level: BBa_J24704
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923936
display id: annotation1923936
version: 1
top level: BBa_J24704
location: https://synbiohub.org/public/igem/BBa_J24704/annotation1923936/range1923936/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J24704/annotation1923936/range1923936/1
type: Range
persistent identity: range1923936
display id: range1923936
version: 1
top level: BBa_J24704
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: false
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
top level: mCherry
direction: direction/forward
start: 709
end: 717
orientation: inline

https://synbiohub.org/public/igem/BBa_J24783_sequence/1
type: Sequence
persistent identity: BBa J24783 sequence
display id: BBa_J24783_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24735_sequence/1
type: Sequence
persistent identity: BBa J24735 sequence
display id: BBa_J24735_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24752_sequence/1
type: Sequence
persistent identity: BBa J24752 sequence
display id: BBa_J24752_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24698_sequence/1
type: Sequence
persistent identity: BBa J24698 sequence
display id: BBa_J24698_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24708_sequence/1
type: Sequence
persistent identity: BBa J24708 sequence
display id: BBa_J24708_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24717_sequence/1
type: Sequence
persistent identity: BBa J24717 sequence
display id: BBa_J24717_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24727_sequence/1
type: Sequence
persistent identity: BBa J24727 sequence
display id: BBa_J24727_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24684_sequence/1
type: Sequence
persistent identity: BBa J24684 sequence
display id: BBa_J24684_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24686_sequence/1
type: Sequence
persistent identity: BBa J24686 sequence
display id: BBa_J24686_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24692_sequence/1
type: Sequence
persistent identity: BBa J24692 sequence
display id: BBa_J24692_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24695_sequence/1
type: Sequence
persistent identity: BBa J24695 sequence
display id: BBa_J24695_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24703_sequence/1
type: Sequence
persistent identity: BBa J24703 sequence
display id: BBa_J24703_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24705_sequence/1
type: Sequence
persistent identity: BBa J24705 sequence
display id: BBa_J24705_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24712_sequence/1
type: Sequence
persistent identity: BBa J24712 sequence
display id: BBa_J24712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24702_sequence/1
type: Sequence
persistent identity: BBa J24702 sequence
display id: BBa_J24702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24704_sequence/1
type: Sequence
persistent identity: BBa J24704 sequence
display id: BBa_J24704_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06505_sequence/1
type: Sequence
persistent identity: BBa J06505 sequence
display id: BBa_J06505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z