LOCUS BBa_J24786 9934 bp DNA linear 23-May-2024 DEFINITION 103. 72(BBa_J24753) == 58(BBa_J24738) ACCESSION BBa_J24786 VERSION BBa_J24786.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1103 /label=araC misc_feature 1104..1136 /label=LVA misc_feature 1143..1167 /label=Help:Barcodes CDS 228..1167 /label=BBa_C0080 misc_feature 1..1167 /label=BBa_J24692 misc_feature 1182..1185 /label=conserved RBS 1176..1189 /label=BBa_B0031 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1176..1880 /label=BBa_J24686 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1176..2766 /label=BBa_J24705 misc_feature 1..2766 /label=BBa_J24717 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 RBS 2837..2849 /label=RBS-3\Weak RBS 2843..2846 /label=RBS misc_feature 2837..2849 /label=BBa_B0032 RBS 2837..2849 /label=BBa_B0032 CDS 2856..3731 /label=araC misc_feature 3732..3764 /label=LVA misc_feature 3771..3795 /label=Help:Barcodes CDS 2856..3795 /label=BBa_C0080 misc_feature 2775..3795 /label=BBa_J24695 misc_feature 3808..3811 /label=conserved RBS 3804..3815 /label=BBa_B0034 misc_feature 4911..4949 /label=LVA CDS 3822..4949 /label=lacI-LVA misc_feature 3822..4949 /label=BBa_C0012 misc_feature 4950..4974 /label=Help:Barcodes CDS 3822..4949 /label=BBa_C0012 misc_feature 3804..4974 /label=BBa_J24685 misc_feature 4987..4990 /label=conserved RBS 4983..4994 /label=BBa_B0034 CDS 5001..5762 /label=YFP (LVA) misc_feature 5757..5762 /label=2 misc_feature 5718..5756 /label=SsrA misc_feature 5242..5242 /label=A (Q->L) misc_feature 5001..5762 /label=BBa_E0032 misc_feature 5001..5762 /label=BBa_E0032 stem_loop 5782..5825 /label=stem_loop misc_feature 5771..5850 /label=BBa_B0010 terminator 5771..5850 /label=BBa_B0010 stem_loop 5866..5885 /label=T7 TE misc_feature 5892..5892 /label=stop polyA_site 5886..5899 /label=polya misc_feature 5859..5899 /label=BBa_B0012 terminator 5859..5899 /label=BBa_B0012 misc_feature 4983..5899 /label=BBa_J24704 misc_feature 3804..5899 /label=BBa_J24713 misc_feature 2775..5899 /label=BBa_J24728 misc_feature 1..5899 /label=BBa_J24753 promoter 6987..7117 /label=Pbad promoter 6950..7021 /label=PC misc_feature 5908..6986 /label=AraC promoter 5908..7117 /label=BBa_I0500 misc_feature 7130..7133 /label=conserved RBS 7126..7137 /label=BBa_B0034 misc_feature 8233..8271 /label=LVA CDS 7144..8271 /label=lacI-LVA misc_feature 7144..8271 /label=BBa_C0012 misc_feature 8272..8296 /label=Help:Barcodes CDS 7144..8271 /label=BBa_C0012 misc_feature 7126..8296 /label=BBa_J24685 misc_feature 5908..8296 /label=BBa_J24699 misc_feature 8311..8314 /label=conserved RBS 8305..8318 /label=BBa_B0031 CDS 8328..8944 /label=tetR misc_feature 8945..8978 /label=SsrA misc_feature 8985..9009 /label=Help:Barcodes CDS 8325..9009 /label=BBa_C0040 misc_feature 8305..9009 /label=BBa_J24686 misc_feature 9022..9025 /label=conserved RBS 9018..9029 /label=BBa_B0034 CDS 9036..9797 /label=CFP (LVA) misc_feature 9754..9791 /label=SsrA misc_feature 9792..9797 /label=2 misc_feature 9104..9104 /label=A misc_feature 9036..9797 /label=BBa_E0022 misc_feature 9036..9797 /label=BBa_E0022 stem_loop 9817..9860 /label=stem_loop misc_feature 9806..9885 /label=BBa_B0010 terminator 9806..9885 /label=BBa_B0010 stem_loop 9901..9920 /label=T7 TE misc_feature 9927..9927 /label=stop polyA_site 9921..9934 /label=polya misc_feature 9894..9934 /label=BBa_B0012 terminator 9894..9934 /label=BBa_B0012 misc_feature 9018..9934 /label=BBa_J24703 misc_feature 8305..9934 /label=BBa_J24708 misc_feature 5908..9934 /label=BBa_J24738 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc 241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc 301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt 361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg 421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc 481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc 541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag 601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag 661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg 721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt 781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt 841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa 901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc 961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat 1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga 1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat 1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaacct actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaagt actagatggc tgaagcgcaa aatgatcccc 2881 tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca 2941 atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc 3001 tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg 3061 gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc 3121 gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta 3181 actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc 3241 atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg 3301 agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg 3361 agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc 3421 acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt 3481 cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg 3541 accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg 3601 tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca 3661 ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg 3721 tcaagttgtc agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 3781 gctagtgtag atcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag 3841 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3901 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3961 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 4021 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 4081 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 4141 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 4201 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 4261 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 4321 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 4381 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4441 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4501 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4561 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4621 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4681 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4741 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4801 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4861 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4921 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4981 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 5041 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5101 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5161 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc 5221 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg 5281 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5341 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5401 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 5461 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 5521 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5581 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 5641 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 5701 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 5761 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 5821 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 5881 gggcctttct gcgtttatat actagagtta tgacaacttg acggctacat cattcacttt 5941 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg 6001 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 6061 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac 6121 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg 6181 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 6241 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 6301 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 6361 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 6421 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 6481 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 6541 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc 6601 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 6661 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa 6721 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc 6781 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag 6841 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct 6901 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag 6961 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta 7021 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 7081 ctctactgtt tctccatacc cgtttttttg ggctagctac tagagaaaga ggagaaatac 7141 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 7201 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 7261 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 7321 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 7381 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 7441 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 7501 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 7561 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 7621 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 7681 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 7741 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 7801 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 7861 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 7921 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 7981 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 8041 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 8101 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 8161 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 8221 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 8281 tgctagtgta gatctctact agagtcacac aggaaaccta ctagatgtcc agattagata 8341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 8401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 8461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 8521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 8581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 8641 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 8701 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 8761 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 8821 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 8881 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 8941 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 9001 gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 9061 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 9121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 9181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg 9241 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 9301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 9361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 9421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc 9481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc 9541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 9601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 9661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 9721 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 9781 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 9841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 9901 ggctcacctt cgggtgggcc tttctgcgtt tata //