LOCUS BBa_J24794 9930 bp DNA linear 18-May-2024 DEFINITION 111. 86(BBa_J24766) == 57(BBa_J24737) ACCESSION BBa_J24794 VERSION BBa_J24794.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 misc_feature 1179..1182 /label=conserved RBS 1175..1186 /label=BBa_B0034 CDS 1196..1812 /label=tetR misc_feature 1813..1846 /label=SsrA misc_feature 1853..1877 /label=Help:Barcodes CDS 1193..1877 /label=BBa_C0040 misc_feature 1175..1877 /label=BBa_J24688 misc_feature 1890..1893 /label=conserved RBS 1886..1897 /label=BBa_B0034 CDS 1904..2611 /label=mCherry misc_feature 2255..2255 /label=C->T (removing PstI site) misc_feature 2612..2620 /label=LVA misc_feature 1904..2626 /label=BBa_J06505 stem_loop 2646..2689 /label=stem_loop misc_feature 2635..2714 /label=BBa_B0010 terminator 2635..2714 /label=BBa_B0010 stem_loop 2730..2749 /label=T7 TE misc_feature 2756..2756 /label=stop polyA_site 2750..2763 /label=polya misc_feature 2723..2763 /label=BBa_B0012 terminator 2723..2763 /label=BBa_B0012 misc_feature 1886..2763 /label=BBa_J24702 misc_feature 1175..2763 /label=BBa_J24707 misc_feature 1..2763 /label=BBa_J24722 misc_feature 2772..2790 /label=TetR 1 misc_feature 2772..2825 /label=BBa_R0040 promoter 2791..2796 /label=-35 misc_feature 2797..2815 /label=TetR 2 promoter 2814..2819 /label=-10 promoter 2772..2825 /label=BBa_R0040 misc_feature 2838..2841 /label=conserved RBS 2834..2845 /label=BBa_B0034 CDS 2852..3727 /label=araC misc_feature 3728..3760 /label=LVA misc_feature 3767..3791 /label=Help:Barcodes CDS 2852..3791 /label=BBa_C0080 misc_feature 2834..3791 /label=BBa_J24682 misc_feature 2772..3791 /label=BBa_J24696 RBS 3800..3812 /label=RBS-3\Weak RBS 3806..3809 /label=RBS misc_feature 3800..3812 /label=BBa_B0032 RBS 3800..3812 /label=BBa_B0032 misc_feature 4908..4946 /label=LVA CDS 3819..4946 /label=lacI-LVA misc_feature 3819..4946 /label=BBa_C0012 misc_feature 4947..4971 /label=Help:Barcodes CDS 3819..4946 /label=BBa_C0012 misc_feature 3800..4971 /label=BBa_J24684 misc_feature 4984..4987 /label=conserved RBS 4980..4991 /label=BBa_B0034 CDS 4998..5759 /label=YFP (LVA) misc_feature 5754..5759 /label=2 misc_feature 5715..5753 /label=SsrA misc_feature 5239..5239 /label=A (Q->L) misc_feature 4998..5759 /label=BBa_E0032 misc_feature 4998..5759 /label=BBa_E0032 stem_loop 5779..5822 /label=stem_loop misc_feature 5768..5847 /label=BBa_B0010 terminator 5768..5847 /label=BBa_B0010 stem_loop 5863..5882 /label=T7 TE misc_feature 5889..5889 /label=stop polyA_site 5883..5896 /label=polya misc_feature 5856..5896 /label=BBa_B0012 terminator 5856..5896 /label=BBa_B0012 misc_feature 4980..5896 /label=BBa_J24704 misc_feature 3800..5896 /label=BBa_J24712 misc_feature 2772..5896 /label=BBa_J24730 misc_feature 1..5896 /label=BBa_J24766 promoter 6984..7114 /label=Pbad promoter 6947..7018 /label=PC misc_feature 5905..6983 /label=AraC promoter 5905..7114 /label=BBa_I0500 RBS 7123..7135 /label=RBS-3\Weak RBS 7129..7132 /label=RBS misc_feature 7123..7135 /label=BBa_B0032 RBS 7123..7135 /label=BBa_B0032 misc_feature 8231..8269 /label=LVA CDS 7142..8269 /label=lacI-LVA misc_feature 7142..8269 /label=BBa_C0012 misc_feature 8270..8294 /label=Help:Barcodes CDS 7142..8269 /label=BBa_C0012 misc_feature 7123..8294 /label=BBa_J24684 misc_feature 5905..8294 /label=BBa_J24698 misc_feature 8307..8310 /label=conserved RBS 8303..8314 /label=BBa_B0034 CDS 8324..8940 /label=tetR misc_feature 8941..8974 /label=SsrA misc_feature 8981..9005 /label=Help:Barcodes CDS 8321..9005 /label=BBa_C0040 misc_feature 8303..9005 /label=BBa_J24688 misc_feature 9018..9021 /label=conserved RBS 9014..9025 /label=BBa_B0034 CDS 9032..9793 /label=CFP (LVA) misc_feature 9750..9787 /label=SsrA misc_feature 9788..9793 /label=2 misc_feature 9100..9100 /label=A misc_feature 9032..9793 /label=BBa_E0022 misc_feature 9032..9793 /label=BBa_E0022 stem_loop 9813..9856 /label=stem_loop misc_feature 9802..9881 /label=BBa_B0010 terminator 9802..9881 /label=BBa_B0010 stem_loop 9897..9916 /label=T7 TE misc_feature 9923..9923 /label=stop polyA_site 9917..9930 /label=polya misc_feature 9890..9930 /label=BBa_B0012 terminator 9890..9930 /label=BBa_B0012 misc_feature 9014..9930 /label=BBa_J24703 misc_feature 8303..9930 /label=BBa_J24710 misc_feature 5905..9930 /label=BBa_J24737 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatgtccag 1201 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga 1261 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg 1321 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca 1381 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc 1441 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac 1501 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca 1561 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg 1621 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac 1681 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga 1741 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg 1801 tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata 1861 gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga gcaagggcga 1921 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 1981 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2041 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2101 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2161 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2221 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa 2281 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 2341 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 2401 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 2461 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 2521 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 2581 ctccaccggc ggcatggacg agctgtacaa gttagtagct taataatact agagccaggc 2641 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2701 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2761 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 2821 agcactacta gagaaagagg agaaatacta gatggctgaa gcgcaaaatg atcccctgct 2881 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaatgg 2941 ttatctcgat ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac 3001 cattcgcggt cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga 3061 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga 3121 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg 3181 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt 3241 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct 3301 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc 3361 