LOCUS BBa_J24792 9934 bp DNA linear 22-Nov-2024 DEFINITION 109. 80(BBa_J24760) == 55(BBa_J24735) ACCESSION BBa_J24792 VERSION BBa_J24792.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1181..1184 /label=conserved RBS 1175..1188 /label=BBa_B0031 CDS 1198..1814 /label=tetR misc_feature 1815..1848 /label=SsrA misc_feature 1855..1879 /label=Help:Barcodes CDS 1195..1879 /label=BBa_C0040 misc_feature 1175..1879 /label=BBa_J24686 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2613 /label=mCherry misc_feature 2257..2257 /label=C->T (removing PstI site) misc_feature 2614..2622 /label=LVA misc_feature 1906..2628 /label=BBa_J06505 stem_loop 2648..2691 /label=stem_loop misc_feature 2637..2716 /label=BBa_B0010 terminator 2637..2716 /label=BBa_B0010 stem_loop 2732..2751 /label=T7 TE misc_feature 2758..2758 /label=stop polyA_site 2752..2765 /label=polya misc_feature 2725..2765 /label=BBa_B0012 terminator 2725..2765 /label=BBa_B0012 misc_feature 1888..2765 /label=BBa_J24702 misc_feature 1175..2765 /label=BBa_J24705 misc_feature 1..2765 /label=BBa_J24720 misc_feature 2774..2792 /label=TetR 1 misc_feature 2774..2827 /label=BBa_R0040 promoter 2793..2798 /label=-35 misc_feature 2799..2817 /label=TetR 2 promoter 2816..2821 /label=-10 promoter 2774..2827 /label=BBa_R0040 misc_feature 2840..2843 /label=conserved RBS 2836..2847 /label=BBa_B0034 CDS 2854..3729 /label=araC misc_feature 3730..3762 /label=LVA misc_feature 3769..3793 /label=Help:Barcodes CDS 2854..3793 /label=BBa_C0080 misc_feature 2836..3793 /label=BBa_J24682 misc_feature 2774..3793 /label=BBa_J24696 RBS 3802..3814 /label=RBS-3\Weak RBS 3808..3811 /label=RBS misc_feature 3802..3814 /label=BBa_B0032 RBS 3802..3814 /label=BBa_B0032 misc_feature 4910..4948 /label=LVA CDS 3821..4948 /label=lacI-LVA misc_feature 3821..4948 /label=BBa_C0012 misc_feature 4949..4973 /label=Help:Barcodes CDS 3821..4948 /label=BBa_C0012 misc_feature 3802..4973 /label=BBa_J24684 misc_feature 4986..4989 /label=conserved RBS 4982..4993 /label=BBa_B0034 CDS 5000..5761 /label=YFP (LVA) misc_feature 5756..5761 /label=2 misc_feature 5717..5755 /label=SsrA misc_feature 5241..5241 /label=A (Q->L) misc_feature 5000..5761 /label=BBa_E0032 misc_feature 5000..5761 /label=BBa_E0032 stem_loop 5781..5824 /label=stem_loop misc_feature 5770..5849 /label=BBa_B0010 terminator 5770..5849 /label=BBa_B0010 stem_loop 5865..5884 /label=T7 TE misc_feature 5891..5891 /label=stop polyA_site 5885..5898 /label=polya misc_feature 5858..5898 /label=BBa_B0012 terminator 5858..5898 /label=BBa_B0012 misc_feature 4982..5898 /label=BBa_J24704 misc_feature 3802..5898 /label=BBa_J24712 misc_feature 2774..5898 /label=BBa_J24730 misc_feature 1..5898 /label=BBa_J24760 promoter 6986..7116 /label=Pbad promoter 6949..7020 /label=PC misc_feature 5907..6985 /label=AraC promoter 5907..7116 /label=BBa_I0500 RBS 7125..7137 /label=RBS-3\Weak RBS 7131..7134 /label=RBS misc_feature 7125..7137 /label=BBa_B0032 RBS 7125..7137 /label=BBa_B0032 misc_feature 8233..8271 /label=LVA CDS 7144..8271 /label=lacI-LVA misc_feature 7144..8271 /label=BBa_C0012 misc_feature 8272..8296 /label=Help:Barcodes CDS 7144..8271 /label=BBa_C0012 misc_feature 7125..8296 /label=BBa_J24684 misc_feature 5907..8296 /label=BBa_J24698 misc_feature 8311..8314 /label=conserved RBS 8305..8318 /label=BBa_B0031 CDS 8328..8944 /label=tetR misc_feature 8945..8978 /label=SsrA misc_feature 8985..9009 /label=Help:Barcodes CDS 8325..9009 /label=BBa_C0040 misc_feature 8305..9009 /label=BBa_J24686 misc_feature 9022..9025 /label=conserved RBS 9018..9029 /label=BBa_B0034 CDS 9036..9797 /label=CFP (LVA) misc_feature 9754..9791 /label=SsrA misc_feature 9792..9797 /label=2 misc_feature 9104..9104 /label=A misc_feature 9036..9797 /label=BBa_E0022 misc_feature 9036..9797 /label=BBa_E0022 stem_loop 9817..9860 /label=stem_loop misc_feature 9806..9885 /label=BBa_B0010 terminator 9806..9885 /label=BBa_B0010 stem_loop 9901..9920 /label=T7 TE misc_feature 9927..9927 /label=stop polyA_site 9921..9934 /label=polya misc_feature 9894..9934 /label=BBa_B0012 terminator 9894..9934 /label=BBa_B0012 misc_feature 9018..9934 /label=BBa_J24703 misc_feature 8305..9934 /label=BBa_J24708 misc_feature 5907..9934 /label=BBa_J24735 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaaccta ctagatgtcc 1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac 2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag 2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2761 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac 2821 tgagcactac tagagaaaga ggagaaatac tagatggctg aagcgcaaaa tgatcccctg 2881 ctgccgggat actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat 2941 ggttatctcg atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc 3001 accattcgcg gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt 3061 gatattttgc tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc 3121 gaatggtatc accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac 3181 tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat 3241 ttcagcgacc tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag 3301 ctgctggcga taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag 3361 tcgctccatc caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac 3421 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg 3481 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac 3541 caacgcatca gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc 3601 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc 3661 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc 3721 aagttgtcag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 3781 tagtgtagat cactactaga gtcacacagg aaagtactag atggtgaatg tgaaaccagt 3841 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 3901 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 3961 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 4021 