LOCUS BBa_J24793 9932 bp DNA linear 26-Nov-2024 DEFINITION 110. 83(BBa_J24763) == 56(BBa_J24736) ACCESSION BBa_J24793 VERSION BBa_J24793.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 RBS 1175..1187 /label=RBS-3\Weak RBS 1181..1184 /label=RBS misc_feature 1175..1187 /label=BBa_B0032 RBS 1175..1187 /label=BBa_B0032 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1175..1878 /label=BBa_J24687 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1175..2764 /label=BBa_J24706 misc_feature 1..2764 /label=BBa_J24721 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 misc_feature 2839..2842 /label=conserved RBS 2835..2846 /label=BBa_B0034 CDS 2853..3728 /label=araC misc_feature 3729..3761 /label=LVA misc_feature 3768..3792 /label=Help:Barcodes CDS 2853..3792 /label=BBa_C0080 misc_feature 2835..3792 /label=BBa_J24682 misc_feature 2773..3792 /label=BBa_J24696 RBS 3801..3813 /label=RBS-3\Weak RBS 3807..3810 /label=RBS misc_feature 3801..3813 /label=BBa_B0032 RBS 3801..3813 /label=BBa_B0032 misc_feature 4909..4947 /label=LVA CDS 3820..4947 /label=lacI-LVA misc_feature 3820..4947 /label=BBa_C0012 misc_feature 4948..4972 /label=Help:Barcodes CDS 3820..4947 /label=BBa_C0012 misc_feature 3801..4972 /label=BBa_J24684 misc_feature 4985..4988 /label=conserved RBS 4981..4992 /label=BBa_B0034 CDS 4999..5760 /label=YFP (LVA) misc_feature 5755..5760 /label=2 misc_feature 5716..5754 /label=SsrA misc_feature 5240..5240 /label=A (Q->L) misc_feature 4999..5760 /label=BBa_E0032 misc_feature 4999..5760 /label=BBa_E0032 stem_loop 5780..5823 /label=stem_loop misc_feature 5769..5848 /label=BBa_B0010 terminator 5769..5848 /label=BBa_B0010 stem_loop 5864..5883 /label=T7 TE misc_feature 5890..5890 /label=stop polyA_site 5884..5897 /label=polya misc_feature 5857..5897 /label=BBa_B0012 terminator 5857..5897 /label=BBa_B0012 misc_feature 4981..5897 /label=BBa_J24704 misc_feature 3801..5897 /label=BBa_J24712 misc_feature 2773..5897 /label=BBa_J24730 misc_feature 1..5897 /label=BBa_J24763 promoter 6985..7115 /label=Pbad promoter 6948..7019 /label=PC misc_feature 5906..6984 /label=AraC promoter 5906..7115 /label=BBa_I0500 RBS 7124..7136 /label=RBS-3\Weak RBS 7130..7133 /label=RBS misc_feature 7124..7136 /label=BBa_B0032 RBS 7124..7136 /label=BBa_B0032 misc_feature 8232..8270 /label=LVA CDS 7143..8270 /label=lacI-LVA misc_feature 7143..8270 /label=BBa_C0012 misc_feature 8271..8295 /label=Help:Barcodes CDS 7143..8270 /label=BBa_C0012 misc_feature 7124..8295 /label=BBa_J24684 misc_feature 5906..8295 /label=BBa_J24698 RBS 8304..8316 /label=RBS-3\Weak RBS 8310..8313 /label=RBS misc_feature 8304..8316 /label=BBa_B0032 RBS 8304..8316 /label=BBa_B0032 CDS 8326..8942 /label=tetR misc_feature 8943..8976 /label=SsrA misc_feature 8983..9007 /label=Help:Barcodes CDS 8323..9007 /label=BBa_C0040 misc_feature 8304..9007 /label=BBa_J24687 misc_feature 9020..9023 /label=conserved RBS 9016..9027 /label=BBa_B0034 CDS 9034..9795 /label=CFP (LVA) misc_feature 9752..9789 /label=SsrA misc_feature 9790..9795 /label=2 misc_feature 9102..9102 /label=A misc_feature 9034..9795 /label=BBa_E0022 misc_feature 9034..9795 /label=BBa_E0022 stem_loop 9815..9858 /label=stem_loop misc_feature 9804..9883 /label=BBa_B0010 terminator 9804..9883 /label=BBa_B0010 stem_loop 9899..9918 /label=T7 TE misc_feature 9925..9925 /label=stop polyA_site 9919..9932 /label=polya misc_feature 9892..9932 /label=BBa_B0012 terminator 9892..9932 /label=BBa_B0012 misc_feature 9016..9932 /label=BBa_J24703 misc_feature 8304..9932 /label=BBa_J24709 misc_feature 5906..9932 /label=BBa_J24736 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaagtac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc 2881 tgccgggata ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg 2941 gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca 3001 ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg 3061 atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg 3121 aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact 3181 ggccgtcaat atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt 3241 tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc 3301 tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt 3361 cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc 3421 tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc 3481 gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc 3541 aacgcatcag ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg 3601 gtcgcaatgt tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg 3661 gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca 3721 agttgtcagc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 3781 agtgtagatc actactagag tcacacagga aagtactaga tggtgaatgt gaaaccagta 3841 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 3901 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3961 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 4021 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 4081 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 4141 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 4201 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 4261 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 4321 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 4381 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 4441 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 4501 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 4561 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 4621 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 4681 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 4741 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 4801 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 4921 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4981 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 5041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 5101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 5161 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc 5221 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 5281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 5341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 5401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 5461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 5521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 5581 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 5641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 5701 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa 5761 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 5821 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 5881 gcctttctgc gtttatatac tagagttatg acaacttgac ggctacatca ttcacttttt 5941 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 6001 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 6061 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 6121 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 6181 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 6241 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 6301 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 6361 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 6421 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 6481 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 6541 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 6601 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 6661 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 6721 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 6781 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 6841 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 6901 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 6961 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 7021 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 7081 ctactgtttc tccatacccg tttttttggg ctagctacta gagtcacaca ggaaagtact 7141 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 7201 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 7261 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 7321 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 7381 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 7441 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 7501 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 7561 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 7621 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 7681 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 7741 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 7801 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 7861 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 7921 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 7981 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 8041 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 8101 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 8161 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 8221 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 8281 gctagtgtag atctctacta gagtcacaca ggaaagtact agatgtccag attagataaa 8341 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca 8401 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa 8461 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac 8521 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt 8581 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca 8641 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca 8701 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg 8761 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg 8821 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc 8881 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg 8941 tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt 9001 agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc 9061 accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca caagttcagc 9121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 9181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctggggcgtg 9241 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 9301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 9361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 9421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta catcagccac 9481 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc 9541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 9601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 9661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 9721 atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct 9781 ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 9841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 9901 ctcaccttcg ggtgggcctt tctgcgttta ta //