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct 3421 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg 3481 tctgtcacat cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca 3541 acgcatcagc caggcgaagc tgcttttgag cactacccgg atgcctatcg ccaccgtcgg 3601 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg 3661 ggccagcccg agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa 3721 gttgtcagct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 3781 gtgtagatca ctactagagt cacacaggaa agtactagat ggtgaatgtg aaaccagtaa 3841 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 3901 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 3961 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 4021 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 4081 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 4141 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 4201 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 4261 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 4321 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 4381 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 4441 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 4501 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 4561 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 4621 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 4681 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 4741 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 4801 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg 4921 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 4981 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 5041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 5101 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 5161 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct 5221 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 5281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 5341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 5401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 5461 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 5521 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 5581 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 5641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 5701 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat 5761 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5821 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5881 cctttctgcg tttatatact agagttatga caacttgacg gctacatcat tcactttttc 5941 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga 6001 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt 6061 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct 6121 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg 6181 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc 6241 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg 6301 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg 6361 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg 6421 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc 6481 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta 6541 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg 6601 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat 6661 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc 6721 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc 6781 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca 6841 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat 6901 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt 6961 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc 7021 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc 7081 tactgtttct ccatacccgt ttttttgggc tagctactag agtcacacag gaaagtacta 7141 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 7201 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 7261 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 7321 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 7381 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 7441 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 7501 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 7561 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 7621 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 7681 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 7741 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 7801 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 7861 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 7921 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 7981 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 8041 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 8101 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 8161 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 8221 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 8281 ctagtgtaga tctctactag agaaagagga gaaatactag atgtccagat tagataaaag 8341 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 8401 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 8461 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 8521 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 8581 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 8641 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 8701 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 8761 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 8821 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 8881 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 8941 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 9001 atcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 9061 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt 9121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 9181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca 9241 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 9301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 9361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 9421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa 9481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 9541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 9601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 9661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 9721 cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt 9781 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 9841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 9901 caccttcggg tgggcctttc tgcgtttata //