LOCUS BBa_J24796 9930 bp DNA linear 23-May-2024 DEFINITION 113. 84(BBa_J24764) == 59(BBa_J24739) ACCESSION BBa_J24796 VERSION BBa_J24796.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1102 /label=araC misc_feature 1103..1135 /label=LVA misc_feature 1142..1166 /label=Help:Barcodes CDS 227..1166 /label=BBa_C0080 misc_feature 1..1166 /label=BBa_J24693 RBS 1175..1187 /label=RBS-3\Weak RBS 1181..1184 /label=RBS misc_feature 1175..1187 /label=BBa_B0032 RBS 1175..1187 /label=BBa_B0032 CDS 1197..1813 /label=tetR misc_feature 1814..1847 /label=SsrA misc_feature 1854..1878 /label=Help:Barcodes CDS 1194..1878 /label=BBa_C0040 misc_feature 1175..1878 /label=BBa_J24687 misc_feature 1891..1894 /label=conserved RBS 1887..1898 /label=BBa_B0034 CDS 1905..2612 /label=mCherry misc_feature 2256..2256 /label=C->T (removing PstI site) misc_feature 2613..2621 /label=LVA misc_feature 1905..2627 /label=BBa_J06505 stem_loop 2647..2690 /label=stem_loop misc_feature 2636..2715 /label=BBa_B0010 terminator 2636..2715 /label=BBa_B0010 stem_loop 2731..2750 /label=T7 TE misc_feature 2757..2757 /label=stop polyA_site 2751..2764 /label=polya misc_feature 2724..2764 /label=BBa_B0012 terminator 2724..2764 /label=BBa_B0012 misc_feature 1887..2764 /label=BBa_J24702 misc_feature 1175..2764 /label=BBa_J24706 misc_feature 1..2764 /label=BBa_J24721 misc_feature 2773..2791 /label=TetR 1 misc_feature 2773..2826 /label=BBa_R0040 promoter 2792..2797 /label=-35 misc_feature 2798..2816 /label=TetR 2 promoter 2815..2820 /label=-10 promoter 2773..2826 /label=BBa_R0040 misc_feature 2839..2842 /label=conserved RBS 2835..2846 /label=BBa_B0034 CDS 2853..3728 /label=araC misc_feature 3729..3761 /label=LVA misc_feature 3768..3792 /label=Help:Barcodes CDS 2853..3792 /label=BBa_C0080 misc_feature 2835..3792 /label=BBa_J24682 misc_feature 2773..3792 /label=BBa_J24696 misc_feature 3805..3808 /label=conserved RBS 3801..3812 /label=BBa_B0034 misc_feature 4908..4946 /label=LVA CDS 3819..4946 /label=lacI-LVA misc_feature 3819..4946 /label=BBa_C0012 misc_feature 4947..4971 /label=Help:Barcodes CDS 3819..4946 /label=BBa_C0012 misc_feature 3801..4971 /label=BBa_J24685 misc_feature 4984..4987 /label=conserved RBS 4980..4991 /label=BBa_B0034 CDS 4998..5759 /label=YFP (LVA) misc_feature 5754..5759 /label=2 misc_feature 5715..5753 /label=SsrA misc_feature 5239..5239 /label=A (Q->L) misc_feature 4998..5759 /label=BBa_E0032 misc_feature 4998..5759 /label=BBa_E0032 stem_loop 5779..5822 /label=stem_loop misc_feature 5768..5847 /label=BBa_B0010 terminator 5768..5847 /label=BBa_B0010 stem_loop 5863..5882 /label=T7 TE misc_feature 5889..5889 /label=stop polyA_site 5883..5896 /label=polya misc_feature 5856..5896 /label=BBa_B0012 terminator 5856..5896 /label=BBa_B0012 misc_feature 4980..5896 /label=BBa_J24704 misc_feature 3801..5896 /label=BBa_J24713 misc_feature 2773..5896 /label=BBa_J24731 misc_feature 1..5896 /label=BBa_J24764 promoter 6984..7114 /label=Pbad promoter 6947..7018 /label=PC misc_feature 5905..6983 /label=AraC promoter 5905..7114 /label=BBa_I0500 misc_feature 7127..7130 /label=conserved RBS 7123..7134 /label=BBa_B0034 misc_feature 8230..8268 /label=LVA CDS 7141..8268 /label=lacI-LVA misc_feature 7141..8268 /label=BBa_C0012 misc_feature 8269..8293 /label=Help:Barcodes CDS 7141..8268 /label=BBa_C0012 misc_feature 7123..8293 /label=BBa_J24685 misc_feature 5905..8293 /label=BBa_J24699 RBS 8302..8314 /label=RBS-3\Weak RBS 8308..8311 /label=RBS misc_feature 8302..8314 /label=BBa_B0032 RBS 8302..8314 /label=BBa_B0032 CDS 8324..8940 /label=tetR misc_feature 8941..8974 /label=SsrA misc_feature 8981..9005 /label=Help:Barcodes CDS 8321..9005 /label=BBa_C0040 misc_feature 8302..9005 /label=BBa_J24687 misc_feature 9018..9021 /label=conserved RBS 9014..9025 /label=BBa_B0034 CDS 9032..9793 /label=CFP (LVA) misc_feature 9750..9787 /label=SsrA misc_feature 9788..9793 /label=2 misc_feature 9100..9100 /label=A misc_feature 9032..9793 /label=BBa_E0022 misc_feature 9032..9793 /label=BBa_E0022 stem_loop 9813..9856 /label=stem_loop misc_feature 9802..9881 /label=BBa_B0010 terminator 9802..9881 /label=BBa_B0010 stem_loop 9897..9916 /label=T7 TE misc_feature 9923..9923 /label=stop polyA_site 9917..9930 /label=polya misc_feature 9890..9930 /label=BBa_B0012 terminator 9890..9930 /label=BBa_B0012 misc_feature 9014..9930 /label=BBa_J24703 misc_feature 8302..9930 /label=BBa_J24709 misc_feature 5905..9930 /label=BBa_J24739 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctgaagcgca 241 aaatgatccc ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc 301 gattgaggcc aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta 361 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt 421 ctgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca 481 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca 541 tgaatggctt aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc 601 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg 661 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga 721 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta 781 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg 841 cctgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag 901 ctggcgcgag gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc 961 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt 1021 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa 1081 tgatgtagcc gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata 1141 acactgatag tgctagtgta gatcactact agagtcacac aggaaagtac tagatgtcca 1201 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg 1261 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt 1321 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc 1381 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg 1441 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta 1501 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac 1561 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag 1621 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta 1681 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag 1741 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat 1801 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1861 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatggtg agcaagggcg 1921 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1981 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2041 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 2101 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2161 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2221 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca 2281 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2341 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2401 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2461 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2521 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2581 actccaccgg cggcatggac gagctgtaca agttagtagc ttaataatac tagagccagg 2641 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2701 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2761 tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact 2821 