LOCUS BBa_J24789 9936 bp DNA linear 29-Nov-2024 DEFINITION 106. 79(BBa_J24759) == 52(BBa_J24732) ACCESSION BBa_J24789 VERSION BBa_J24789.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1181..1184 /label=conserved RBS 1175..1188 /label=BBa_B0031 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1175..1879 /label=BBa_J24686 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1175..2765 /label=BBa_J24705 misc_feature 1..2765 /label=BBa_J24720 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 misc_feature 2840..2843 /label=conserved RBS 2836..2847 /label=BBa_B0034 CDS 2854..3729 /label=araC misc_feature 3730..3762 /label=LVA misc_feature 3769..3793 /label=Help:Barcodes CDS 2854..3793 /label=BBa_C0080 misc_feature 2836..3793 /label=BBa_J24682 misc_feature 2774..3793 /label=BBa_J24696 misc_feature 3808..3811 /label=conserved RBS 3802..3815 /label=BBa_B0031 misc_feature 4911..4949 /label=LVA CDS 3822..4949 /label=lacI-LVA misc_feature 3822..4949 /label=BBa_C0012 misc_feature 4950..4974 /label=Help:Barcodes CDS 3822..4949 /label=BBa_C0012 misc_feature 3802..4974 /label=BBa_J24683 misc_feature 4987..4990 /label=conserved RBS 4983..4994 /label=BBa_B0034 CDS 5001..5762 /label=YFP (LVA) misc_feature 5757..5762 /label=2 misc_feature 5718..5756 /label=SsrA misc_feature 5242..5242 /label=A (Q->L) misc_feature 5001..5762 /label=BBa_E0032 misc_feature 5001..5762 /label=BBa_E0032 stem_loop 5782..5825 /label=stem_loop misc_feature 5771..5850 /label=BBa_B0010 terminator 5771..5850 /label=BBa_B0010 stem_loop 5866..5885 /label=T7 TE misc_feature 5892..5892 /label=stop polyA_site 5886..5899 /label=polya misc_feature 5859..5899 /label=BBa_B0012 terminator 5859..5899 /label=BBa_B0012 misc_feature 4983..5899 /label=BBa_J24704 misc_feature 3802..5899 /label=BBa_J24711 misc_feature 2774..5899 /label=BBa_J24729 misc_feature 1..5899 /label=BBa_J24759 promoter 6987..7117 /label=Pbad promoter 6950..7021 /label=PC misc_feature 5908..6986 /label=AraC promoter 5908..7117 /label=BBa_I0500 misc_feature 7132..7135 /label=conserved RBS 7126..7139 /label=BBa_B0031 misc_feature 8235..8273 /label=LVA CDS 7146..8273 /label=lacI-LVA misc_feature 7146..8273 /label=BBa_C0012 misc_feature 8274..8298 /label=Help:Barcodes CDS 7146..8273 /label=BBa_C0012 misc_feature 7126..8298 /label=BBa_J24683 misc_feature 5908..8298 /label=BBa_J24697 misc_feature 8313..8316 /label=conserved RBS 8307..8320 /label=BBa_B0031 CDS 8330..8946 /label=tetR misc_feature 8947..8980 /label=SsrA misc_feature 8987..9011 /label=Help:Barcodes CDS 8327..9011 /label=BBa_C0040 misc_feature 8307..9011 /label=BBa_J24686 misc_feature 9024..9027 /label=conserved RBS 9020..9031 /label=BBa_B0034 CDS 9038..9799 /label=CFP (LVA) misc_feature 9756..9793 /label=SsrA misc_feature 9794..9799 /label=2 misc_feature 9106..9106 /label=A misc_feature 9038..9799 /label=BBa_E0022 misc_feature 9038..9799 /label=BBa_E0022 stem_loop 9819..9862 /label=stem_loop misc_feature 9808..9887 /label=BBa_B0010 terminator 9808..9887 /label=BBa_B0010 stem_loop 9903..9922 /label=T7 TE misc_feature 9929..9929 /label=stop polyA_site 9923..9936 /label=polya misc_feature 9896..9936 /label=BBa_B0012 terminator 9896..9936 /label=BBa_B0012 misc_feature 9020..9936 /label=BBa_J24703 misc_feature 8307..9936 /label=BBa_J24708 misc_feature 5908..9936 /label=BBa_J24732 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaaccta ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagaaaga ggagaaatac tagatggctg aagcgcaaaa tgatcccctg 2881 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat 2941 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc 3001 accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt 3061 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc 3121 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac 3181 tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat 3241 ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag 3301 ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag 3361 tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac 3421 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg 3481 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac 3541 caacgcatca gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc 3601 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc 3661 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc 3721 aagttgtcag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 3781 tagtgtagat cactactaga gtcacacagg aaacctacta gatggtgaat gtgaaaccag 3841 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3901 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3961 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 4021 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 4081 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 4141 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 4201 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 4261 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 4321 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 4381 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4441 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4501 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4561 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4621 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4681 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4741 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4801 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4861 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4921 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4981 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 5041 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5101 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5161 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcaa tgcttcgccc 5221 gctaccccga ccacatgaag ctgcacgact tcttcaagtc cgccatgccc gaaggctacg 5281 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5341 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5401 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 5461 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 5521 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5581 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 5641 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 5701 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 5761 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 5821 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 5881 gggcctttct gcgtttatat actagagtta tgacaacttg acggctacat cattcacttt 5941 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg 6001 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 6061 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac 6121 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg 6181 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 6241 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 6301 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 6361 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 6421 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 6481 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 6541 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc 6601 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 6661 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa 6721 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc 6781 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag 6841 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct 6901 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag 6961 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta 7021 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 7081 ctctactgtt tctccatacc cgtttttttg ggctagctac tagagtcaca caggaaacct 7141 actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 7201 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 7261 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 7321 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 7381 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 7441 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 7501 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 7561 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 7621 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 7681 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 7741 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 7801 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 7861 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 7921 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 7981 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 8041 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 8101 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 8161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 8221 tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 8281 agtgctagtg tagatctcta ctagagtcac acaggaaacc tactagatgt ccagattaga 8341 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt 8401 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt 8461 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac 8521 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag 8581 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc 8641 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt 8701 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt 8761 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag 8821 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc 8881 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag 8941 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 9001 gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct 9061 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt 9121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 9181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg 9241 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 9301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 9361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 9421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag 9481 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 9541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 9601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 9661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 9721 cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg acgaaaacta 9781 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 9841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 9901 ctggctcacc ttcgggtggg cctttctgcg tttata //