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 4081 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 4141 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 4201 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 4261 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 4321 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 4381 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 4441 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 4501 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 4561 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 4621 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 4681 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 4741 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 4801 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 4861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga 4921 cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga 4981 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 5041 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 5101 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 5161 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 5221 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 5281 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 5341 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 5401 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 5461 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 5521 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 5581 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 5641 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 5701 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 5761 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 5821 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 5881 ggcctttctg cgtttatata ctagagttat gacaacttga cggctacatc attcactttt 5941 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc 6001 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg 6061 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg 6121 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc 6181 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa 6241 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc 6301 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct 6361 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc 6421 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag 6481 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg 6541 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct 6601 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc 6661 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa 6721 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca 6781 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga 6841 cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt 6901 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt 6961 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat 7021 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc 7081 tctactgttt ctccataccc gtttttttgg gctagctact agagtcacac aggaaagtac 7141 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 7201 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 7261 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 7321 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 7381 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 7441 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 7501 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 7561 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 7621 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 7681 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 7741 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 7801 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 7861 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 7921 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 7981 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 8041 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 8101 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 8161 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 8221 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 8281 tgctagtgta gatctctact agagtcacac aggaaaccta ctagatgtcc agattagata 8341 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa 8401 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa 8461 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc 8521 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt 8581 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta 8641 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt 8701 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat 8761 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta 8821 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct 8881 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg 8941 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt 9001 gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 9061 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 9121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 9181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg 9241 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 9301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 9361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 9421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc 9481 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc 9541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 9601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 9661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 9721 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 9781 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 9841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 9901 ggctcacctt cgggtgggcc tttctgcgtt tata //