gagcactact agagaaagag gagaaatact agatggctga agcgcaaaat gatcccctgc 2881 tgccgggata ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg 2941 gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca 3001 ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg 3061 atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg 3121 aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact 3181 ggccgtcaat atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt 3241 tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc 3301 tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt 3361 cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc 3421 tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc 3481 gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc 3541 aacgcatcag ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg 3601 gtcgcaatgt tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg 3661 gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca 3721 agttgtcagc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 3781 agtgtagatc actactagag aaagaggaga aatactagat ggtgaatgtg aaaccagtaa 3841 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 3901 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 3961 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 4021 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 4081 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 4141 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 4201 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 4261 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 4321 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 4381 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 4441 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 4501 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 4561 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 4621 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 4681 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 4741 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 4801 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg 4921 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagaga 4981 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 5041 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 5101 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 5161 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct 5221 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 5281 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 5341 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 5401 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 5461 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 5521 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 5581 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 5641 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 5701 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat 5761 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5821 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5881 cctttctgcg tttatatact agagttatga caacttgacg gctacatcat tcactttttc 5941 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga 6001 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt 6061 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct 6121 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg 6181 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc 6241 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg 6301 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg 6361 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg 6421 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc 6481 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta 6541 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg 6601 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat 6661 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc 6721 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc 6781 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca 6841 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat 6901 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt 6961 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc 7021 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc 7081 tactgtttct ccatacccgt ttttttgggc tagctactag agaaagagga gaaatactag 7141 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 7201 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 7261 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 7321 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 7381 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 7441 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 7501 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 7561 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 7621 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 7681 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 7741 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 7801 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 7861 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 7921 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 7981 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 8041 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 8101 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 8161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 8221 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 8281 tagtgtagat ctctactaga gtcacacagg aaagtactag atgtccagat tagataaaag 8341 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac 8401 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa 8461 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt 8521 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag 8581 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga 8641 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact 8701 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga 8761 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc 8821 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt 8881 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc 8941 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 9001 atcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 9061 cggggtggtg cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt 9121 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 9181 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca 9241 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 9301 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 9361 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 9421 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa 9481 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 9541 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 9601 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 9661 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 9721 cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt 9781 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 9841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 9901 caccttcggg tgggcctttc tgcgtttata //